Publication

Multigene expression in stable CHO cell pools generated with the piggyBac transposon system

Publications associées (28)

Microfluidics and cell-free synthetic biology in the development of new diagnostic and therapeutic platforms

In phenylketonuria, absence or malfunction of the phenylalanine hydroxylase enzyme results in toxic accumulation of phenylalanine in the body. An injectable recombinant enzyme therapy was recently approved and has the potential to improve the quality of li ...

EPFL2021

Considerations of the Impacts of Cell-Specific Growth and Production Rate on Clone Selection-A Simulation Study

Florian Maria Wurm

For the manufacturing of complex biopharmaceuticals using bioreactors with cultivated mammalian cells, high product concentration is an important objective. The phenotype of the cells in a reactor plays an important role. Are clonal cell populations showin ...

2021

The thermally activated distortion with amplification effect and related variant selection in red gold alloys

Cyril Cayron, Roland Logé, Margaux Nathalie Dominique Larcher

Variant selection during the A1→L10 transformation in a polycrystalline red gold alloy close to equiatomic Au-Cu composition has been extensively studied by Electron Backscatter Diffraction (EBSD) in our previous work. The use of a mathematical description ...

2020

Frequency selection in a gravitationally stretched capillary jet in the jetting regime

François Gallaire, Isha Shukla

A capillary jet falling under the effect of gravity continuously stretches while thinning downstream. We report here the effect of external periodic forcing on such a spatially varying jet in the jetting regime. Surprisingly, the optimal forcing frequency ...

Cambridge University Press2020

Efficient viral transduction in mouse inner ear hair cells with utricle injection and AAV9-PHP.B

Bernard Schneider, Paola Andrea Solanes Vega

Viral delivery of exogenous coding sequences into the inner ear has the potential for therapeutic benefit for patients suffering genetic or acquired hearing loss. To devise improved strategies for viral delivery, we investigated two injection techniques, r ...

2020

Transient vitamin B5 starving improves mammalian cell homeostasis and protein production

Nicolas Mermod

Maintaining a metabolic steady state is essential for an organism's fitness and survival when confronted with environmental stress, and metabolic imbalance can be reversed by exposing the organism to fasting. Here, we attempted to apply this physiological ...

ACADEMIC PRESS INC ELSEVIER SCIENCE2020

Linking micro and macroevolution in the presence of migration

Laurent Lehmann, Sophie Myriam Hautphenne, Nicolas Salamin

Understanding macroevolutionary patterns is central to evolutionary biology. This involves the process of divergence within a species, which starts at the microevolutionary level, for instance, when two sub populations evolve towards different phenotypic o ...

ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD2020

Genetically encoded betaxanthin-based small-molecular fluorescent reporter for mammalian cells

Karl Gademann, Simon Sieber, Leo Scheller

We designed and engineered a dye production cassette encoding a heterologous pathway, including human tyrosine hydroxylase and Amanita muscaria 4,5-DOPA dioxygenase, for the biosynthesis of the betaxanthin family of plant and fungal pigments in mammalian c ...

2020