Optimised biomolecular extraction for metagenomic analysis of microbial biofilms from high-mountain streams

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The COVID-19 pandemic has highlighted the necessity to develop fast, highly sensitive and selective virus detection methods. Surface-based DNA-biosensors are interesting candidates for this purpose. Functionalization of solid substrates with DNA must be pr ...

DNA-binding proteins physically interact with the DNA and directly affect genomic functions. The eukaryotic genome is compacted into chromatin, limiting the DNA access to nuclear factors. In this Ph.D. thesis, I explored the dynamic mechanisms, that allow ...

The present invention relates generally to the field of nucleic acid sequencing and provides oligonucleotide molecules and barcodes contained therein. These oligonucleotide molecules and barcodes molecules are useful in sequencing to identify and resolve e ...

Reduction-oxidation (redox) reactions underlie essentially all biogeochemical cycles. Like most soil properties and processes, redox is spatiotemporally heterogeneous. However, unlike other soil features, redox heterogeneity has yet to be incorporated into ...

Streams draining proglacial floodplains harbor benthic biofilms comprised of diverse microbial communities. These high-mountain ecosystems are rapidly changing with climate warming, and it is therefore critical to better understand the mechanisms underlyin ...

The present invention provides a method for preparing a sequencing library from a ribonucleic acid (RNA) sample. ...

DNA fragmentation is an essential process in developing genetic sequencing strategies, genetic research, as well as for the diagnosis of diseases with a genetic signature like cancer. Efficient on-chip DNA fragmentation protocols would be beneficial to pro ...

Polymerase chain reaction (PCR) has been the most significant driver in the field of nucleic acid testing (NAT) since its invention. Popularized as an abbreviation by the Covid-19 pandemic, PCR-based methods are the gold standard in the field of diagnostic ...

Benchmarking of scATAC-seq protocols demonstrates the effects of data quality on downstream analyses. ...

Throughout the global COVID-19 pandemic, SARS-CoV-2 genetic variants of concern (VOCs) have repeatedly and independently arisen. VOCs are characterized by increased transmissibility, increased virulence, or reduced neutralization by antibodies obtained fro ...

Next-generation sequencing (NGS) is an essential technology for DNA identification in genomic research. DNA fragmentation is a critical step for NGS and doing this on-chip is of great interest for future integrated genomic solutions. Here we demonstrate fa ...

The evolution of the 3D morphology is at the center of many relevant biological processes ranging from cellular differentiation to cancer invasion and metastasis. Microscopy techniques, such as electron microscopy (EM), super-resolution (SR) optical micros ...

In ice-covered lakes, near-bottom oxygen concentration decreases for most of the wintertime, sometimes down to the point that bottom waters become hypoxic. Studies insofar have reached divergent conclusions on whether climate change limits or reinforces th ...

Engineering and computational advances have opened many new avenues in cancer research, particularly when being exploited in interdisciplinary approaches. For example, the combination of microfluidics, novel sequencing technologies, and computational analy ...

The dynamic of fine sediment in rivers is closely related to the interactions between fine particles, the riverbed and the flow conditions. The accumulation of fine sediment in the riverbed reduces vertical water exchanges and can have detrimental effects ...

Shearing DNA to a certain size is the first step in many medical and biological applications, especially in next-generation gene sequencing technology. In this article, we introduced a highly efficient ultrasonic DNA fragmentation method enhanced by needle ...

Riverbeds represent the habitat of numerous aquatic species. Exchanges between the groundwater, the hyporheic zone and the surface flow are also essential for river ecosystems. Fine sediment transported by rivers deposits inside or on top of the bed and mo ...

Optimised biomolecular extraction for metagenomic analysis of microbial biofilms from high-mountain streams

Chattez avec Graph Search

Achieving high hybridization density at DNA biosensor surfaces using branched spacer and click chemistry

Development of multiplexed single-molecule imaging techniques to study chromatin invasion dynamics of chromatin effector proteins

Optimised set of oligonucleotides for bulk rna barcoding and sequencing

Consider the Anoxic Microsite: Acknowledging and Appreciating Spatiotemporal Redox Heterogeneity in Soils and Sediments

Homogeneous Environmental Selection Structures the Bacterial Communities of Benthic Biofilms in Proglacial Floodplain Streams

Construction of sequencing libraries from a ribonucleic acid (rna) using tailing and ligation of cdna (tlc)

Bubble-enhanced ultrasonic microfluidic chip for rapid DNA fragmentation

Electronic readout of DNA amplification in nanoliter chambers. Strategies towards highly parallel semiconductor-based nucleic acid amplification testing

Systematic benchmarking of single-cell ATAC-sequencing protocols

Inferring Transmission Fitness Advantage of SARS-CoV-2 Variants of Concern in Wastewater Using Digital PCR

Polydimethylsiloxane microstructure-induced acoustic streaming for enhanced ultrasonic DNA fragmentation on a microfluidic chip

Time-Resolved Scanning Ion Conductance Microscopy and Single-Molecule Spectroscopy

Near-bed stratification controls bottom hypoxia in ice-covered alpine lakes

Technological and computational advances driving high-throughput oncology

Influence of the shear stress on fine sediment exchanges with the substrate using UVP measurements

The enhancement of DNA fragmentation in a bench top ultrasonic water bath with needle-induced air bubbles: Simulation and experimental investigation

Clogging of riverbed substrates by fine sediment