Small-Molecule Fluorescent Probes for Live-Cell Super-Resolution Microscopy

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The visualization and manipulation of proteins in live cells are critical for studying complex biological processes. Self-labeling proteins do so by enabling the specific and covalent attachment of synthetic probes, offering unprecedented flexibility in th ...

Cyclic peptides are attractive for drug discovery due to their excellent binding properties and the potential to cross cell membranes. However, by far not all cyclic peptides are cell permeable, and measuring or predicting their membrane permeability is no ...

Whole-cell biophotovoltaic (BPV) devices leverage photoautotrophic microorganisms like cyanobacteria to convert sunlight and water into bioelectricity. They are capable of growing on sequestered carbon dioxide (CO2), eliminating the need to add exogenous o ...

Volumetric 3D printing enables the rapid fabrication of centimetre-scale objects, with the fastest techniques requiring only a few seconds. Having emerged during the past 7 years, this new family of technologies is posed to revolutionize additive manufactu ...

Pacinian corpuscles are among the most sensitive mechanoreceptors found in vertebrates, and they are tuned to vibrations in the highest perceptible frequency range (100 to 2000 Hz). One of their anatomical hallmarks is the onion-like cell layers surroundin ...

Advancing quantum technologies depends on the precise control of individual quantum systems, the so-called qubits, and the exploitation of their quantum properties. Nowadays, expanding the number of qubits to be entangled is at the core of the developments ...

In this thesis, we explored on-chip high-resolution imaging of the fate of intestinal bacteria and bacterial products in Caenorhabditis elegans (C. elegans). In the first part, we carried out high-resolution z-stack fluorescent imaging of Red Fluorescent P ...

Polo-like kinase (Plk4) is a serine/threonine-proteinkinase thatis essential for biogenesis of the centriole organelle and is enrichedat centrioles. Herein, we introduce Cen-TCO, a chemical probe basedon the Plk4 inhibitor centrinone, to image Plk4 and cen ...

Significance: Fluorescence guidance is used clinically by surgeons to visualize anatomical and/or physiological phenomena in the surgical field that are difficult or impossible to detect by the naked eye. Such phenomena include tissue perfusion or molecula ...

Optical microscopy is an essential tool for biologists, who are often faced with the need to overcome the spatial and temporal resolution limitations of their devices to capture finer details. As upgrading imaging hardware is expensive, computational metho ...

Fluorescence confocal laser-scanning microscopy (LSM) is one of the most popular tools for life science research. This popularity is expected to grow thanks to single-photon array detectors tailored for LSM. These detectors offer unique single-photon spati ...

We discuss the properties of signal strength and integrated intensity in two-photon excitation confocal microscopy and image scanning microscopy. The resolution, optical sectioning and background rejection are all improved over nonconfocal two-photon micro ...

Histone deacylases are erasers of Nε-acyl-lysine post-translational modifications and have been targeted for decades for the treatment of cancer, neurodegeneration and other disorders. Due to their relatively promiscuous activity on peptide substrates in v ...

Fundamental properties of light unavoidably impose features on images collected using fluorescence microscopes. Accounting for these features is often critical in quantitatively interpreting microscopy images, especially those gathering information at scal ...

We propose an image-based elastography method to measure the heterogeneous stiffness inside a cell and its nucleus. It uses a widely accessible setup consisting of plate compression imaged with fluorescence microscopy. Our framework recovers a spatial map ...

The proliferation of microscopy methods for live-cell imaging offers many new possibilities for users but can also be challenging to navigate. The prevailing challenge in live-cell fluorescence microscopy is capturing intra-cellular dynamics while preservi ...

State-of-the-art object detection and segmentation methods for microscopy images rely on supervised machine learning, which requires laborious manual annotation of training data. Here we present a self-supervised method based on time arrow prediction pre-t ...

Interferometric scattering (iSCAT) microscopy enables the label-free observation of biomolecules. Consequently, single-particle imaging and tracking with the iSCAT-based method known as mass photometry (MP) is a growing area of study. However, establishing ...

Recent advances in volume electron microscopy (EM) have been driving our thorough understanding of the brain architecture. Volume EM becomes increasingly powerful when cells and their subcellular structures that are imaged in light microscopy are correlate ...

Fluorescence imaging is an invaluable tool to study biological processes and further progress depends on the development of advanced fluorogenic probes that reach intracellular targets and label them with high specificity. Excellent fluorogenic rhodamine d ...

Small-Molecule Fluorescent Probes for Live-Cell Super-Resolution Microscopy

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Exploiting Covalent Chemical Labeling with Self-Labeling Proteins

Bulk measurement of membrane permeability for random cyclic peptides in living cells to guide drug development

Bioanode engineering for living solar cells

The road ahead in materials and technologies for volumetric 3D printing

Lamellar Schwann Cells in the Pacinian Corpuscle Potentiate Vibration Perception

Electron-beam based nanoscale quantum controls

Microfluidic devices for automated analysis of ingested bacteria in the Caenorhabditis elegans intestine

Chemical Probe for Imaging of Polo-like Kinase 4 and Centrioles

Subsurface fluorescence time-of-flight imaging using a large-format single-photon avalanche diode sensor for tumor depth assessment

Practical computational imaging by use of spatiotemporal light modulation: from simulations to applications in biological microscopy

Compact and effective photon-resolved image scanning microscope

Background Rejection in Two-Photon Fluorescence Image Scanning Microscopy

Revealing chromatin-specific functions of histone deacylases

Fluorescence microscopy: A statistics-optics perspective

PROBING INTRACELLULAR ELASTICITY WITH MINIMAL-HESSIAN REGISTRATION

Live-cell imaging powered by computation

Self-supervised Dense Representation Learning for Live-Cell Microscopy with Time Arrow Prediction

Surface passivation and functionalisation for mass photometry

Correlative light and volume electron microscopy to study brain development

A general strategy to develop fluorogenic polymethine dyes for bioimaging