Spatial and stoichiometric in situ analysis of biomolecular oligomerization at single-protein resolution

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Neurodegenerative diseases, such as Alzheimer's, Parkinson's, and Huntington's, afflict tens of millions of patients worldwide. They are characterized by protein aggregation and progressive neuronal loss, leading to cognitive and motor impairments, and ult ...

Proteins drive most cellular functions and are key players in diseases, yet proteomics still lags behind genomics due to the complexity, diversity, and dynamic nature of proteoforms. Nanopore technology – known for real-time, single-molecule DNA sequencing ...

Membrane potential fluctuations have previously been detected using second harmonic (SH) water imaging on neuronal cells and model lipid bilayer membranes. We report that such fluctuations are also visible when membrane potential-sensitive fluorophores are ...

The glucagon-like peptide-1 receptor (GLP-1R), which belongs to the class B1 G protein-coupled receptor (GPCR) family, is an important target for treatment of metabolic disorders, including type 2 diabetes and obesity. The growing interest in GLP-1R-based ...

Optical manipulation and detection of biological particulates are crucial procedures in biophotonics. Optical sectioning (OS) opens the avenue to three-dimensional (3D) microscopy, but nonoptical approaches, including sample adhesion and mechanical scannin ...

Since their discovery in 2012, carbazolyl (iso)phthalonitrile derivatives (Cz(I)PN) have found significant applications as photocatalysts in organic chemistry. Herein, we introduce two efficient methods for incorporating carbazolyl cyanobenzenes into vario ...

Signaling centers are specialized groups of cells that release morphogens, guiding the fate and behavior of adjacent cells during morphogenesis. For instance, the apical-ectodermal-ridge (AER) in developing limbs, is crucial for coordinating cell fate and ...

Signal transmission between neurons requires exocytosis of neurotransmitters from the lumen of synaptic vesicles into the synaptic cleft. Following an influx of Ca2+, this process is facilitated by the Ca2+ sensor synaptotagmin-1. The underlying mechanisms ...

Molecular behavior can change dramatically at interfaces, and these changes become even more pronounced when molecules are confined within nanoscale dimensions. In living organisms, nanoscale channels play a crucial role in selectively transporting molecul ...

Nanomaterial surfaces can be engineered by chemical modifications for the modulation of various surface properties including surface charges, hydrophobicity and chemical reactivity. These properties modulate in turn the interactions between the nanomateria ...

Congenital myasthenic syndrome-22 (CMS22, OMIM 616224) is a rare genetic disorder caused by deleterious genetic variation in the prolyl endopeptidase-like (PREPL) gene. Previous reports have described patients with deletions and nonsense variants in PREPL, ...

The specific and covalent labeling of the protein HaloTag with fluorescent probes in living cells makes it a powerful tool for bioimaging. However, the irreversible attachment of the probe to HaloTag precludes imaging applications that require transient bi ...

Compound structural identification for non-targeted screening of organic molecules in complex mixtures is commonly carried out using liquid chromatography coupled to tandem mass spectrometry (UHPLC-HRMS/MS and related techniques). Instrumental developments ...

ClC-2 transports chloride ions across plasma membranes and plays critical roles in cellular homeostasis. Its dysfunction is involved in diseases including leukodystrophy and primary aldosteronism. AK-42 was recently reported as a specific inhibitor of ClC- ...

Spatial and stoichiometric in situ analysis of biomolecular oligomerization at single-protein resolution

Chattez avec Graph Search

Intelligent and Self-Driving Microscopy of Protein Aggregation in Neurodegenerative Diseases

Advancing nanopore technology toward protein identification and sequencing

Membrane Potential Fluctuations and Water Asymmetry on Plasma Cell and Model Lipid Membranes: Origins, Implications and Properties

Exploring Conformational Transitions in Biased and Balanced Ligand Binding of GLP-1R

Optical tweeze-sectioning microscopy for 3D imaging and manipulation of suspended cells

Peptide‐Carbazolyl Cyanobenzene Conjugates: Enabling Biomolecule Functionalization via Photoredox and Energy Transfer Catalysis

Deciphering the role of signalling centers in limb development and regeneration

Ca2+-dependent lipid preferences shape synaptotagmin-1 C2A and C2B dynamics: Insights from experiments and simulations

Optical imaging of molecules and their dynamics from surfaces to nanoscale confinement

Engineering nanomaterial surfaces for biomedical applications: antiviral surface coatings, antiviral nanoparticles and lipid-based biopharmaceutical drug systems

Missense variants in CMS22 patients reveal that PREPL has both enzymatic and nonenzymatic functions

Exchangeable HaloTag Ligands for Super-Resolution Fluorescence Microscopy

Harnessing data science to improve molecular structure elucidation from tandem mass spectrometry

Cryo-EM structures of ClC-2 chloride channel reveal the blocking mechanism of its specific inhibitor AK-42