Publications associées à Widefield High Frame Rate Single-Photon SPAD Imagers for SPIM-FCS

Subsurface fluorescence time-of-flight imaging using a large-format single-photon avalanche diode sensor for tumor depth assessment

Claudio Bruschini, Edoardo Charbon, Arin Can Ülkü, Yichen Feng

Significance: Fluorescence guidance is used clinically by surgeons to visualize anatomical and/or physiological phenomena in the surgical field that are difficult or impossible to detect by the naked eye. Such phenomena include tissue perfusion or molecula ...

SPIE Society of Photo-optical Instrumentation Engineers2024

Pixel super-resolution with spatially entangled photons

Edoardo Charbon, Jiuxuan Zhao

Pixelation is common in quantum imaging systems and limit the image spatial resolution. Here, the authors introduce a pixel super-resolution approach based on measuring the full spatially-resolved joint probability distribution of spatially-entangled photo ...

Nature Portfolio2022

Medipix4, a high granularity four sides buttable pixel readout chip for high resolution spectroscopic X-ray imaging at rates compatible with medical CT scans

Viros Sriskaran

Medipix4 is the latest member in the Medipix/Timepix family of pixel detector chips aimed at high rate spectroscopic X-ray imaging using high-Z materials. The chip address the limitations of conventional hybrid pixel detectors for X-ray imaging. Its predec ...

EPFL2022

Large-Format Time-Gated SPAD Cameras for Real-Time Phasor-Based FLIM

Arin Can Ülkü

Fluorescence lifetime imaging microscopy (FLIM) is an imaging modality often used to monitor biochemical properties of a cell or a tissue. In addition to conventional fluorescence microscopy features, such as selective labeling and non-invasiveness, FLIM e ...

EPFL2021

Towards Quantum 3D Imaging Devices

Claudio Bruschini, Edoardo Charbon, Samuel Burri, Arin Can Ülkü, Paul Mos, Michael Alan Wayne

We review the advancement of the research toward the design and implementation of quantum plenoptic cameras, radically novel 3D imaging devices that exploit both momentum–position entanglement and photon–number correlations to provide the typical refocusin ...

2021

Inkjet-printed polymer composites for the detection of volatile organic compounds

Mohammadmahdi Kiaee

Sensors capable of detecting and classifying volatile organic compounds (VOC) have been gaining more attention by the advent of internet-of-things (IoT) enabled devices and integration of various sensing elements into hand-held and portable devices. The re ...

EPFL2021

Megapixel SPAD cameras for time-resolved applications

Kazuhiro Morimoto

Progress in optics technology has enriched our daily lives in many ways. Various types of single-photon detector technologies have emerged in the last century. Among these, single-photon avalanche diode (SPAD) technology outperforms other detectors for its ...

EPFL2020

Single-molecule localization microscopy

Suliana Manley, Juliette Griffie

Single-molecule localization microscopy (SMLM) describes a family of powerful imaging techniques that dramatically improve spatial resolution over standard, diffraction-limited microscopy techniques and can image biological structures at the molecular scal ...

SPRINGERNATURE2021

Simplified Instrument Calibration forWide-FieldFluorescence Resonance Energy Transfer (FRET) Measured by the Sensitized Emission Method

Forster (or fluorescence) resonance energy transfer (FRET) is a quantifiable energy transfer in which a donor fluorophore nonradiatively transfers its excitation energy to an acceptor fluorophore. A change in FRET efficiency indicates a change of proximity ...

2021

A new kind of atlas of zeolite building blocks

Michele Ceriotti, Benjamin Aaron Helfrecht, Rocio Semino Barbaresi, Giovanni Pireddu

We have analyzed structural motifs in the Deem database of hypothetical zeolites to investigate whether the structural diversity found in this database can be well-represented by classical descriptors, such as distances, angles, and ring sizes, or whether ...

