Generation of plasmid DNA for the application of transient transfection in mammalian cells

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Centrosomes act as the main microtubule-organizing centre of animal cells and play critical roles in the cell, such as mitotic spindle organization, cell polarity, and motility. They are composed of two barrel-shaped structures, the centrioles, surrounded ...

Technology is playing an increasing role in our society. Therefore it becomes important to educate the general public, and young generations in particular, about the most common technologies. In this context, robots are excellent education tools, for many ...

This chapter is a contribution in honour of Gerhard H Jirka, who has been fascinated by the amazing variety of small-scale structures that nature surprises us with, particularly in stratified natural waters. Here, we focus on the diffusive regime of double ...

Optical frequency combs allow for the precise measurement of optical frequencies and are used in a growing number of applications. The new class of Kerr-frequency comb sources, based on parametric frequency conversion in optical microresonators, can comple ...

Here we present an electrical lysis throughput of 600 microliters per minute at high cell density (108 yeast cells per ml) with 90% efficiency, thus improving the current common throughput of one microliter per minute. We also demonstrate the extraction of ...

We develop second-harmonic nanoparticles as the contrast agents for cell imaging. Second-harmonic nanoparticles show promise as cell imaging probes due to their non-bleaching, non-blinking, and coherent signal. Nanoparticles of noncentrosymmetric crystal s ...

We report a "plug and play" single photon source, fully integrated with an optical fiber, emitting at 1.3 mu m. Micropillars were patterned on a single layer InAs quantum dot wafer to guarantee a single pillar per fiber core. The single exciton peak filter ...

Due to its surface sensitivity and high spatial resolution, scanning near-field optical microscopy (SNOM) has a significant potential to study the lateral organization of membrane domains and clusters. Compared to other techniques, infrared near-field micr ...

IEEE 802.11 works properly only if the stations respect the MAC protocol. We show in this paper that a greedy user can substantially increase his share of bandwidth, at the expense of the other users, by slightly modifying the driver of his network adapter ...

Recombinant proteins are gaining in importance for therapeutic applications. The proteins are expressed in stable cell lines, within which recombinant DNA has incorporated into the host cells genome. Identification and isolation of extremely high producers ...

Generation of plasmid DNA for the application of transient transfection in mammalian cells

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Purification of centrosomes from mammalian cell lines

A two years informal learning experience using the Thymio robot

Diffusive-type of double diffusion in lakes - a review

Universal formation dynamics and noise of Kerr-frequency combs in microresonators

Very High Throughput Electrical Cell Lysis and Extraction of Intracellular Compounds Using 3D Carbon Electrodes in Lab-on-a-Chip Devices

Characterization of the cytotoxicity and imaging properties of secondharmonic nanoparticles

Oxidation of Catecholboron Enolates with TEMPO

"Plug and Play" single photons at 1.3 mu m approaching gigahertz operation

Infrared scanning near-field optical microscopy investigates order and clusters in model membranes

DOMINO: Detecting MAC layer greedy behavior in IEEE 802.11 hotspots

Establishment of recombinant mammalian cell lines : new approaches