Publications associées à High count rates with total internal reflection fluorescence correlation spectroscopy

Publications associées (23)

Model-Based Estimation of Three-Dimensional Stiffness Parameters in Photonic-Force Microscopy

Michaël Unser, Andrzej Kulik, Philippe Thévenaz, Elena Bertseva

We propose a system to characterize the 3-D diffusion properties of the probing bead trapped by a photonic-force microscope. We follow a model-based approach, where the model of the dynamics of the bead is given by the Langevin equation. Our procedure comb ...

Institute of Electrical and Electronics Engineers2010

Signal strength and integrated intensity in confocal and image scanning microscopy

Giorgio Tortarolo

The properties of signal strength and integrated intensity in a scanned imaging system are reviewed. These prop-erties are especially applied to confocal imaging systems, including image scanning microscopy. The integrated intensity, equal to the image of ...

Optica Publishing Group2023

Comparative study of human erythrocytes by digital holographic microscopy, confocal microscopy, and impedance volume analyzer

Pierre Magistretti, Pierre Marquet, Christian Depeursinge, Benjamin Rappaz

Red blood cell (RBC) parameters such as morphology, volume, refractive index, and hemoglobin content are of great importance for diagnostic purposes. Existing approaches require complicated calibration procedures and robust cell perturbation. As a result, ...

2008

Simple optical nanomotion method for single-bacterium viability and antibiotic response testing

Sandor Kasas, María Inés Villalba

Antibiotic resistance is nowadays a major public health issue. Rapid antimicrobial susceptibility tests (AST) are one of the options to fight this deadly threat. Performing AST with single-cell sensitivity that is rapid, cheap, and widely accessible, is ch ...

National Academy of Sciences2023

Total internal reflection fluorescence correlation spectroscopy (TIR-FCS) with low background and high count-rate per molecule

Theo Lasser, Marcel Leutenegger, Kai Hassler, Ramachandra Rao

We designed a fluorescence correlation spectroscopy (FCS) system for measurements on surfaces. The system consists of an objective-type total internal reflection fluorescence (TIRF) microscopy setup, adapted to measure FCS. Here, the fluorescence exciting ...

2005

Valence Orbitals Driving the Spin Dynamics in a Rare-Earth Single-Atom Magnet

Harald Brune, François Patthey, Stefano Rusponi, Marina Pivetta, Dante Philippe Sblendorio

We combine spin-polarized scanning tunneling microscopy with quantum master equation analysis to investigate the spin dynamics of the single atom magnet Dy on graphene/Ir(111). By performing reading and writing experiments, we show that the strongly spin p ...

AMER PHYSICAL SOC2023

A microfluidic Braille valve platform for on-demand production, combinatorial screening and sorting of chemically distinct droplets

Christoph Merten, Vida Vafaizadeh

Droplet microfluidics is a powerful tool for a variety of biological applications including single-cell genetics, antibody discovery and directed evolution. All these applications make use of genetic libraries, illustrating the difficulty of generating che ...

NATURE PORTFOLIO2022

Gas Separation Membranes with Atom-Thick Nanopores: The Potential of Nanoporous Single-Layer Graphene

Kumar Varoon Agrawal, Luis Francisco Villalobos Vazquez de la Parra, Kuang-Jung Hsu, Deepu Joseph Babu, Cédric Karel J Van Goethem

Gas separation is one of the most important industrial processes and is poised to take a larger role in the transition to renewable energy, e.g., carbon capture and hydrogen purification. Conventional gas separation processes involving cryogenic distillati ...

AMER CHEMICAL SOC2022

Convergent and divergent beam electron holography and reconstruction of adsorbates on free-standing two-dimensional crystals

Tatiana Latychevskaia

Van der Waals heterostructures have been lately intensively studied because they offer a large variety of properties that can be controlled by selecting 2D materials and their sequence in the stack. The exact arrangement of the layers as well as the exact ...

HIGHER EDUCATION PRESS2019

High-quality single crystal diamond diffraction gratings fabricated by crystallographic etching

Olivier Martin, Toralf Scharf, Niels Quack, Christian Santschi, Teodoro Graziosi, Marcell Kristof Kiss, Adrien Marc Toros

We demonstrate a novel method for fabricating single crystal diamond diffraction gratings based on crystallographic etching that yields high-quality diffraction gratings from commercially available diamond plates. Both V-groove and rectangular gratin ...

