Publications associées à Reconstruction in interferometric synthetic aperture microscopy: comparison with optical coherence tomography and digital holographic microscopy

3D diffractive optics for linear interconnects and nonlinear processing

The optical domain presents potential avenues for enhancing both computing and communication due to its inherent properties of bandwidth, parallelism, and energy efficiency. This research focuses on harnessing 3-Dimensional (3D) diffractive optics for nove ...

EPFL2024

Subsurface fluorescence time-of-flight imaging using a large-format single-photon avalanche diode sensor for tumor depth assessment

Claudio Bruschini, Edoardo Charbon, Arin Can Ülkü, Yichen Feng

Significance: Fluorescence guidance is used clinically by surgeons to visualize anatomical and/or physiological phenomena in the surgical field that are difficult or impossible to detect by the naked eye. Such phenomena include tissue perfusion or molecula ...

SPIE Society of Photo-optical Instrumentation Engineers2024

Practical computational imaging by use of spatiotemporal light modulation: from simulations to applications in biological microscopy

François Thierry M Marelli

Optical microscopy is an essential tool for biologists, who are often faced with the need to overcome the spatial and temporal resolution limitations of their devices to capture finer details. As upgrading imaging hardware is expensive, computational metho ...

EPFL2023

Advanced Techniques in Optical Diffraction Tomography

Ahmed Bassam Sayed Ayoub Mohamed Emam

In this thesis, we study the 3 challenges described above. First, we study different reconstruction techniques and assess the fidelity of each reconstruction results by means of structured illumination and phase conjugation. By reconstructing the 3D refrac ...

EPFL2022

Optical Diffraction Tomography Using Nearly In-Line Holography with a Broadband LED Source

Demetri Psaltis, Ahmed Ayoub, Abhijit Roy

We present optical tomography methods for a 3D refractive index reconstruction of weakly scattering objects using LED light sources. We are able to record holograms by minimizing the optical path difference between the signal and reference beams while sepa ...

MDPI2022

High sensitivity, incoherent differential imaging

Chiara Bonati

Microscopic visualisation of optically transparent samples has been a topic of interest for several decades. Features such as density or chemical composition can influence the optical phase of transmitted light, and phase contrast can reveal these structur ...

EPFL2022

Nonlinear Inverse Problems in Quantitative Phase Imaging

Thanh-An Michel Pham

The topic of this thesis is the development of new algorithmic reconstruction methods for quantitative phase imaging (QPI). In the past decade, advanced QPI has emerged as a valuable tool to study label-free biological samples and uncover their 3D structur ...

EPFL2022

Polarization-sensitive optics diffraction tomography

Demetri Psaltis, Ahmed Ayoub, Joowon Lim, Elizabeth Elena Antoine, Amirhossein Saba Shirvan

Polarization of light has been widely used as a contrast mechanism in two-dimensional (2D) microscopy and also in some three-dimensional (3D) imaging modalities. In this paper, we report the 3D tomographic reconstruction of the refractive index (RI) tensor ...

2021

Phase calibration of a basic bright-field microscope for 3D metrology of transparent samples at the nanoscale

Martinus Gijs, Daniel Migliozzi, Bingying Zhao

Optical retrieval of the structure of transparent objects at the nano-scale requires adapted methods capable of probing their interaction with suitable light. Scattering events, for instance, depend on the content and the arrangement of the medium encounte ...

SPIE-INT SOC OPTICAL ENGINEERING2021

High-resolution interference microscopy with spectral resolution for the characterization of individual particles and self-assembled meta-atoms

Toralf Scharf, Michail Symeonidis, Andreas Vetter

We apply a high-resolution interference microscope with spectral resolution to investigate the scattering response of isolated meta-atoms in real space. The final meta-atoms consist of core-shell clusters that are fabricated using a bottom-up approach. The ...

2019

Biomedical optical techniques for intracochlear cellular imaging

Marilisa Romito

Imaging the inner ear microanatomy is of great importance for the assessment of the state of the cells responsible for sound detection. Hearing disorders are mainly due to a malfunction of the cochlea, the bone containing the cells inside the inner ear. Un ...

EPFL2019

Phase resolved characterization of nanoscale features in optical microstructures

Michail Symeonidis

In this study, we apply interferometric microscopy to study the phase, alongside the intensity, of the light field transmitted through a wide variety of samples. Additionally, we conduct those interferometric measurements at different wavelengths within th ...

EPFL2019

Label-free, scalable and point-of-care imaging platform for rapid analysis of biomarker

Hatice Altug, Alexander Belushkin, Filiz Yesilköy, Yasaman Jahani

We propose a disruptive point-of-care (PoC) imaging platform based on lens-free interference phase-contrast imaging for rapid detection of biomarker such as for sepsis and potentially other diseases (e.g. cancer). It enables simultaneous analysis of potent ...

SPIE-INT SOC OPTICAL ENGINEERING2019

Optical projection tomography for whole organ imaging

David Nguyen

In the past twenty years, far-reaching studies of molecular and cellular processes have reached a milestone in their maturation, and the knowledge from these studies was ready to apply at higher organizational levels. At that time, rodent models were long ...

EPFL2019

Reconstruction of image sequences from ungated and scanning-aberrated laser scanning microscopy images of the beating heart

Michael Stefan Daniel Liebling, Olivia Mariani

Fluorescence laser-scanning microscopy is a well-established imaging technique in biology, available in many imaging facilities to investigate structures within live animal embryos such as zebrafish. Laser scanning microscopes (LSM) are limited when used t ...

2020

3D Reconstruction of Optical Diffraction Tomography Based on a Neural Network Model

Morteza Hasani Shoreh

Optical tomography has been widely investigated for biomedical imaging applications. In recent years, it has been combined with digital holography and has been employed to produce high quality images of phase objects such as cells. In this Thesis, we look ...

EPFL2018

Structural and functional brain imaging using extended-focus optical coherence tomography and microscopy

Paul James Marchand

Neuroimaging techniques aim at revealing the anatomy and functional organisation of cerebral structures. Over the past decades, functional magnetic resonance imaging (fMRI) has revolutionized our understanding of human cerebral physiology through its abili ...

EPFL2018

Compact lensless digital holography

Manon Rostykus

Microscopy is of high interest for biology since it allows imaging features that are too small to be seen with naked eyes. However, cells are mostly transparent to visible and infrared light which makes it difficult to see with a traditional microscope. To ...

EPFL2018

Tomographic imaging with harmonic holography in tissues of known refractive index distribution

Demetri Psaltis, Ye Pu

High-resolution imaging in thick biological tissues beyond a few transport mean free path of photons remains a formidable challenge to modern science and technology. Recent advancements in optical tomography have enabled the reconstruction of the three-dim ...

SPIE-INT SOC OPTICAL ENGINEERING2018

Learning Tomography Assessed Using Mie Theory

Michaël Unser, Demetri Psaltis, Joowon Lim

In optical diffraction tomography, the multiply scattered field is a nonlinear function of the refractive index (RI) of the object. The Rytov method relies on a single-scattering propagation model and is commonly used to reconstruct images. Recently, a rec ...

American Physical Society (APS)2018