The Use of Valproic Acid in Transient Gene Expression with HEK-293E Cells

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Peroxisomes are eukaryotic organelles that are essential for multiple metabolic pathways, including fatty acid oxidation, degradation of amino acids, and biosynthesis of ether lipids. Consequently, peroxisome dysfunction leads to pediatric-onset neurodegen ...

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Cell fate progression of pluripotent progenitors is strictly regulated, resulting in high human cell diversity. Epigenetic modifications also orchestrate cell fate restriction. Unveiling the epigenetic mechanisms underlying human cell diversity has been di ...

Curcuminoids and their complexes continue to attract attention in medicinal chemistry, but little attention has been given to their metabolic derivatives. Here, the first examples of (arene)Ru(II) complexes with curcuminoid metabolites, tetrahydrocurcumin ...

Two fundamental properties of embryonic stem cells (ESCs) are their ability to self-renew and differentiate into all somatic cell types. Maintenance of their identity faces major challenges when transitioning through mitosis, as most DNA-binding proteins a ...

Co-expression of two or more genes at the single-cell level is usually associated with functional co-regulation. While mRNA co-expression-measured as the correlation in mRNA levels-can be influenced by both transcriptional and post-transcriptional events, ...

Use of autologous cells isolated from elderly patients with multiple comorbidities may account for the modest efficacy of cell therapy in patients with chronic limb threatening ischemia (CLTI). We aimed to determine whether proarteriogenic monocyte/macroph ...

Protein-based therapeutics, such as monoclonal antibodies and cytokines, are important therapies for various pathophysiological conditions such as oncology, autoimmune disorders, and viral infections. However, the wide application of such protein therapeut ...

Scaffold-based protein libraries are designed to be both diverse and rich in functional/folded proteins. However, introducing an extended diversity while preserving stability of the initial scaffold remains a challenge. Here we developed an original approa ...

De novo protein design enhances our understanding of the principles that govern protein folding and interactions, and has the potential to revolutionize biotechnology through the engineering of novel protein functionalities. Despite recent progress in comp ...

Adult autologous human epidermal stem cells can be extensively expanded ex vivo for cell and gene therapy. Identifying the mechanisms involved in stem cell maintenance and defining culture conditions to maintain stemness is critical, because an inadequate ...

SARS-CoV-2 infection requires Spike protein-mediated fusion between the viral and cellular membranes. The fusogenic activity of Spike depends on its post-translational lipid modification by host S-acyltransferases, predominantly ZDHHC20. Previous observati ...

The Use of Valproic Acid in Transient Gene Expression with HEK-293E Cells

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Fluorescent fatty acid conjugates for live cell imaging of peroxisomes

Inheritance of H3K9 methylation regulates genome architecture in Drosophila early embryos

Single-cell epigenomic reconstruction of developmental trajectories from pluripotency in human neural organoid systems

Ru(II)-Arene Complexes of Curcumin and Bisdesmethoxycurcumin Metabolites

Deciphering the molecular events leading to the global restoration of the chromatin landscape during mitotic exit

Exploring the role of transcriptional and post-transcriptional processes in mRNA co-expression

HTATIP2 regulates arteriogenic activity in monocytes from patients with limb ischemia

Rational Design of Chemically Controlled Antibodies and Protein Therapeutics

Design of an artificial phage-display library based on a new scaffold improved for average stability of the randomized proteins

De novo protein design by inversion of the AlphaFold structure prediction network

Low temperature and mTOR inhibition favor stem cell maintenance in human keratinocyte cultures

SARS-CoV-2 hijacks a cell damage response, which induces transcription of a more efficient Spike S-acyltransferase