2019

In Situ Observation of Coulomb Fission of Individual Plasmonic Nanoparticles

Marcel Drabbels, Ulrich Lorenz, Pavel Olshin, Jonathan Mark Voss, Romain Loïc Charbonnier

Reshaping plasmonic nanoparticles with laser pulses has been extensively researched as a tool for tuning their properties. However, in the absence of direct observations of the processes involved, important mechanistic details have remained elusive. Here, ...

2019

An optimized and validated 384-well plate assay to test platelet function in a high-throughput screening format

Nikolaos Stergiopoulos, Olivier Burri, Rodrigo Araujo Fraga Da Silva, Marc Chambon, Augusto Martins Lima, Maiia Bragina, Julien Bortoli Chapalay

Despite significant advances in the treatment of cardiovascular diseases, antiplatelet therapies are still associated with a high risk of hemorrhage. In order to develop new drugs, methods to measure platelet function must be adapted for the high-throughpu ...

TAYLOR & FRANCIS INC2019

Characterization of Ligand Shell for Mixed-Ligand Coated Gold Nanoparticles

Francesco Stellacci, Quy Ong Khac, Zhi Luo

Gold nanoparticles owe a large number of their properties to their ligand shell. Indeed, many researchers routinely use mixtures of ligand molecules for their nanoparticles to impart complex property sets. It has been shown that the morphology of ligand sh ...

American Chemical Society2017

Scanning tunneling microscopy on large bio-molecular systems on surfaces

Sebastian Koslowski

The ever growing demand for the development of new technologies and materials has led to extensive studies inspired by biological functional complexes. Peptides with their outstanding ability to efficiently self-assemble can express a broad spectrum of int ...

EPFL2017

Computer assisted detection of axonal bouton structural plasticity in in vivo time-lapse images

Graham Knott, Anne Alison Jorstad

The ability to measure minute structural changes in neural circuits is essential for long-term in vivo imaging studies. Here, we propose a methodology for detection and measurement of structural changes in axonal boutons imaged with time-lapse two-photon l ...

Elife Sciences Publications Ltd2017

Self-formation of hexagonal nanotemplates for growth of pyramidal quantum dots by metalorganic vapor phase epitaxy on patterned substrates

Elyahou Kapon, Alok Rudra, Benjamin Dwir, Pascal Gallo, Alessandro Surrente, Romain Carron

We demonstrate the self-formation of hexagonal nanotemplates on GaAs (111)B substrates patterned with arrays of inverted tetrahedral pyramids during metal-organic vapor phase epitaxy and its role in producing high-symmetry, site-controlled quantum dots (QD ...

Tsinghua Univ Press2016

Particle clustering phenomena in hot asphalt mixtures with high content of reclaimed asphalt pavements

André-Gilles Dumont, Sara Bressi

One challenge in efficient recycling of asphalt concrete is the adherence of the reclaimed asphalt pavement (RAP) particles or clustering that inhibits fulfilling the aggregate gradation requirements. In order to investigate this phenomenon two types of mi ...

Elsevier Sci Ltd2015

Living-Engineered Valves for Transcatheter Venous Valve Repair

Peter Modregger

Background: Chronic venous insufficiency (CVI) represents a major global health problem with increasing prevalence and morbidity. CVI is due to an incompetence of the venous valves, which causes venous reflux and distal venous hypertension. Several studies ...

2014

Microscopic evidence for strong periodic lattice distortion in two-dimensional charge-density wave systems

Helmuth Berger, Ji Dai

In quasi-two-dimensional electron systems of layered transition metal dichalcogenides (TMDs) there is still controversy about the nature of the transitions to charge-density wave (CDW) phases, i.e., whether they are described by a Peierls-type mechanism or ...

Amer Physical Soc2014

Design and implementation of a fs-resolved transmission electron microscope based on thermionic gun technology

Fabrizio Carbone, Brett Eugene Barwick, Luca Piazza

In this paper, the design and implementation of a femtosecond-resolved ultrafast transmission electron microscope is presented, based on a thermionic gun geometry. Utilizing an additional magnetic lens between the electron acceleration and the nominal cond ...

Elsevier Science Bv2013