Publications associées à In Vivo Molecular Bioluminescence Imaging: New Tools and Applications

Publications associées (23)

Hyperthermic intrathoracic chemotherapy to control malignant pleural mesothelioma

OBJECTIVE: Malignant pleural mesothelioma (MPM) is a rare disease that has a poor response to conventional therapy. Hyperthermic intrathoracic chemotherapy (HITOC), a treatment combining fever-range hyperthermia with intrapleural cisplatin chemotherapy, ha ...

EPFL2023

High sensitivity, incoherent differential imaging

Chiara Bonati

Microscopic visualisation of optically transparent samples has been a topic of interest for several decades. Features such as density or chemical composition can influence the optical phase of transmitted light, and phase contrast can reveal these structur ...

EPFL2022

A ribonucleoprotein transfection strategy for CRISPR/Cas9-mediated gene editing and single cell cloning in rainbow trout cells

Kristin Schirmer

Background The advent of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 technology marked the beginning of a new era in the field of molecular biology, allowing the efficient and precise creation of targeted mutations in the ge ...

2021

Vascular-targeted micelles as a specific MRI contrast agent for molecular imaging of fibrin clots and cancer cells

Anne-Sophie Chauvin

Molecular medical imaging is intended to increase the accuracy of diagnosis, particularly in cardiovascular and cancer-related diseases, where early detection could significantly increase the treatment success rate. In this study, we present mixed micelles ...

ELSEVIER2021

Development of novel tools for imaging of metabolic fluxes in vivo

Tamara Maric

Bioluminescent imaging is a powerful technique that enables imaging in living organisms with high sensitivity, low background signal, low cost and without the need for radioactivity. The emitted photons are produced in the oxidation reaction of luciferin c ...

EPFL2020

Innovative Tools and in vivo Methods for Tuberculosis Drug Discovery and Development

Raphael Christopher Sommer

Worldwide, tuberculosis (TB) is the leading cause of death due to a single infectious agent, claiming 1.6 million human lives and causing >10 million new cases in 2017 alone, mostly in low-income regions. The intracellular pathogen Mycobacterium tuberculos ...

EPFL2019

A new approach to Cas9-based genome editing in Aspergillus niger that is precise, efficient and selectable

Laure Marie Colette Leynaud-Kieffer Curran

Aspergillus niger and other filamentous fungi are widely used in industry, but efficient genetic engineering of these hosts remains nascent. For example, while molecular genetic tools have been developed, including CRISPR/Cas9, facile genome engineering of ...

2019

Bioorthogonal chemistry in bioluminescence imaging

Elena Goun, Aurélien Godinat, Arkadiy Bazhin

Bioorthogonal chemistry has developed significant over the past few decades, to the particular benefit of molecular imaging. Bioluminescence imaging (BLI) along with other imaging modalities have significantly benefitted from this chemistry. Here, we revie ...

ELSEVIER SCI LTD2018

A Biocompatible in Vivo Ligation Reaction and Its Application for Noninvasive Bioluminescent Imaging of Protease Activity in Living Mice

Douglas Hanahan, Aurélien Godinat, Gennady Nikitin, Ke Cheng

The discovery of biocompatible reactions had a tremendous impact on chemical biology, allowing the study of numerous biological processes directly in complex systems. However, despite the fact that multiple biocompatible reactions have been developed in th ...

Amer Chemical Soc2013

The Split Luciferin Reaction : From Bioorthogonal Chemistry to Bioluminescence Imaging

Aurélien Godinat

Studying biological processes on the level of live cells with the help of biocompatible reac-tions has tremendously advanced our understanding of basic biology. However, the great complexity of many human pathologies such as cancer, diabetes and neurodegen ...

EPFL2017

Functional and Structural Imaging with Optical Coherence Microscopy : Principles & Applications

Arno Pino Bouwens

Fluorescence microscopy techniques are well established research tools and have proven their use in a large variety of biomedical applications. Microscopic molecular contrast is achieved by imaging fluorescent dyes that bind specifically to a molecule of i ...

EPFL2013

Visualizing chemical transport from extracellular symbionts into squid host tissues using NanoSIMS

Stéphanie Kéren Cohen

Chemical communication is the basis of host-microbe interaction. Insights into this communication will provide a deeper understanding of the mechanisms that govern these complex associations, in beneficial as well as pathogenic contexts. In this dissertati ...

EPFL2017

ZFN-Site searches genomes for zinc finger nuclease target sites and off-target sites

Philipp Bucher, Giovanna Ambrosini, Christian Iseli

Background: Zinc Finger Nucleases (ZFNs) are man-made restriction enzymes useful for manipulating genomes by cleaving target DNA sequences. ZFNs allow therapeutic gene correction or creation of genetically modified model organisms. ZFN specificity is not a ...

BioMed Central2011

Real-Time Bioluminescence Imaging of Glycans on Live Cells

Cell-surface glycans are attractive targets for molecule imaging due to their reflection of cellular processes associated with development and disease progression. In this paper, we describe the design, synthesis, and biological application of a new phosph ...

American Chemical Society2010

Caged luciferin probes for the bioluminescence imaging of nitroreductase and hydrogen sulfide in living animals

Anzhelika Vorobyeva

Molecular imaging advances our understanding of cellular and molecular functions within complex biological systems. The development of novel imaging probes that can be applied to answer fundamental biological questions, diagnose and monitor disease progres ...

EPFL2016

Bioluminescence for Assessing Drug Potency against Nonreplicating Mycobacterium tuberculosis

Stewart Cole, Neeraj Dhar, Claudia Sala, Anthony Vocat, Ruben Hartkoorn, Ming Zhang, Benoit Lechartier

Targeting dormant Mycobacterium tuberculosis represents a challenge to antituberculosis drug discovery programs. We previously reported and validated the use of the streptomycin (STR)-dependent M. tuberculosis 18b strain as a tool for assessing drug potenc ...

American Society for Microbiology2015

Studies on lentiviral-mediated transgenesis

Marc-Olivier Sauvain

Transgenic animals are essential research tools, whether to address basic biological questions or to develop preclinical models of human diseases. Their generation through the injection of naked plasmid DNA into the male pronucleus of a fertilized oocyte h ...

EPFL2009

Silicon-on-Insulator Technology for Bioluminescence Imaging and Application to a Switching Photodetector

Maher Kayal, Adil Koukab, Denis Sallin

This paper analyzes some advantages of Silicon-on-Insulator (SOI) based photodetectors for bioluminescence imaging. It shows that SOI based sensors not only solve the bulk carriers problem, it can also act as a very selective spectral filter by acting as a ...

Ieee2015

Precursor molecule for the synthesis of d-luciferin

Elena Goun, Aurélien Godinat

The present invention relates to precursor molecules for the synthesis of D-luciferin, or blocked D-luciferin and derivatives thereof, which are functionalized in different positions. Further, the use of functionalized precursor molecules for the synthesis ...

2015

Bioluminescence imaging of small biomolecules

Elena Goun, Aurélien Godinat, Riccardo Sinisi, Grigory Karateev, Ghyslain Martial Alain Budin, Aleksandra Konovalova, Jens Tomas Frigell

The invention relates to a technique to detect small molecules using Bioluminescence imaging (BLI) to image and quantify non-invasively, in vitro and in vivo,intracellular metabolite fluxes and which can be applied to azido-modified compounds, such as azid ...

2014