Microfluidic system for Caenorhabditis elegans culture and oxygen consumption rate measurements

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Long-term imaging, automated high-content phenotyping and sorting of C. elegans larvae and embryos using microfluidic platforms

Hüseyin Baris Atakan

C. elegans is frequently studied to address fundamental biological questions motivated by its short life cycle, small size, hermaphrodite behavior and well-annotated genome. In this work, we present novel microfluidic platforms that support long-term cultu ...

EPFL2019

Studying the roundworm Caenorhabditis elegans using microfluidic chips

Martinus Adela Maria Gijs

C. elegans is an attractive model organism in biology, as it shows genetic similarity with humans, facilitates microscopic observation due to its transparency, and has a short life cycle. Moreover, many mutants expressing fluorescent proteins in particular ...

SPIE-INT SOC OPTICAL ENGINEERING2019

System for capture or immunoprecipitation of a protein-dna complex

Bart Deplancke, Riccardo Dainese

The present invention concerns a system for immunoprecipitation of a protein-DNA complex or for capture of a protein-DNA complex including: - a microfluidic device including a plurality of antibody units, wherein each antibody unit includes at least one mi ...

2019

Integrated microfluidic tools for the characterization of protein/DNA interactions in vitro and in vivo

Riccardo Dainese

The specific interaction between DNA and proteins constitutes one of the crucial elements in the regulation of gene expression. This thesis focuses on the development and optimization of two microfluidic-based technologies called SMiLE-seq and FloChIP that ...

EPFL2019

MicroRNA-382 silencing induces a mitonuclear protein imbalance and activates the mitochondrial unfolded protein response in muscle cells

Johan Auwerx, Norman Lionel Moullan, Xu Wang, Pénélope Andreux

Proper mitochondrial function plays a central role in cellular metabolism. Various diseases as well as aging are associated with diminished mitochondrial function. Previously, we identified 19 miRNAs putatively involved in the regulation of mitochondrial m ...

2019

Mitochondrial oxidative capacity and NAD(+) biosynthesis are reduced in human sarcopenia across ethnicities

Patrick Descombes, Tanja Sonntag

The causes of impaired skeletal muscle mass and strength during aging are well-studied in healthy populations. Less is known on pathological age-related muscle wasting and weakness termed sarcopenia, which directly impacts physical autonomy and survival. H ...

Springer2019

Label-free plasmonic biosensors for real-time live cell analysis

Xiaokang Li

For decades, the understanding of life has been focused on its most fundamental building blocks: molecules. Deciphering the dynamic activities of protein molecules in human cells is of vital significance for fundamental and clinical studies. The minuscule ...

EPFL2019

Mitochondrial haplotypes affect metabolic phenotypes in the Drosophila Genetic Reference Panel

Johan Auwerx, Bart Deplancke, Adamantia Kapopoulou, Maria Litovchenko, Brian Dennis Hollis, Roel Paulus Josephus Bevers, Virginie Sandra Braman

The nature and extent of mitochondrial DNA variation in a population and how it affects traits is poorly understood. Here we resequence the mitochondrial genomes of 169 Drosophila Genetic Reference Panel lines, identifying 231 variants that stratify along ...

2019

Microfluidic high-content phenotypic screening on a Caenorhabditis elegans model

Maria Cristina Letizia

Caenorhabditis elegans is a performant model system for studying human biological processes and diseases and for pre-clinical phenotyping screenings of compounds. Microfluidics has been instrumental in enabling C. elegans-based drug assays, facilitating th ...

EPFL2018

Medium chain triglyceride diet reduces anxiety-like behaviors and enhances social competitiveness in rats

Maria del Carmen Sandi Perez, Carlos Canto Alvarez, Fiona Hollis

Medium-chain triglycerides (MCT) are emerging as unique dietary supplements that are potentially relevant for the amelioration of brain dysfunctions. MCT are converted into ketones and free medium chain fatty acids that, in the brain, are highly effective ...

2018

Capsules with submicron shells: production, stability, and leakage behavior

Antoine Claude Jean Vian

Capsules are often employed to prolong the shelf-life of active ingredients such as many drugs, food additives, or cosmetic substances because they delay their oxidation or prevent interactions with molecules contained in the surrounding. If designed prope ...

EPFL2018

Quantifying and Localizing the Mitochondrial Proteome Across Five Tissues in A Mouse Population

Johan Auwerx, Dongryeol Ryu, Jun Yong Kim, Pooja Jha, Evan Graehl Williams

We have used SWATH mass spectrometry to quantify 3648 proteins across 76 proteomes collected from genetically diverse BXD mouse strains in two fractions (mitochondria and total cell) from five tissues: liver, quadriceps, heart, brain, and brown adipose (BA ...

AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC2018