Publication

Polarity-Dependent Asymmetric Distribution and MEX-5/6-Mediated Translational Activation of the Era-1 mRNA in C. elegans Embryos

Publications associées (30)

Automated Platform for Long-Term Culture and High-Content Phenotyping of Single C. elegans Worms

Martinus Gijs, Elena Katsyuba, Johan Auwerx, Matteo Cornaglia, Laurent Mouchiroud, Hüseyin Baris Atakan, Rongrong Xiang

The nematode Caenorhabditis elegans is a suitable model organism in drug screening. Traditionally worms are grown on agar plates, posing many challenges for long-term culture and phenotyping of animals under identical conditions. Microfluidics allows for ' ...

Nature Research2019

Aurora A depletion reveals centrosome-independent polarization mechanism in Caenorhabditis elegans

Pierre Gönczy, Coralie Busso, Lukas von Tobel, Christian Gentili, Marie Juliette Ségolène Pierron, Kerstin Klinkert Fabbri, Sarah Herrman

How living systems break symmetry in an organized manner is a fundamental question in biology. In wild-type Caenorhabditis elegans zygotes, symmetry breaking during anterior-posterior axis specification is guided by centrosomes, resulting in anterior-direc ...

2019

Engineered signaling centers for the spatially controlled patterning of human pluripotent stem cells

Matthias Lütolf, Felix Naef, Eric Paquet, Yoji Tabata, Ambroise Roger Vuaridel, François Réal Rivest, Andrea Manfrin

Signaling centers, localized groups of cells that secrete morphogens, play a key role in early development and organogenesis by orchestrating spatial cell fate patterning. Here we present a microfluidic approach that exposes human pluripotent stem cell (hP ...

NATURE PUBLISHING GROUP2019

Quantitative relationships between SMAD dynamics and target gene activation kinetics in single live cells

Felix Naef, Onur Tidin, David Michael Suter, Torgil Elias Friman

The transduction of extracellular signals through signaling pathways that culminate in a transcriptional response is central to many biological processes. However, quantitative relationships between activities of signaling pathway components and transcript ...

Nature Research2019

DPPA2 and DPPA4 are necessary to establish a 2C‐like state in mouse embryonic stem cells

Didier Trono, Julien Léonard Duc, Alexandre Coudray, Alberto De Iaco

After fertilization of the transcriptionally silent oocyte, expression from both parental chromosomes is launched through zygotic genome activation (ZGA), occurring in the mouse at the 2-cell (2C) stage. Among the first elements to be transcribed are the D ...

2019

Memory and relatedness of transcriptional activity in mammalian cell lineages

Felix Naef, David Michael Suter, Aleksandra Mandic, Saeed Omidi Klishami, Nicholas Edward Phillips

Phenotypically identical mammalian cells often display considerable variability in transcript levels of individual genes. How transcriptional activity propagates in cell lineages, and how this varies across genes is poorly understood. Here we combine live- ...

Nature Research2019

The role of OCT4 and SOX2 in regulating chromatin accessibility and cell fate across the cell cycle in embryonic stem cells

Torgil Elias Friman

Precise spatiotemporal regulation of gene expression is essential for development and homeostasis of complex organisms. This is achieved in large part by sequence-specific transcription factors (TF) that bind to genomic regulatory elements to activate or r ...

EPFL2019

Probing the distribution of cortical components, including PtdIns(4,5)P2, during asymmetric division of C. elegans embryos

Melina Jasmin Scholze

Asymmetric cell division is crucial for embryonic development and stem cell lineages. In the one-cell C. elegans embryo, a contractile cortical actomyosin network contributes to asymmetric division by segregating PAR proteins to discrete cortical domains. ...

EPFL2018

8-Channel Single Embryo Pipette For Accurate C-Elegans Bioassays

Thomas Lehnert, Martinus Gijs, Li Dong, Jingwei Zhang

The nematode Caenorhabditis elegans (C. elegans) is a powerful model organism for addressing fundamental biological questions related to human disease and aging. Accurate control of the number of worms is a primary step for quantitative biological or bioch ...

IEEE2018

Lab-on-a-chip technologies for manipulation and imaging of C. elegans worms and embryos

Li Dong

The nematode Caenorhabditis elegans is an attractive model organism, owing notably to its short life cycle, genetic tractability, and optical transparency facilitating microscopic observation. This thesis deals with the realization of technological tools f ...

EPFL2018