Scanning Electrochemical Microscopy as a Readout Tool for Protein Electrophoresis

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Purpose: This study aims to evaluate two distinct approaches for fiber radius estimation using diffusion-relaxation MRI data acquired in biomimetic microfiber phantoms that mimic hollow axons. The methods considered are the spherical mean power-law approac ...

Atomic force microscopy (AFM) is capable of nanoscale imaging but has so far only been used on cell surfaces when applied to a living cell. Here, we describe a step-by-step protocol for nanoendoscopy-AFM, which enables the imaging of nanoscale structures i ...

Telomeres are nucleoprotein structures at the ends of linear chromosomes, being essential for the maintenance of genomic integrity. Telomeres have a unique structure which distinguishes chromosome termini from DNA damage sites. Shelterin complexes are the ...

The accurate identification of the three-dimensional quantitative shape of a cell nucleus is now possible without fluorescent staining by applying computational segmentation to refractive index tomograms recorded in the flow cytometry mode. ...

Semiconducting single-walled carbon nanotubes (SWCNTs) are among the few photostable optical emitters that are ideal for sensing, imaging, drug delivery, and monitoring of protein activity. These applications often require strategies for immobilizing prote ...

Novel supramolecular gelators based on quinoline-indolin-2-one structure were synthesized. These gelators formed stable organogels in a mixture of DMSO/H2O (1:1). The scanning electron microscopy (SEM), IR and NMR spectroscopies, and rheological measuremen ...

The effect of increasing sulfate contents on the hydration of white cement was studied with a multi-technique approach. Quantitative X-Ray Diffraction (XRD) revealed that adding more gypsum in the cement resulted in more ettringite formation. Combining iso ...

This study aims to investigate the change with temperature of the water content of the C-(A)-S-H of plain cement (PC) and Limestone Calcined Clay Cement (LC3). The water content was determined by various methods: thermogravimetric analysis (TGA), H-1-nucle ...

The abundance and speciation of primary biological aerosol particles (PBAP) is important for understanding their impacts on human health, cloud formation, and ecosystems. Towards this, we have developed a protocol for quantifying PBAP collected from large ...

Cell trackers are fluorescent chemical tools that facilitate imaging and tracking cells within live organisms. Despite their versatility, these dyes lack specificity, tend to leak outside of the cell, and stain neighboring cells. Here, we report a dual-act ...

New dendritic silica/titania mesoporous nanoparticles (DSTNs) loaded with curcumin (CUR) were synthesized and coated with polyethylenimine-folic acid groups (PEI-FA) for an ultrasound (US)-triggered drug release and combined chemo-sonodynamic therapy. The ...

The most basic function of synthetic microenvironments for tissue engineering is to act as a physical substrate for cell attachment, migration, and proliferation, similar to the natural cell environment. Functionalization of supramolecular materials with g ...

At low pH, underwater adherence between poly(N,N-dimethylacrylamide) hydrogels and poly(acrylic acid) brushes is due to the formation of multiple hydrogen bonds. The effect of the key parameters controlling the formation of these interactions (contact time ...

In this work, the antimicrobial effects of the photo-Fenton process on yeast cells were tested (Saccharomyces cerevisiae), an essential eukaryotic unicellular model of living cells. Near-neutral pH was used in all studies, while iron sulfate and iron citra ...

Background: Photodynamic therapy (PDT) has been shown to induce ablation and functional occlusion of tumor-associated lymphatic vessels. However, direct effects of PDT on lymphatic endothelial cells (LECs) have not been studied so far. The aim of this stud ...

The thesis describes the utilization of dative boron-nitrogen bond is used for the construction of new materials. In chapter 2.1, the synthesis of new organogels based on bisimidazolyl ligands and diboronate esters is described. Gels with a critical gel co ...

Fluorescent dyes are widely used for staining and visualization of DNA in optical microscopy based methods. Even though for some dyes the mechanism of binding is known, how this binding affects DNA remains poorly understood. Here we present a novel experim ...

Cell-permeable DNA stains are popular markers in live-cell imaging. Currently used DNA stains for live-cell imaging are either toxic, require illumination with blue light or are not compatible with super-resolution microscopy, thereby limiting their utilit ...

Proteomics has been one of the main projects challenging biological and analytical chemists for many years. The separation, identification and quantification of all the proteins expressed within biological systems remain the main objectives of proteomics. ...

Scanning Electrochemical Microscopy as a Readout Tool for Protein Electrophoresis

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Pore size estimation in axon-mimicking microfibers with diffusion-relaxation MRI

Protocol for live imaging of intracellular nanoscale structures using atomic force microscopy with nanoneedle probes

Telomere protection against oxidative stress

Stain-free identification of cell nuclei using tomographic phase microscopy in flow cytometry

Site-Specific Protein Conjugation onto Fluorescent Single-Walled Carbon Nanotubes

A smart low molecular weight gelator for the triple detection of copper (II), mercury (II), and cyanide ions in water resources

Effect of sulfate on C-S-H at early age

Effect of temperature on the water content of C-A-S-H in plain Portland and blended cements

Using flow cytometry and light-induced fluorescence to characterize the variability and characteristics of bioaerosols in springtime in Metro Atlanta, Georgia

Dual-Activatable Cell Tracker for Controlled and Prolonged Single-Cell Labeling

Design and Synthesis of Gatekeeper Coated Dendritic Silica/Titania Mesoporous Nanoparticles with Sustained and Controlled Drug Release Properties for Targeted Synergetic Chemo-Sonodynamic Therapy

Quantifying Guest-Host Dynamics in Supramolecular Assemblies to Analyze Their Robustness

Equilibrium and Out-of-Equilibrium Adherence of Hydrogels against Polymer Brushes

Evaluation of Auramine O staining and conventional PCR for leprosy diagnosis: A comparative cross-sectional study from Ethiopia

Castles fall from inside: Evidence for dominant internal photo-catalytic mechanisms during treatment of Saccharomyces cerevisiae by photo-Fenton at near-neutral pH

Investigation of cell death mechanisms in human lymphatic endothelial cells undergoing photodynamic therapy

Crystalline and Soft Materials Based on Dative Boron-Nitrogen Bonds

Influence of DNA Binding Dyes on Bare DNA Structure Studied with Atomic Force Microscopy

SiR–Hoechst is a far-red DNA stain for live-cell nanoscopy

New analytical tools combining gel electrophoresis and mass spectrometry