Publications associées à ASSET: A robust algorithm for the automated segmentation and standardization of early Caenorhabditis elegans embryos

Inheritance of H3K9 methylation regulates genome architecture in Drosophila early embryos

Constitutive heterochromatin is essential for transcriptional silencing and genome integrity. The establishment of constitutive heterochromatin in early embryos and its role in early fruitfly development are unknown. Lysine 9 trimethylation of histone H3 ( ...

Springernature2024

Sperm-contributed centrioles segregate stochastically into blastomeres of 4-cell stage Caenorhabditis elegans embryos

Pierre Gönczy, Fernando Romero Balestra

Whereas both sperm and egg contribute nuclear genetic material to the zygote in metazoan organisms, the inheritance of other cellular constituents is unequal between the 2 gametes. Thus, 2 copies of the centriole are contributed solely by the sperm to the ...

GENETICS SOCIETY AMERICA2023

Dataset for "Extensive programmed centriole elimination unveiled in C. elegans embryos."

Pierre Gönczy, Nils Kalbfuss

Full dataset related to the publication "Extensive programmed centriole elimination unveiled in C. elegans embryos." ...

Zenodo2023

Microfluidic devices for automated analysis of ingested bacteria in the Caenorhabditis elegans intestine

Farzad Rezaeianaran

In this thesis, we explored on-chip high-resolution imaging of the fate of intestinal bacteria and bacterial products in Caenorhabditis elegans (C. elegans). In the first part, we carried out high-resolution z-stack fluorescent imaging of Red Fluorescent P ...

EPFL2023

Extensive programmed centriole elimination unveiled in C. elegans embryos

Pierre Gönczy, Nils Kalbfuss

Centrioles are critical for fundamental cellular processes, including signaling, motility, and division. The extent to which centrioles are present after cell cycle exit in a developing organism is not known. The stereotypical lineage of Caenorhabditis ele ...

2023

Targeted search for scaling genes reveals matrix metalloproteinase 3 as a scaler of the dorsal-ventral pattern in Xenopus laevis embryos

Daria Korotkova

How embryos scale patterning according to size is still not fully understood. Through in silico screening and analysis of reaction-diffusion systems that could be responsible for scaling, we predicted the existence of genes whose expression is sensitive to ...

Cell Press - Elsevier2022

Dissecting centriole fate in C. elegans development

Nils Kalbfuss

The presence of centrioles is critical for fundamental cell and developmental processes, including polarity, migration and division. Although centrioles are present in most proliferating cells, they are thought to vanish after cell cycle exit in some diffe ...

EPFL2022

NMR spectroscopy of a single mammalian early stage embryo

Giovanni Boero, Jürgen Brugger, Marco Grisi, Gaurasundar Marc Conley, Kyle Joel Rodriguez

The resolving power, chemical sensitivity and non-invasive nature of NMR have made it an established technique for in vivo studies of large organisms both for research and clinical applications. NMR would clearly be beneficial for analysis of entities at t ...

Academic Press Inc - Elsevier Science2022

In vitro endoderm emergence and self-organisation in the absence of extraembryonic tissues and embryonic architecture

Stefano Davide Vianello

In humans, mice, and other mammals key internal organs such as the gut, the lungs, the pancreas, and the liver all derive from the same embryonic tissue: the endoderm. The development of all of these structures thus depends on a same set of early cells, an ...

EPFL2022

Physically asymmetric division of the C. elegans zygote ensures invariably successful embryogenesis

Pierre Gönczy, Radek Jankele

Asymmetric divisions that yield daughter cells of different sizes are frequent during early embryogenesis, but the importance of such a physical difference for successful development remains poorly understood. Here, we investigated this question using the ...

2021

KRAB zinc finger protein-mediated control of transposon-embedded regulatory sequences from human germline to early embryos

Alexandra Iouranova

Transposable elements (TEs), also called jumping genes, are genetic elements capable of changing their position within the genome of their host. They make up large fractions of genomes, including 45% of human DNA content, according to current estimates. S ...

EPFL2021

Unequal cleavage of the C. elegans zygote ensures robust embryogenesis

Radek Jankele

Asymmetric cell divisions play key roles in generating cellular diversity during embryogenesis, and in tissue homeostasis during postembryonic life. Particular asymmetric cell divisions producing differentially sized daughter cells were described in many s ...

EPFL2020

Automated phenotyping of Caenorhabditis elegans embryos with a high-throughput-screening microfluidic platform

Martinus Gijs, Matteo Cornaglia, Hüseyin Baris Atakan, Tunç Alkanat, Raphaël Etienne Jean Trouillon

The nematode Caenorhabditis elegans has been extensively used as a model multicellular organism to study the influence of osmotic stress conditions and the toxicity of chemical compounds on developmental and motility-associated phenotypes. However, the sev ...

2020

Patterning and mechanics of somite boundaries in zebrafish embryos

Andrew Charles Oates, Sundar Ram Naganathan

The body axis of vertebrates is subdivided into repetitive compartments called somites, which give rise primarily to the segmented architecture of the musculoskeletal system in the adult body. Somites form in a sequential and rhythmic manner in embryos and ...

2020

In vitro modeling of early mouse development

Mehmet Ugur Girgin

Previous attempts to recapitulate embryogenesis in a developmentally relevant context started with aggregates composed of a few thousand ESCs, termed embryoid bodies (EB), that upon induction of differentiation reveal a surprising level of autonomous cell ...

EPFL2020

Automated Platform for Long-Term Culture and High-Content Phenotyping of Single C. elegans Worms

Martinus Gijs, Elena Katsyuba, Johan Auwerx, Matteo Cornaglia, Laurent Mouchiroud, Hüseyin Baris Atakan, Rongrong Xiang

The nematode Caenorhabditis elegans is a suitable model organism in drug screening. Traditionally worms are grown on agar plates, posing many challenges for long-term culture and phenotyping of animals under identical conditions. Microfluidics allows for ' ...

Nature Research2019

Understanding the Mechanobiology of Early Mammalian Development through Bioengineered Models

Matthias Lütolf, Stefano Davide Vianello

Research in developmental biology has been recently enriched by a multitude of in vitro models recapitulating key milestones of mammalian embryogenesis. These models obviate the challenge posed by the inaccessibility of implanted embryos, multiply experime ...

Cell Press - Elsevier2019

Fractal Analysis And Related Forms Of Complexity Of Embryo Development. Is There A New Tool For Human Embryo Selection?

Maria-Alexandra Paun

A total number of 1092 embryos, originated from 168 couples that underwent IVF cycles, were analyzed in this study. The embryos were cultivated in a continuous culture and monitored using the time lapse system EmbryoScope. The microscopic images of the fer ...

UNIV POLITEHNICA BUCHAREST, SCI BULL2019

DPPA2 and DPPA4 are necessary to establish a 2C‐like state in mouse embryonic stem cells

Didier Trono, Julien Léonard Duc, Alexandre Coudray, Alberto De Iaco

After fertilization of the transcriptionally silent oocyte, expression from both parental chromosomes is launched through zygotic genome activation (ZGA), occurring in the mouse at the 2-cell (2C) stage. Among the first elements to be transcribed are the D ...

2019

DUX is a non-essential synchronizer of zygotic genome activation

Didier Trono, Sonia Verp, Sandra Eloise Kjeldsen, Alberto De Iaco, Delphine Yvette L Grun

Some of the earliest transcripts produced in fertilized human and mouse oocytes code for DUX, a double homeodomain protein that promotes embryonic genome activation (EGA). Deleting Dux by genome editing at the 1- to 2-cell stage in the mouse impairs EGA an ...

2019