Publications associées à Specific Stimulation and Multiplex Secretion Analysis of T Cells Using a Suspendable Hydrogel Platform Enables Immunophenotyping at the Single-Cell Scale

Publications associées (14)

Engineering a synthetic signaling network in dendritic cells to improve anti-tumoral immunity

Effective induction of anti-tumor immunity requires dendritic cells (DCs) to internalize, process and present tumor antigens to T cells. However, the hostile tumor microenvironment (TME) impedes this process by suppressing DC maturation and antigen present ...

EPFL2024

Non-coding variants impact cis-regulatory coordination in a cell type-specific manner

Bart Deplancke, Vincent Roland Julien Gardeux, Guido Van Mierlo, Wouter Jozef L Saelens, Olga Pushkarev, Judith Franziska Kribelbauer

Background: Interactions among cis-regulatory elements (CREs) play a crucial role in gene regulation. Various approaches have been developed to map these interactions genome-wide, including those relying on interindividual epigenomic variation to identify ...

2024

Vaccine breakthrough hypoxemic COVID-19 pneumonia in patients with auto-Abs neutralizing type I IFNs

Jacques Fellay

Life-threatening "breakthrough"cases of critical COVID-19 are attributed to poor or waning antibody (Ab) response to SARS-CoV-2 vaccines in individuals already at risk. Preexisting auto-Abs neutralizing type I IFNs underlie at least 15% of critical COVID-1 ...

2023

Sphingolipids accumulate in aged muscle, and their reduction counteracts sarcopenia

Johan Auwerx, Maroun Bou Sleiman, Davide D'Amico, Tanes Imamura de Lima, Pirkka-Pekka Untamo Laurila, Martin Rainer Wohlwend, Peiling Luan, Qi Wang, Sébastien Robert Victor Herzig

Age-related muscle dysfunction and sarcopenia are major causes of physical incapacitation in older adults and currently lack viable treatment strategies. Here we find that sphingolipids accumulate in mouse skeletal muscle upon aging and that both genetic a ...

2022

Biotin-NeutrAvidin Mediated Immobilization of Polymer Micro- and Nanoparticles on T Lymphocytes

Harm-Anton Klok, Olivier Burri, Romain Guiet, Arne Seitz, Maxime Ayer

Cells are powerful carriers that can help to improve the delivery of nanomedicines. One approach to use cells as carriers is to immobilize the nanoparticulate cargo on the cell surface. While a plethora of chemical conjugation strategies are available to b ...

2021

Targeted protein degradation through cytosolic delivery of monobody binders using bacterial toxins

Oliver Hantschel, Nadine Eliane Schmit, Katyayanee Neopane

Monobodies are small engineered binding proteins that, upon expression in cells, can inhibit signaling of cytosolic oncoproteins with outstanding selectivity. Efficacy may be further increased by inducing degradation of monobody targets through fusion to t ...

2019

Microfluidic-based immunohistochemistry for breast cancer diagnosis: a comparative clinical study

Fabio Aimi, Martinus Adela Maria Gijs, Diego Gabriel Dupouy, Saska Brajkovic

Breast cancer is a highly heterogeneous disease. The efficacy of tailored therapeutic strategies relies on the precise detection of diagnostic biomarkers by immunohistochemistry (IHC). Therefore, considering the increasing incidence of breast cancer cases, ...

2019

EspL is essential for virulence and stabilizes EspE, EspF and EspH levels in Mycobacterium tuberculosis

Sofia Ulrika Magnusdotter Von Schultz, Stewart Cole, Claudia Sala, Andrej Benjak, Nina Theres Odermatt, Paloma Julia Soler Arnedo

The ESX-1, type VII, secretion system represents the major virulence determinant of Mycobacterium tuberculosis, one of the most successful intracellular pathogens. Here, by combining genetic and high-throughput approaches, we show that EspL, a protein of 1 ...

PUBLIC LIBRARY SCIENCE2018

The effects of mitochondrial- and histone stress on the C. elegans transcriptome

Johan Auwerx, Maroun Bou Sleiman, Pedro Moral Quiros, Olli Juhani Matilainen

The aim of this study was to explore whether mitochondrial- and histone stress cause any common changes in global gene expression. For this experiment, we performed RNA sequencing from control RNAi, cco-1 RNAi (mitochondrial stress) and his-3 RNAi (histone ...

EPFL Infoscience2017

A balancing act between stratification and EMT in cultured human thymic epithelial cells

Matteo Pluchinotta

The thymus is the primary organ for T cell differentiation and maturation. Its stroma forms a characteristic sponge-like 3D structure mainly composed of thymic epithelial cells. Despite of this unconventional epithelial architecture, TECs express markers a ...

EPFL2016

Mechanical and Functional Study of Cell Physiology at the Nanoscale

Pascal Damian Odermatt

By live-cell imaging of biological samples dynamic cellular processes can be resolved. Fluorescence microscopy (FM) and atomic force microscopy (AFM) are both capable of imaging live cells. By combining these techniques structural as well as functional inf ...

EPFL2016

Enhancing Efficacy of Anticancer Vaccines by Targeted Delivery to Tumor-Draining Lymph Nodes

Jeffrey Alan Hubbell, Melody Swartz, Alexandre de Titta, Pedro Romero, Marie Ballester, Laura Jeanbart, Patricia Corthésy Henrioud

The sentinel or tumor-draining lymph node (tdLN) serves as a metastatic niche for many solid tumors and is altered via tumor-derived factors that support tumor progression and metastasis. tdLNs are often removed surgically, and therapeutic vaccines against ...

Amer Assoc Cancer Research2014

DNA Transport across the Outer and Inner Membranes of Naturally Transformable Vibrio cholerae Is Spatially but Not Temporally Coupled

Melanie Blokesch, Patrick Martin Seitz

The physiological state of natural competence for transformation allows certain bacteria to take up free DNA from the environment and to recombine such newly acquired DNA into their chromosomes. However, even though conserved components that are required t ...

American Society for Microbiology2014

Exploring new therapeutic strategies for Huntington's Disease

Wanqiu Hu

Huntington’s disease (HD) is an inherited neurodegenerative disorder caused by an extended poly-glutamine tract in the huntingtin gene (HTT). Despite intensive efforts in understanding its pathogenesis over the decades, effective treatments for HD remain u ...

EPFL2014