Publication

Label-free second harmonic phase imaging of biological specimen by digital holographic microscopy

Publications associées (29)

Coherent imaging from bacteria to multicellular organisms

Structural and functional imaging of cells, tissues and organisms is crucial for understanding biomedical processes. Fluorescence microscopy is an established tool and has contributed to many discoveries in the life sciences. This technique provides molecu ...

EPFL2017

Intensity and phase fields behind phase-shifting masks studied with high-resolution interference microscopy

Hans Peter Herzig, Myun Sik Kim, Toralf Scharf, Ali Naqavi, Krishnaparvathy Puthankovilakam

We try to find out the details of how light fields behind the structures of photomasks develop in order to determine the best conditions and designs for proximity printing. The parameters that we use approach real situations like structure printing at prox ...

SPIE-Intl Soc Optical Eng2016

Direct Comparison of Second Harmonic Generation and Two-Photon Photoluminescence from Single Connected Gold Nanodimers

Olivier Martin, Jérémy Butet

In this article we compare the two-photon photoluminescence and second harmonic generation from single connected gold nanodimers. Analyzing the particle size-dependent nonlinear optical spectra and performing excitation polarization resolved measurements u ...

American Chemical Society2016

Second-harmonic radiating imaging probes and harmonic holography

Demetri Psaltis, Ye Pu, Ye Pu

Compared with other imaging probes such as fluorescent dyes and quantum dots, second-harmonic radiating imaging probes (SHRIMPs) provide a unique ultrafast, coherent optical contrast that is free of photobleaching and emission intermittency. Using the seco ...

Spie-Int Soc Optical Engineering2016

Lightfields behind amplitude masks: Creating phase discontinuities

Hans Peter Herzig, Wilfried Noell, Myun Sik Kim, Toralf Scharf, Krishnaparvathy Puthankovilakam

Shaping of light fields behind amplitude and phase masks the basis the lithographic structure reproduction. The aerial image or better the intensity distribution defines the resolution of the structure to be printed. Contrast is the main parameter and to a ...

Ieee2016

Gain vs phase in BOTDA setups

Luc Thévenaz, Marcelo Alfonso Soto Hernandez, Alejandro Dominguez Lopez

We evaluate the Brillouin frequency shift (BFS) determination error when using the Brillouin phase spectrum (BPS) instead of the Brillouin gain spectrum (BGS) in BOTDA setups. We compare the error obtained in the BFS determination in both cases, both with ...

Spie-Int Soc Optical Engineering2016

Second-Harmonic Scattering as a Probe of Structural Correlations in Liquids

Sylvie Roke, Michele Ceriotti, Gabriele Tocci, Chung-Wen Liang, David Mark Wilkins

econd-harmonic scattering experiments of water and other bulk molecular liquids have long been assumed to be insensitive to interactions between the molecules. The measured intensity is generally thought to arise from incoherent scattering due to individua ...

Amer Chemical Soc2016

Optical microscopy for imaging through scattering media

Xin Yang

Optical microscopy has been widely used in the life sciences and biological research for several decades. It possesses many advantages over x-ray radiography, computed tomography, ultrasound imaging and magnetic resonance imaging, in terms of being low cos ...

EPFL2014

Digital Phase Reconstruction via Iterative Solutions of Transport-of-Intensity Equation

Michaël Unser, Emrah Bostan, Stamatios Lefkimmiatis, Emmanuel Augustin Julien Froustey

We develop a variational algorithm for reconstructing phase objects from a series of bright field micrographs. Our mathematical model is based on the transport-of-intensity equation (TIE), which links the phase of a complex field to the axial derivative of ...

Ieee2014