A microfluidic array for high-content screening at whole-organism resolution

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Aminopeptidases, such as dipeptidyl peptidase 4 (DPP-4, CD26), are potent therapeutic targets for pharmacological interventions because they play key roles in many important pathological pathways. To analyze amino-peptidase activity in vitro (including hig ...

Despite significant advances in the treatment of cardiovascular diseases, antiplatelet therapies are still associated with a high risk of hemorrhage. In order to develop new drugs, methods to measure platelet function must be adapted for the high-throughpu ...

C. elegans is an attractive model organism in biology, as it shows genetic similarity with humans, facilitates microscopic observation due to its transparency, and has a short life cycle. Moreover, many mutants expressing fluorescent proteins in particular ...

C. elegans is frequently studied to address fundamental biological questions motivated by its short life cycle, small size, hermaphrodite behavior and well-annotated genome. In this work, we present novel microfluidic platforms that support long-term cultu ...

Tandem ion mobility mass spectrometry-coupled laser excitation is used to record photodetachment, photoisomerization and photodepletion action spectra for a series of deprotomer-selected hydroxycinnamate anions, including deprotonated caffeic, ferulic and ...

Increasing interest in understanding fundamental biological processes, among which aging and nutrition, has led to the study of simple and powerful model organisms, as the round-worm Caenorhabditis elegans (C. elegans). The nematode, selected by Sydney Bre ...

Caenorhabditis elegans is a performant model system for studying human biological processes and diseases and for pre-clinical phenotyping screenings of compounds. Microfluidics has been instrumental in enabling C. elegans-based drug assays, facilitating th ...

Colorectal cancer (CRC) is one of the deadliest cancers worldwide. Despite extensive research in the field, new therapies targeting CRC are in urgent need. CRC is a highly heterogeneous disease with high levels of intra- and inter-patient heterogeneity. Ca ...

Nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucleotide phosphate (NADP) are crucial redox cofactors regulating energy metabolism, reductive biosynthesis and the antioxidant defense system. Besides their role as redox cofactors, NAD(P ...

This paper presents a dedicated study of plasma-antenna (PA) coupling with the Alfven Eigenmode Active Diagnostic (AEAD) in JET. Stable AEs and their resonant frequencies f, damping rates gamma < 0, and toroidal mode numbers n are measured for various PA s ...

In a typical high-throughput drug screening (HTS) process, up to millions of chemical compounds are applied to cells cultured in well plates, aiming to find molecules that exhibit a robust dose-response, as evidenced for example by a fluorescence signal. I ...

We have induced hyperpolarized long-lived states in compounds containing C-13-bearing methyl groups by dynamic nuclear polarization (DNP) at cryogenic temperatures, followed by dissolution with a warm solvent. The hyper polarized methyl long-lived states g ...

Bioprinting of functional tissues could overcome tissue shortages and allow a more rapid response for treatments. However, despite recent progress in bioprinting, and its outstanding ability to position cells and biomaterials in a precise 3D manner, its su ...

With the advent of high-throughput and genome-wide screening initiatives, there is a need for improved methods for cell-based assays. Current approaches require expensive equipment, rely on large-scale culturing formats not suited for small or rare sample ...

The form and structure of biological tissues define their function. The emergence of tissue morphology during development is one of the wonders of nature. Cells mechanically probe and manipulate their surroundings while constructing structures from the ext ...

The present manuscript documents the development of a practical calculation scheme to model the response of a diamond detector in the mixed (neutron and gamma) radiation field of the CROCUS experimental reactor at EPFL. The model is shown to perform reason ...

PyMOL, a cross-platform molecular graphics tool, has been widely used for three-dimensional (3D) visualization of proteins, nucleic acids, small molecules, electron densities, surfaces, and trajectories. It is also capable of editing molecules, ray tracing ...

A microfluidic array for high-content screening at whole-organism resolution

Chattez avec Graph Search

A Universal Assay for Aminopeptidase Activity and Its Application for Dipeptidyl Peptidase-4 Drug Discovery

An optimized and validated 384-well plate assay to test platelet function in a high-throughput screening format

Studying the roundworm Caenorhabditis elegans using microfluidic chips

Long-term imaging, automated high-content phenotyping and sorting of C. elegans larvae and embryos using microfluidic platforms

Action spectroscopy of deprotomer-selected hydroxycinnamate anions

Microfluidic culture platforms for C. elegans bacterial interaction and digestion studies at single-organism resolution

Microfluidic high-content phenotypic screening on a Caenorhabditis elegans model

Intestinal stem cells differentiation and its application in cancer therapy

Semisynthetic Biosensors for the Quantification of Nicotinamide Adenine Dinucleotides in Live Cells

Experimental studies of plasma-antenna coupling with the JET Alfven Eigenmode Active Diagnostic

Microfluidic systems for high-throughput and high-content screening using the nematode Caenorhabditis elegans

Dynamic Nuclear Polarization of Long-Lived Nuclear Spin States in Methyl Groups

Bioprinting of Cartilaginous Auricular Constructs Utilizing an Enzymatically Crosslinkable Bioink

Microfluidic platforms for high-throughput mammalian cell printing, transfection, and dosage-dependent studies

Mechanical principles of engineering fibrous tissues with programmable shape

Modeling the response of a diamond detector in the zero power reactor CROCUS

Using PyMOL as a platform for computational drug design