OnePot PURE Cell-Free System

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One of the goals of synthetic biology is the development of an artificial cell. Building an artificial cell from scratch will provide a deeper understanding of fundamental mechanisms and models in biology and promises to contribute towards building novel p ...

Generative language models (LMs) have become omnipresent across data science. For a wide variety of tasks, inputs can be phrased as natural language prompts for an LM, from whose output the solution can then be extracted. LM performance has consistently be ...

Time has always been a central factor in understanding the challenges of daily mobility. For a long time, and still today, methods of economic evaluation of transport projects have monetized time savings so that they can be included in the cost–benefit ana ...

Increasing the protein production capacity of the PURE cell-free transcription-translation (TX-TL) system will be key to implementing complex synthetic biological circuits, and to establishing a fully self-regenerating system as a basis for the development ...

Host attachment is often a critical step in the onset of pathogenesis. To attach to host cells, bacteria have evolved a range of adhesins that bind to specific receptors. Some of these adhesins have been thoroughly characterized using biochemical technique ...

Cell-free biology is increasingly utilized for engineering biological systems, incorporating novel functionality, and circumventing many of the complications associated with cells. The central dogma describes the information flow in biology consisting of t ...

Three different power-to-methane process chains with grid injection in two scales (1 MWel and 6 MWel) were analysed regarding their investment and operation cost. The process chains were based on biological or catalytic bubbling fluidised bed methanation i ...

Synthetic biology aims to engineer cells as miniature biological devices to sense, process, and respond to exogenous stimuli. Protein switches are designed to sense and respond to various molecular queues in a fast and specific manner, which fits the requi ...

We developed an integrated microfluidic cellular processing unit (mCPU) capable of autonomously isolating single cells and performing measurements and on-the-fly analysis of cell- surface dissociation rates, followed by recovery of selected cells. We perfo ...

Thanks to recent advancements in synthetic biology, the dream of creating a synthetic cell has become feasible. However, due to its inherent complexity, one of the fundamental functions of all living systems, i.e., self-replication, remains to be introduce ...

In phenylketonuria, absence or malfunction of the phenylalanine hydroxylase enzyme results in toxic accumulation of phenylalanine in the body. An injectable recombinant enzyme therapy was recently approved and has the potential to improve the quality of li ...

Cell-free systems offer a promising approach to engineer biology since their open nature allows for well-controlled and characterized reaction conditions. In this review, we discuss the history and recent developments in engineering recombinant and crude e ...

Cell-free systems have emerged as a versatile platform for constructing complex biological systems from the bottom-up. In particular, they enable the rapid engineering and characterization of gene regulatory networks, a critical cellular subsystem that all ...

Fatigue safety verification of existing bridges that uses ‘‘re-calculation’’ based on codes, usually results in insufficient fatigue safety, triggering invasive interventions. Instead of “re-calculation”, Structural Health Monitoring (SHM) should be used f ...

Synthetic protein switches are proteins that can be controlled by an external input, and are useful tools to probe protein function. Antibody fragments and antibody mimetics can be selected to bind, activate, or inhibit several diverse protein targets. Nev ...

Recent progress in protein engineering, empowered by the rapidly evolving fields of synthetic biology and DNA synthesis, has enabled the generation of various custom-designed proteins. Their thorough analysis has led to applications in areas including medi ...

Electric buses are particularly suitable for city and suburban routes due to zero local exhaust and noise emissions. The operation schedule interval defines the charging power, bus fleet size and total cost of ownership of a bus. We propose a novel cost-be ...

Infectious diseases and antimicrobial resistance are major burdens in developing countries, where very specific conditions impede the deployment of established medical infrastructures. Since biosensing devices are nowadays very common in developed countrie ...

Protein-mimetic materials are of great interest for biotechnology to grant protein-like properties to artificial systems. Additionally, these materials can be used to shed light on the fundamental properties of proteins in many environments. Nanoparticles, ...

Optogenetic switches are emerging molecular tools for studying cellular processes as they offer higher spatiotemporal and quantitative precision than classical, chemical-based switches. Light-controllable gene expression systems designed to upregulate prot ...

OnePot PURE Cell-Free System

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Advancing Cell-Free Synthetic Biology: From sustained PURE protein synthesis to an isothermal one-pot DNA replication system

Fly-Swat or Cannon? Cost-Effective Language Model Choice via Meta-Modeling

Appropriations and Uses of Travel Time

Improved Cell-Free Transcription-Translation Reactions in Microfluidic Chemostats Augmented with Hydrogel Membranes for Continuous Small Molecule Dialysis

Engineering synthetic adhesins for biophysical investigation and rewiring of host-microbe interactions

Biochemistry of Aminoacyl tRNA Synthetase and tRNAs and Their Engineering for Cell-Free and Synthetic Cell Applications

Techno-Economic Evaluation of Biological and Fluidised-Bed Based Methanation Process Chains for Grid-Ready Biomethane Production

Rational design of protein switches: applications in synthetic biology and cancer immunotherapy

p High-Throughput Single-Cell TCR-pMHC Dissociation Rate Measurements Performed by an Autonomous Microfluidic Cellular Processing Unit

Towards an artificial cell: Development of a system which self-regenerates the protein components of the PURE system in microfluidic reactors

Microfluidics and cell-free synthetic biology in the development of new diagnostic and therapeutic platforms

Bottom-Up Construction of Complex Biomolecular Systems With Cell-Free Synthetic Biology

High-throughput microfluidic platforms for characterizing and engineering cell-free gene regulatory circuits

Quantification of the conditional value of SHM data for the fatigue safety evaluation of a road viaduct

Allosteric Protein Switches based on Antibody Mimetics

Development of microfluidic tools and methods for the biochemical characterization of scFv and Cas9 affinity reagents and their applications in molecular detection

Cost-Benefit Analysis of Electric Bus Fleet with Various Operation Intervals

Assessing the Potential Deployment of Biosensors for Point-of-Care Diagnostics in Developing Countries: Technological, Economic and Regulatory Aspects

Nanoparticle Liposome Interactions

Dual-controlled optogenetic system for the rapid down-regulation of protein levels in mammalian cells