Fluorescent Probes for Plasma Membrane Proteins Based on Nile Red

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Plant cells harness osmotic pressures to stiffen their leaves through strong turgor pressures. Key to this osmosisdriven stiffening is the confinement of liquids within semipermeable membranes that can regulate the transport of water molecules and ions. In ...

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Volume electron microscopy is the method of choice for the in situ interrogation of cellular ultrastructure at the nanometer scale, and with the increase in large raw image datasets generated, improving computational strategies for image segmentation and s ...

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The endoplasmic reticulum (ER) extends to the outer (ONM) and the inner (INM) nuclear membrane forming the nuclear envelope (NE) that delimits the nucleoplasm containing the cell genome. Unfolded protein responses (UPRs) and reticulophagy responses increas ...

Recordings of the physiological history of cells provide insights into biological processes, yet obtaining such recordings is a challenge. To address this, we introduce a method to record transient cellular events for later analysis. We designed proteins t ...

Introduction: Glaucoma, the leading cause of irreversible blindness globally, affects more than 70 million people across the world. When initial treatments prove ineffective, especially for cases with high intraocular pressure (IOP), the preferred approach ...

High throughput wide-field second harmonic imaging enables the label-free imaging of interfacial (< 3 nm thick) water, with a spatial resolution of similar to 370 nm using similar to 100 ms acquisition times per image. The obtained interfacial orientationa ...

A gas processing and transport system comprising a natural gas pipeline (1), a plurality of gas conditioning stations (4) connected to the natural gas pipeline between an upstream main natural gas supply and a downstream consumer end, the gas processing st ...

Peroxisomes are eukaryotic organelles that are essential for multiple metabolic pathways, including fatty acid oxidation, degradation of amino acids, and biosynthesis of ether lipids. Consequently, peroxisome dysfunction leads to pediatric-onset neurodegen ...

The 70 kDa heat shock protein (Hsp70) chaperones control protein homeostasis in all ATP-containing cellular compartments. J-domain proteins (JDPs) coevolved with Hsp70s to trigger ATP hydrolysis and catalytically upload various substrate polypeptides in ne ...

Fluorescent Probes for Plasma Membrane Proteins Based on Nile Red

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Osmosis-driven stiffening of structured hydrogels

Subsurface fluorescence time-of-flight imaging using a large-format single-photon avalanche diode sensor for tumor depth assessment

Modular segmentation, spatial analysis and visualization of volume electron microscopy datasets

Resolving bacterial cell biology: from replisome dynamics to cell wall synthesis

Peptide-based virucidal drugs

Control of nuclear envelope dynamics during acute ER stress by LINC complexes disassembly and selective, asymmetric autophagy of the outer nuclear membrane

Recording physiological history of cells with chemical labeling

A new self-adjustable glaucoma valve

Quantified interface chemistry and biophysics via water imaging

Multi-gas processing and transport system

Fluorescent fatty acid conjugates for live cell imaging of peroxisomes

Autorepression of yeast Hsp70 cochaperones by intramolecular interactions involving their J-domains