Fluorescent magnetic bead and cell differentiation/counting using a CMOS SPAD matrix

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We introduce a droplet-digital microfluidic recovery system to collect single cells from pooled culture and transfer these droplets into a well-plate. The platform is shown for the recovery of single beads and rare antibody secreting cells. ...

In the age of smart systems, artificial intelligence, and personalized devices, the need for effective methods to facilitate multifunctionality integration has become a necessity. One of the fields that boosts the efforts of multifunctionality integration ...

Fluorescent probes are an indispensable tool in the realm of bioimaging technologies, providing valuable insights into the assessment of biomaterial integrity and structural properties. However, incorporating fluorophores into scaffolds made from melt elec ...

The invention relates to a fluorometer system for analyzing fluid samples comprising a plurality of particles capable of being labeled with at least two fluorescent labels, in particular two distinct fluorophores. The invention also relates to a fluorescen ...

Significance: Fluorescence guidance is used clinically by surgeons to visualize anatomical and/or physiological phenomena in the surgical field that are difficult or impossible to detect by the naked eye. Such phenomena include tissue perfusion or molecula ...

Redox homeostasis is a key factor in maintaining cellular function and health. The main determinant of the intracellular redox potential is ubiquitous glutathione (GSH) together with its oxidized dimer (GSSG). Importantly, both redox equilibrium and GSH ho ...

The invention provides microfluidic screening systems for the identification of compounds or compositions influencing cellular transcriptomes. The invention is predicated upon taking into account differences in newly synthesized mRNA in contrast to so call ...

As the fundamental machinery orchestrating cellular functions, proteins influence the state of every cell profoundly. As cells exhibit significant variations from one to another, analyzing the proteome on a single-cell level is imperative to unravel their ...

Recordings of the physiological history of cells provide insights into biological processes, yet obtaining such recordings is a challenge. To address this, we introduce a method to record transient cellular events for later analysis. We designed proteins t ...

Microfluidic models are proving to be powerful systems to study fundamental processes in porous media, due to their ability to replicate topologically complex environments while allowing detailed, quantitative observations at the pore scale. Yet, while por ...

In droplet microfluidic devices with suction-based flow control, the microchannel geometry and suction pressure at the outlet govern the dynamic properties of the two phases that influence the droplet generation. Therefore, it is critical to understand the ...

Networks of tryptophan (Trp)& horbar;an aromatic amino acid with strong fluorescence response & horbar;are ubiquitous in biological systems, forming diverse architectures in transmembrane proteins, cytoskeletal filaments, subneuronal elements, photorecepto ...

Microorganisms, encompassing both uni- and multicellular entities, exhibit remarkable diversity as omnipresent life forms in nature. They play a pivotal role by supplying essential components for sustaining biological processes across diverse ecosystems, i ...

Mycobacterium Tuberculosis is a highly effective pathogen infecting nearly a third of the world's population. An M. tuberculosis infection starts when droplets containing bacteria enter an individual's lungs. The first host cells to contact the bacteria ar ...

Communication between the intestine and other organs such as the lungs, brain or bones is mediated by several metabolites, like short-chain fatty acids or bile acids, that relay information about nutritional and microbiota status. Bile acids are endogenous ...

Cells are the smallest operational units of living systems. Through synthesis of various biomolecules and exchange of signals with the environment, cells tightly regulate their composition to realize a specific functional state. The transformation of a cel ...

Traditional cell cultures have long been fundamental to biological research, offering an alternative to animal models burdened by ethical constraints and procedural intricacies, often lacking relevance to human physiology and disease. Moreover, their inabi ...

Surface-based biosensors have proven to be of particular interest in the monitoring of human pathogens by means of their distinct nucleic acid sequences. Genosensors rely on targeted gene/DNA probe hybridization at the surface of a physical transducer and ...

In this thesis, we explored on-chip high-resolution imaging of the fate of intestinal bacteria and bacterial products in Caenorhabditis elegans (C. elegans). In the first part, we carried out high-resolution z-stack fluorescent imaging of Red Fluorescent P ...

These datasets contain the total particle number concentrations and normalized size distributions (dN/dlogDp) of excited, fluorescent, and hyper-fluorescent particles of sizes 0.5 to 20 μm (optical diameter). The normalized size distribution datasets are s ...

Fluorescent magnetic bead and cell differentiation/counting using a CMOS SPAD matrix

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Recovery of phenotypically sorted cells using droplet-digital microfluidics

Additive Fabrication of Functional Microfluidic Devices

[n]Cycloparaphenylenes as Compatible Fluorophores for Melt Electrowriting

Fluorometer system, fluorescene analysis method, and use thereof

Subsurface fluorescence time-of-flight imaging using a large-format single-photon avalanche diode sensor for tumor depth assessment

HaloTag-Based Tools for Live-Cell Imaging and Elucidation of Organellar Redox Biology

Improved microfluidic transcriptome based screening methods

Development of tools and methods for protein identification: From single molecules to in vivo applications

Recording physiological history of cells with chemical labeling

Multiscale Porosity Microfluidics to Study Bacterial Transport in Heterogeneous Chemical Landscapes

Modified capillary number to standardize droplet generation in suction-driven microfluidics

Ultraviolet Superradiance from Mega-Networks of Tryptophan in Biological Architectures

Microfluidic systems for infectious disease diagnostics

Single-cell analysis of the interactions between Mycobacterium tuberculosis and macrophages

Characterization of the gut-bone marrow axis through bile acid signaling

Deciphering the nature of cell state transitions in single cells using quantitative modeling of temporal dynamics

Accessible and highly observable gastrointestinal organoid model systems for studying host-pathogen interactions

“Grafting-from” and “Grafting-to” Poly(N-isopropyl acrylamide) Functionalization of Glass for DNA Biosensors with Improved Properties

Microfluidic devices for automated analysis of ingested bacteria in the Caenorhabditis elegans intestine

Wideband integrated bioaerosol sensor (WIBS) excited, fluorescent, and hyper-fluorescent particle number concentrations and normalized size distributions (dN/dlogDp) measured in the Swiss container during MOSAiC 2019/2020