Publications associées à Bio-orthogonal Red and Far-Red Fluorogenic Probes for Wash-Free Live-Cell and Super-resolution Microscopy

Publications associées (8)

Mesoscopic light-sheet imaging set-up for 3D SWIR fluorescence intensity and NIR fluorescence lifetime imaging

Fluorescence lifetime imaging offers the unique ability to monitor the molecular status of the tissue microenvironment. However, the scattering nature of tissues remains a challenge for mesoscopic 3D high resolution imaging. We introduce the design of a me ...

SPIE2025

Quantifying Interaction of Single Molecules with Surfaces using Label-Free Microscopy

Jenny Sülzle

Label-free microscopy is a rapidly advancing field that allows for imaging and tracking of objects without the need for (fluorescent) labels. One technique is interferometric scattering (iSCAT) microscopy, which allows the detection and quantification of s ...

EPFL2024

Nanoscale organization of the endogenous ASC speck

Suliana Manley, Christian Sieben

The NLRP3 inflammasome is a central component of the innate immune system. Its activation leads to for-mation of the ASC speck, a supramolecular assembly of the inflammasome adaptor protein ASC. Different models, based on ASC overexpression, have been prop ...

Cell Press2023

Environmentally Sensitive Color-Shifting Fluorophores for Bioimaging

Kai Johnsson, Julien Hiblot

We introduce color-shifting fluorophores that reversibly switch between a green and red fluorescent form through intramolecular spirocyclization. The equilibrium of the spirocyclization is environmentally sensitive and can be directly measured by determini ...

Wiley-VCH Verlag GmbH2020

Evidence for a dual mechanism in the TiO2/CuxO photocatalyst during the degradation of sulfamethazine under solar or visible light: Critical issues

César Pulgarin, Juan Kiwi, Sami Rtimi

This study presents the photocatalytic degradation of sulfamethazine (SMT) on TiO2/CuxO nanotubes (NTs) by a differentiated mechanism under low intensity solar light and indoor visible light irradiation. In the presence of TiO2/CuxO nanotubes, the SMT-degr ...

ELSEVIER SCIENCE SA2019

Azacyanine dyes and use thereof

Riccardo Sinisi, Alma Rosa Morales Morales

The application provides fluorescent dyes, which are cyanine dyes that incorporate additional aza moieties in the indolenium heterocycles and/or in the methine chains connecting them. Symmetrical and unsymmetrical chemically reactive azacyanine dyes are de ...

2018

Microdevice-integrated immunoassays for Point-of-Care Therapeutic Drug Monitoring

Elena-Diana Bojescu

A significant number of drugs exhibit narrow therapeutic windows and high inter-individual pharmacokinetic variability. In such cases, Therapeutic Drug Monitoring (TDM) is compulsory in order to optimize the efficacy of the drug while avoiding adverse effe ...

EPFL2018

A 65k pixel, 150k frames-per-second camera with global gating and micro-lenses suitable for fluorescence lifetime imaging

Edoardo Charbon, Claudio Bruschini, Samuel Burri, Francesco Regazzoni, François Eric Powolny

This paper presents our work on a 65k pixel single-photon avalanche diode (SPAD) based imaging sensor realized in a 0.35 mu m standard CMOS process. At a resolution of 512 by 128 pixels the sensor is read out in 6.4ps to deliver over 150k monochrome frames ...

Spie-Int Soc Optical Engineering2014