Publications associées à Optics Versus Computation: Influence of Illumination and Reconstruction Model Accuracy in Focal-Plane-Scanning Optical Projection Tomography

Publications associées (8)

Optical tweeze-sectioning microscopy for 3D imaging and manipulation of suspended cells

Xiaohao Xu, Olivier Martin, Siarhei Zavatski

Optical manipulation and detection of biological particulates are crucial procedures in biophotonics. Optical sectioning (OS) opens the avenue to three-dimensional (3D) microscopy, but nonoptical approaches, including sample adhesion and mechanical scannin ...

American Association for the Advancement of Science (AAAS)2025

Intelligent and Self-Driving Microscopy of Protein Aggregation in Neurodegenerative Diseases

Khalid Ashraf Mohie Ibrahim

Neurodegenerative diseases, such as Alzheimer's, Parkinson's, and Huntington's, afflict tens of millions of patients worldwide. They are characterized by protein aggregation and progressive neuronal loss, leading to cognitive and motor impairments, and ult ...

EPFL2025

Spatial and stoichiometric in situ analysis of biomolecular oligomerization at single-protein resolution

Maartje Martina Cornelia Bastings

Latest advances in super-resolution microscopy allow the study of subcellular features at the level of single proteins, which could lead to discoveries in fundamental biological processes, specifically in cell signaling mediated by membrane receptors. Desp ...

Springer Science and Business Media LLC2025

Optical imaging of molecules and their dynamics from surfaces to nanoscale confinement

Nathan Cyprien Arsène Ronceray

Molecular behavior can change dramatically at interfaces, and these changes become even more pronounced when molecules are confined within nanoscale dimensions. In living organisms, nanoscale channels play a crucial role in selectively transporting molecul ...

EPFL2024

Using Windowed Radon Transform to Measure Local Coherence Independently of Speed-of-Sound Variations in Plane-Wave Imaging

Jean-Philippe Thiran, Samuel Denys Beuret

Several metrics have been proposed in ultrasound pulse-echo imaging to quantify local coherence, with the coherence factor being the most prominent example. However, the coherence factor does not differentiate losses of coherence caused by variations of sp ...

Institute of Electrical and Electronics Engineers Inc.2024

Exchangeable HaloTag Ligands for Super-Resolution Fluorescence Microscopy

Kai Johnsson, Michelle Susanne Frei

The specific and covalent labeling of the protein HaloTag with fluorescent probes in living cells makes it a powerful tool for bioimaging. However, the irreversible attachment of the probe to HaloTag precludes imaging applications that require transient bi ...

AMER CHEMICAL SOC2023

Focus image scanning microscopy for sharp and gentle super-resolved microscopy

Giorgio Tortarolo

To date, the feasibility of super-resolution microscopy for imaging live and thick samples is still limited. Stimulated emission depletion (STED) microscopy requires high-intensity illumination to achieve sub-diffraction resolution, potentially introducing ...

Nature Portfolio2022

Spatial tissue profiling by imaging-free molecular tomography

Christian Gabriel Schneider, Gioele La Manno, Fabrice David, Halima Hannah Emilie Schede

Several techniques are currently being developed for spatially resolved omics profiling, but each new method requires the setup of specific detection strategies or specialized instrumentation. Here we describe an imaging-free framework to localize high-thr ...

2021