TubeSpin bioreactor 50 for the high-density cultivation of Sf-9 insect cells in suspension

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Fluorescence lifetime imaging microscopy (FLIM) is a key technology that provides direct insight into cell metabolism, cell dynamics and protein activity. However, determining the lifetimes of different fluorescent proteins requires the detection of a rela ...

We realized subsurface fluorescence LiDAR with a large format single-photon avalanche diode array capable of localizing and quantifying concentration of fluorescent molecules in heavily scattering media, such as tissue, with submillimeter depth accuracy. ( ...

Fluorescence microscopy is the method of choice to monitor dynamic processes in living cells due to its non-invasive nature. A variety of different fluorophores and labeling systems are currently used to selectively visualise structures or biomolecules of ...

Single-molecule localization microscopy (SMLM) is a powerful method for the imaging of cellular structures. This modality delivers nanoscale resolution by sequentially activating a subset of fluorescent molecules and by extracting their super-resolved posi ...

We designed and engineered a dye production cassette encoding a heterologous pathway, including human tyrosine hydroxylase and Amanita muscaria 4,5-DOPA dioxygenase, for the biosynthesis of the betaxanthin family of plant and fungal pigments in mammalian c ...

Single-molecule localization microscopy (SMLM) describes a family of powerful imaging techniques that dramatically improve spatial resolution over standard, diffraction-limited microscopy techniques and can image biological structures at the molecular scal ...

The development of novel fluorogenic probes and their use in live-cell imaging lead to a plethora of discoveries in biological research. Herein I report on novel red fluorogenic probes for live-cell super-resolution microscopy of various cellular organelle ...

Cell trackers are fluorescent chemical tools that facilitate imaging and tracking cells within live organisms. Despite their versatility, these dyes lack specificity, tend to leak outside of the cell, and stain neighboring cells. Here, we report a dual-act ...

The response of the adaptive immune system is augmented by multimeric presentation of a specific antigen, resembling viral particles. Several vaccines have been designed based on natural or designed protein scaffolds, which exhibited a potent adaptive immu ...

Bimolecular fluorescence complementation (BiFC) was introduced a decade ago as a method to monitor alpha-synuclein (alpha-syn) oligomerization in intact cells. Since then, several alpha-syn BiFC cellular assays and animal models have been developed based o ...

In this study we investigate the occurrence of primary biological aerosol particles (PBAP) over all sectors of the Southern Ocean (SO) based on a 90-day dataset collected during the Antarctic Circumnavigation Expedition (ACE) in austral summer 2016-2017. S ...

Optical microscopy is one widely used tool to study cell functions and the interaction of molecules at a sub-cellular level. Optical microscopy techniques can be broadly divided into two categories: partially coherent and incoherent. Coherent microscopy te ...

Tissue organization is often characterized by specific patterns of cell morphology. How such patterns emerge in developing tissues is a fundamental open question. Here, we investigate the emergence of tissue-scale patterns of cell shape and mechanical tiss ...

Semiconducting single-walled carbon nanotubes (SWCNTs) are among the few photostable optical emitters that are ideal for sensing, imaging, drug delivery, and monitoring of protein activity. These applications often require strategies for immobilizing prote ...

The cell types affected by biological perturbations in complex tissues are uncovered by single-cell analysis. ...

Cellular uptake and intracellular trafficking of polymer conjugates or polymer nanoparticles is typically monitored using fluorescence-based techniques such as confocal microscopy. While these methods have provided a wealth of insight into the internalizat ...

In situ hybridization is used to visualize the spatial distribution of gene transcripts in tissues and in embryos, providing important information about disease and development. Current methods involve the use of complementary riboprobes incorporating non- ...

The application provides fluorescent dyes, which are cyanine dyes that incorporate additional aza moieties in the indolenium heterocycles and/or in the methine chains connecting them. Symmetrical and unsymmetrical chemically reactive azacyanine dyes are de ...

TubeSpin bioreactor 50 for the high-density cultivation of Sf-9 insect cells in suspension

Chattez avec Graph Search

Fluorescence lifetime imaging with a megapixel SPAD camera and neural network lifetime estimation

Single-photon avalanche diode imaging sensor for subsurface fluorescence LiDAR

Photoactivatable Silicon Rhodamines for Super-Resolution Microscopy

Optical diffraction tomography from single-molecule localization microscopy

Genetically encoded betaxanthin-based small-molecular fluorescent reporter for mammalian cells

Single-molecule localization microscopy

Fluorogenic probes for live-cell imaging

Dual-Activatable Cell Tracker for Controlled and Prolonged Single-Cell Labeling

A Nanoscaffolded Spike-RBD Vaccine Provides Protection against SARS-CoV-2 with Minimal Anti-Scaffold Response

Monitoring alpha-synuclein oligomerization and aggregation using bimolecular fluorescence complementation assays: What you see is not always what you get

Sources, Occurrence and Characteristics of Fluorescent Biological Aerosol Particles Measured over the Pristine Southern Ocean

Development of novel experimental and computational methods for three-dimensional coherent and super-resolution microscopy

Self-organized patterning of cell morphology via mechanosensitive feedback

Site-Specific Protein Conjugation onto Fluorescent Single-Walled Carbon Nanotubes

Cell type prioritization in single-cell data

Cellular Uptake and Intracellular Trafficking of Poly(N-(2-Hydroxypropyl) Methacrylamide)

Detection of mRNA by Whole Mount in situ Hybridization and DNA Extraction for Genotyping of Zebrafish Embryos

Azacyanine dyes and use thereof