2019

A 512 x 512 SPAD Image Sensor With Integrated Gating for Widefield FLIM

Claudio Bruschini, Edoardo Charbon, Ivan Michel Antolovic, Arin Can Ülkü

In this paper, we report on SwissSPAD2, an image sensor with 512 x 512 photon-counting pixels, each comprising a single-photon avalanche diode (SPAD), a 1-b memory, and a gating mechanism capable of turning the SPAD ON and OFF, with a skew of 250 and 344 p ...

IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC2019

Waveguide platform and methods for super-resolution fluorescence microscopy of sub-cellular structures

Anna Archetti

Super-resolution fluorescence microscopy is widespread, owing to its demonstrated ability to resolve dynamical processes within cells and to identify the structure and position of specific proteins in the interior of protein complexes. Nowadays, subcellula ...

EPFL2019

A time-gated large-array SPAD camera for picosecond resolution real-time FLIM

Claudio Bruschini, Edoardo Charbon, Arin Can Ülkü

Single-photon avalanche diode (SPAD) arrays have recently emerged as promising detectors for many wide-field fluorescence lifetime imaging microscopy (FLIM) applications, thanks to their picosecond range temporal resolution, single-photon sensitivity and f ...

2018

Applications of a reconfigurable SPAD line imager

Claudio Bruschini, Edoardo Charbon, Samuel Burri

Single-photon avalanche diodes (SPADs) fabricated in standard CMOS processes enable cost-effective volume production of sensors capable of time-stamping individual photons with high accuracy. This capability makes the sensors ideally suited for application ...

2018

Nanoscale Lithography and Thermometry with Thermal Scanning Probes

Samuel Tobias Howell, Samuel Zimmermann

Thermal scanning probe lithography (t-SPL) is an advanced lithography technique in which a heated atomic force microscope tip locally modifies a sample material. Due to the nanometer-sized tip apex diameter, t-SPL enables patterning of sub-10 nm structures ...

EPFL2018

Imaging free and bound NADH towards cancer tissue detection using FLIM system based on SPAD array

Claudio Bruschini, Edoardo Charbon, Samuel Burri, Ivan Michel Antolovic

Summary form only given. Single photon detectors allow us to work with the weakest signals such as auto-fluorescent biological sources. In combination with time gated operation mode, an array of detectors can be used as Fluorescence Lifetime Imaging system ...

2017

SPAD imagers for super resolution localization microscopy enable analysis of fast fluorophore blinking

Claudio Bruschini, Edoardo Charbon, Samuel Burri, Ivan Michel Antolovic

sCMOS imagers are currently utilized (replacing EMCCD imagers) to increase the acquisition speed in super resolution localization microscopy. Single-photon avalanche diode (SPAD) imagers feature frame rates per bit depth comparable to or higher than sCMOS ...

Nature Research2017

Nanopatterning of a Stimuli-Responsive Fluorescent Supramolecular Polymer by Thermal Scanning Probe Lithography

Jürgen Brugger, Samuel Zimmermann

The miniaturization of nanometer-sized multicolor fluorescent features is of continuous significance for counterfeit security features, data storage, and sensors. Recent advances in engineering of stimuli-responsive supramolecular polymeric materials that ...

2017

Fluorescence lifetime imaging using a single photon avalanche diode array sensor

Claudio Bruschini, Edoardo Charbon, Samuel Burri, Ivan Michel Antolovic

Single photon detectors allows us work with the weakest fluorescence signals. Single photon arrays, combined with ps-controlled gating allow us to create image maps of fluorescence lifetimes, which can be used for in-vivo discrimination of tissue activity. ...

2017

Coherent Imaging of Cellular Dynamics

Miguel Sison

Mitochondrial dynamics refers to the processes of fusion, fission, and transport that aid mitochondria in accomplishing their many roles; including ATP production, oxygen sensing, and homeostasis. Due to their involvement in numerous essential cellular act ...

EPFL2017