Genetic targeting of chemical indicators in vivo

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Single-molecule localization microscopy (SMLM) describes a family of powerful imaging techniques that dramatically improve spatial resolution over standard, diffraction-limited microscopy techniques and can image biological structures at the molecular scal ...

Background The advent of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 technology marked the beginning of a new era in the field of molecular biology, allowing the efficient and precise creation of targeted mutations in the ge ...

It is difficult to develop suitable fluorescent probes for live-cell nanoscopy, but a general strategy is now reported that can transform regular fluorophores into fluorogenic probes with excellent cell permeability and low unspecific background signals. U ...

Fluorescence lifetime imaging (FLI) is increasingly recognized as a powerful tool for biochemical and cellular investigations, including in vivo applications. Fluorescence lifetime is an intrinsic characteristic of any fluorescent dye which, to a large ext ...

Conventional therapeutics are often limited by their targeting ability, resulting in harmful and potential fatal side-effects for the patients. Recently, new strategies have been developed to improve target specificity of drugs in order to generate more ef ...

Studying dynamic biological processes, such as heart development and function in zebrafish embryos, often relies on multi-channel fluorescence labeling to distinguish multiple anatomical features, yet also demands high frame rates to capture rapid cell mot ...

Fluorescence microscopy is the method of choice to monitor dynamic processes in living cells due to its non-invasive nature. A variety of different fluorophores and labeling systems are currently used to selectively visualise structures or biomolecules of ...

We designed and engineered a dye production cassette encoding a heterologous pathway, including human tyrosine hydroxylase and Amanita muscaria 4,5-DOPA dioxygenase, for the biosynthesis of the betaxanthin family of plant and fungal pigments in mammalian c ...

DNA-protein interactions lie at the crux of life's essential processes. As such, various technologies have been developed to characterize these interactions. The distinct advantages of these technologies can be leveraged to study different facets of these ...

Caenorhabditis elegans(C. elegans) constitutes an important model organism for use in nutrition and aging studies. We report a novel method for studying the dynamics ofEscherichia coli(E. coli) bacterial transit through the worms' intestine. A microfluidic ...

Fluorescence lifetime imaging microscopy (FLIM) is a key technology that provides direct insight into cell metabolism, cell dynamics and protein activity. However, determining the lifetimes of different fluorescent proteins requires the detection of a rela ...

Semiconducting single-walled carbon nanotubes (SWCNTs) are among the few photostable optical emitters that are ideal for sensing, imaging, drug delivery, and monitoring of protein activity. These applications often require strategies for immobilizing prote ...

Genetic targeting of chemical indicators in vivo

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Single-molecule localization microscopy

A ribonucleoprotein transfection strategy for CRISPR/Cas9-mediated gene editing and single cell cloning in rainbow trout cells

A general strategy to develop cell permeable and fluorogenic probes for multicolour nanoscopy

Single-Photon, Time-Gated, Phasor-Based Fluorescence Lifetime Imaging through Highly Scattering Medium

Chemistry and Analysis of Nanoparticle-Modified Living Cells

Temporal resolution doubling in fluorescence light-sheet microscopy via a hue-encoded shutter and regularization

Photoactivatable Silicon Rhodamines for Super-Resolution Microscopy

Genetically encoded betaxanthin-based small-molecular fluorescent reporter for mammalian cells

Establishing a Ternary System for Optical Monitoring of DNA-Protein Interactions with Single-Walled Carbon Nanotubes

Anin vivomicrofluidic study of bacterial transit inC. elegansnematodes

Fluorescence lifetime imaging with a megapixel SPAD camera and neural network lifetime estimation

Site-Specific Protein Conjugation onto Fluorescent Single-Walled Carbon Nanotubes