Characterization of Efficiency and Mechanisms of Cold Atmospheric Pressure Plasma Decontamination of Seeds for Sprout Production

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Bacteria are ubiquitous single cellular organisms. Compared to eukaryotic cells, bacteria have two unique characteristics: the membrane-less nucleoid and the cell wall built of peptidoglycan (PG). In most bacteria, a single circular chromosome is compacted ...

The World Health Organization highlights the urgent need to address the global threat posed by antibiotic-resistant bacteria. Efficient and rapid detection of bacterial response to antibiotics and their virulence state is crucial for the effective treatmen ...

Fluorescence confocal laser-scanning microscopy (LSM) is one of the most popular tools for life science research. This popularity is expected to grow thanks to single-photon array detectors tailored for LSM. These detectors offer unique single-photon spati ...

Ribosomes translate the genetic code into proteins. Recent technical advances have facilitated in situ structural analyses of ribosome functional states inside eukaryotic cells and the minimal bacterium Mycoplasma. However, such analyses of Gram-negative b ...

Atmospheric pressure low power plasma jets operating in noble gases are a widespread tool in plasma medicine studies. We present experimental results obtained in one such device, which combine physical, chemical and biological measurements to assess the ef ...

Biomolecular condensates play important roles in a wide array of fundamental biological processes, such as cellular compartmentalization, cellular regulation, and other biochemical reactions. Since their discovery and first observations, an extensive and e ...

Significance: Fluorescence guidance is used clinically by surgeons to visualize anatomical and/or physiological phenomena in the surgical field that are difficult or impossible to detect by the naked eye. Such phenomena include tissue perfusion or molecula ...

After decades of technological advancements, high-speed atomic force microscopy (HS-AFM) has emerged as a powerful technique for visualizing dynamic processes. At the nanoscale, the AFM provides valuable insights into the sample by sensing minute interacti ...

Aerosol transmission remains a major challenge for control of respiratory viruses, particularly those causing recurrent epidemics, like influenza A virus (IAV). These viruses are rarely expelled alone, but instead are embedded in a consortium of microorgan ...

Antibiotic resistance is nowadays a major public health issue. Rapid antimicrobial susceptibility tests (AST) are one of the options to fight this deadly threat. Performing AST with single-cell sensitivity that is rapid, cheap, and widely accessible, is ch ...

We study the presence within the worm Caenorhabditis elegans (C. elegans) of a fluorescent strain of the worm's bacterial food (Escherichia coli (E. coli) OP50) during early adulthood. Use of a microfluidic chip based on a thin glass coverslip substrate al ...

Bacteria often colonize their environment in the form of surface attached multicellular communities called biofilms. Biofilms grow from surface-attached cells that undergo division while self-embedding in a viscoelastic matrix. Biofilms grow at the surface ...

The evolution of the 3D morphology is at the center of many relevant biological processes ranging from cellular differentiation to cancer invasion and metastasis. Microscopy techniques, such as electron microscopy (EM), super-resolution (SR) optical micros ...

In this thesis, we explored on-chip high-resolution imaging of the fate of intestinal bacteria and bacterial products in Caenorhabditis elegans (C. elegans). In the first part, we carried out high-resolution z-stack fluorescent imaging of Red Fluorescent P ...

The abusive use of antimicrobial drugs during the last eighty years has favoured the natural selection of resistant pathogens able to neutralise drugs efficiently. Nowadays, antimicrobial resistance contributes to millions of deaths yearly, threatens the e ...

We discuss the properties of signal strength and integrated intensity in two-photon excitation confocal microscopy and image scanning microscopy. The resolution, optical sectioning and background rejection are all improved over nonconfocal two-photon micro ...

The properties of signal strength and integrated intensity in a scanned imaging system are reviewed. These prop-erties are especially applied to confocal imaging systems, including image scanning microscopy. The integrated intensity, equal to the image of ...

Bacteria often engage in social interactions with neighbouring bacteria. Ecosystems which are subjected to social interactions have been widely studied in well mixed settings such as test tubes, allowing us to identify the cellular components contributing ...

The pathogenic bacterium Streptococcus pneumoniae (S. pneumoniae) can invade the cerebrospinal fluid (CSF) and cause meningitis with devastating consequences. Whether and how sensory cells in the central nervous system (CNS) become activated during bacteri ...

Optical microscopy is an essential tool for biologists, who are often faced with the need to overcome the spatial and temporal resolution limitations of their devices to capture finer details. As upgrading imaging hardware is expensive, computational metho ...

Characterization of Efficiency and Mechanisms of Cold Atmospheric Pressure Plasma Decontamination of Seeds for Sprout Production

Chattez avec Graph Search

Resolving bacterial cell biology: from replisome dynamics to cell wall synthesis

Machine learning method for the classification of the state of living organisms' oscillations

Compact and effective photon-resolved image scanning microscope

Bactericidal effect of tetracycline in E. coli strain ED1a may be associated with ribosome dysfunction

Combined Characterization of a Plasma Jet: FTIR, Chemical Surveys and Antibacterial Effect

Label-Free Techniques for Probing Biomolecular Condensates

Subsurface fluorescence time-of-flight imaging using a large-format single-photon avalanche diode sensor for tumor depth assessment

Advances in High-Speed, Multiparametric Atomic Force Microscopy

Stability of influenza A virus in droplets and aerosols is heightened by the presence of commensal respiratory bacteria

Simple optical nanomotion method for single-bacterium viability and antibiotic response testing

High-resolution imaging and analysis of the intestinal bacterial load of Caenorhabditis elegans during early adulthood

Biofilm morphogenesis on soft hydrogels

Time-Resolved Scanning Ion Conductance Microscopy and Single-Molecule Spectroscopy

Microfluidic devices for automated analysis of ingested bacteria in the Caenorhabditis elegans intestine

Antimicrobial susceptibility testing with integrated photonic crystal optical tweezers

Background Rejection in Two-Photon Fluorescence Image Scanning Microscopy

Signal strength and integrated intensity in confocal and image scanning microscopy

The Effects of Spatial Organization and Fluid Flow on the Social Interactions in Microbial Communities

CSF-contacting neurons respond to Streptococcus pneumoniae and promote host survival during central nervous system infection

Practical computational imaging by use of spatiotemporal light modulation: from simulations to applications in biological microscopy