Publication

3D nanometrology of transparent objects by phase calibration of a basic bright-field microscope for multiple illumination apertures

Publications associées (31)

In Situ and Time-Resolved Transmission Electron Microscopy of Nanoscale Processes

Observing the fast dynamics of nanoscale systems is crucial in order to understand and ultimately control their behavior. Characterizing these dynamic processes requires techniques with atomic spatial resolution and a temporal resolution that matches the t ...

EPFL2023

High sensitivity, incoherent differential imaging

Chiara Bonati

Microscopic visualisation of optically transparent samples has been a topic of interest for several decades. Features such as density or chemical composition can influence the optical phase of transmitted light, and phase contrast can reveal these structur ...

EPFL2022

Advanced Techniques in Optical Diffraction Tomography

Ahmed Bassam Sayed Ayoub Mohamed Emam

In this thesis, we study the 3 challenges described above. First, we study different reconstruction techniques and assess the fidelity of each reconstruction results by means of structured illumination and phase conjugation. By reconstructing the 3D refrac ...

EPFL2022

Phase Reconstruction of Low-Energy Electron Holograms of Individual Proteins

Klaus Kern, Stephan Rauschenbach, Sven Alexander Szilagyi, Hannah Julia Ochner

Low-energy electron holography (LEEH) is one of the few techniques capable of imaging large and complex three-dimensional molecules, such as proteins, on the single molecule level at subnanometer resolution. During the imaging process, the structural infor ...

AMER CHEMICAL SOC2022

Nonlinear Inverse Problems in Quantitative Phase Imaging

Thanh-An Michel Pham

The topic of this thesis is the development of new algorithmic reconstruction methods for quantitative phase imaging (QPI). In the past decade, advanced QPI has emerged as a valuable tool to study label-free biological samples and uncover their 3D structur ...

EPFL2022

Stain-free identification of cell nuclei using tomographic phase microscopy in flow cytometry

Demetri Psaltis, Joowon Lim, Daniele Pirone

The accurate identification of the three-dimensional quantitative shape of a cell nucleus is now possible without fluorescent staining by applying computational segmentation to refractive index tomograms recorded in the flow cytometry mode. ...

Nature Publishing Group2022

Optical microscopy gets down to angstroms

Suliana Manley, Giorgio Tortarolo

Super-resolution microscopy at angstrom precision could pave the way to optical structural biology in cells. ...

NATURE PORTFOLIO2022

Application of Copper Thermal Spraying for Electrical Joints between Superconducting Nb3Sn Cables

Pierluigi Bruzzone, Vincenzo D'Auria

This manuscript reports on the application of copper thermal spraying in the manufacturing process of an electrical connection between Nb3Sn cables for superconducting magnets of fusion reactors. The joint is realized through diffusion bonding of the spray ...

MDPI2022

Method and light microscope with a plurality of arrays of photon-counting detector elements

Claudio Bruschini, Edoardo Charbon, Ivan Michel Antolovic

A method for operating a light microscope comprises emitting and guiding a plurality of illumination light beams towards a specimen (6) to form a plurality of separated illumination light spots (2A, 2B, 2C, 2D) at the specimen; and guiding detection light ...

2022

Lock-in amplified differential phase contrast

Christophe Moser, Damien Claude-Marie Loterie, Timothé Laforest, Chiara Bonati

We introduce a lock-in method to increase the phase contrast in incoherent Differential Phase Contrast (DPC) imaging. The use of a smart pixel detector with in-pixel signal demodulation, paired with synchronized illumination, provides the basis of a bit-ef ...

SPIE-INT SOC OPTICAL ENGINEERING2022

A Multifractal Vision of 5-Fluorouracil Release from Chitosan-Based Matrix

Maria-Alexandra Paun

A suite of four drug deliverance formulations grounded on 5-fluorouracil enclosed in a chitosan-founded intercellular substance was produced by 3,7-dimethyl-2,6-octadienal with in situ hydrogelation. The formulations have been examined from a morphological ...

MDPI2022