Publications associées à Top-down and middle-down mass spectrometry structural characterization of monoclonal antibodies

Publications associées (21)

Top-Down and Middle-Down Mass Spectrometry of Antibodies

This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional c ...

Elsevier BV2025

Optimizing photon capture: advancements in amorphous silicon-based microchannel plates

Edoardo Charbon, Christophe Ballif, Claudio Bruschini, Nicolas Würsch, Luca Massimiliano Antognini, Emanuele Ripiccini, Jad Benserhir, Samira Alexandra Frey

Microchannel plates are electron multipliers widely used in applications such as particle detection, imaging, or mass spectrometry and are often paired with a photocathode to enable photon detection. Conventional microchannel plates, made of glass fibers, ...

2025

Adding Color to Mass Spectra of Biopolymers: Charge Determination Analysis (CHARDA) Assigns Charge State to Every Ion Peak

Yury Tsybin, Natalia Gasilova, Laure Menin, Anton Kozhinov, Konstantin Nagornov

Traditionally, mass spectrometry (MS) output is the ion abundance plotted versus the ionic mass-to-charge ratio m/z. While employing only commercially available equipment, Charge Determination Analysis (CHARDA) adds a third dimension to MS, estimating for ...

Amer Chemical Soc2024

Gradients of Deposition and In Situ Production Drive Global Glacier Organic Matter Composition

Tom Ian Battin, Hannes Markus Peter, Matteo Tolosano, Vincent Henri De Staercke, Michail Styllas, Martina Andrea Schön

Runoff from rapidly melting mountain glaciers is a dominant source of riverine organic carbon in many high-latitude and high-elevation regions. Glacier dissolved organic carbon is highly bioavailable, and its composition likely reflects internal (e.g., aut ...

2024

Direct observation of glycans bonded to proteins and lipids at the single-molecule level

Klaus Kern, Stephan Rauschenbach

Proteins and lipids decorated with glycans are found throughout biological entities, playing roles in biological functions and dysfunctions. Current analytical strategies for these glycan-decorated biomolecules, termed glycoconjugates, rely on ensemble-ave ...

American Association for the Advancement of Science2023

Bridging Native and Intrinsic Structures of Microhydrated Biomolecules by Cold Ion Spectroscopy

Andrei Zviagin

Solving native structures of such large molecules, like biomolecules, is often challenging, particularly due to the potentially infinite number of non-covalent interactions with water. In this thesis, we report the use of cold ion gas-phase action spectros ...

EPFL2023

Proteomics of human biological fluids for biomarker discoveries: technical advances and recent applications

Loïc Dayon

Introduction Biological fluids are routine samples for diagnostic testing and monitoring. Blood samples are typically measured because of their moderate invasive collection and high information content on health and disease. Several body fluids, such as ce ...

TAYLOR & FRANCIS LTD2022

Characterization of the Time-Domain Isotopic Beat Patterns of Monoclonal Antibodies in Fourier Transform Mass Spectrometry

Natalia Gasilova, Laure Menin

The time-domain transients in the Fourier transform mass spectrometry (FTMS) analysis of monoclonal antibodies (mAbs) are known to exhibit characteristic isotopic beat patterns. These patterns are defined by the isotopic distributions of all gaseous mAb io ...

2022

Combining multi-stage ion mobility spectrometry with cryogenic infrared spectroscopy for glycan analysis

Priyanka Bansal

Structural analysis of biomolecules is essential to understanding biological processes. Among the various classes of biomolecules, glycans have gained significant attention recently due to their ubiquitous roles in biological systems. However, study of gly ...

EPFL2022

A method and system to build an ir fingerprint database for the structural identification of biomolecules

Thomas Rizzo, Ahmed Ben Faleh, Stephan Warnke, Priyanka Bansal, Robert Paul Pellegrinelli

A method, system, and computer-readable medium for identifying and creating a database of isomers and isobars of molecules including the steps of performing isomer or isobar separation on molecules to obtain separate isomeric or isobaric molecules, measuri ...

2022

Analyzing glycans cleaved from a biotherapeutic protein using a combination of ion mobility spectrometry and cryogenic ion spectroscopy

Natalia Yalovenko

The characterization of biomolecules plays a central role in biomedical research. The development of state-of-the-art technologies is needed to make such analyses faster, more sensitive, and more accurate. While mass spectrometry continues to play a centra ...

EPFL2021

Photoisomerization of Linear and Stacked Isomers of a Charged Styryl Dye: A Tandem Ion Mobility Study

Eduardo Carrascosa Casado

The photoisomerization behavior of styryl 9M, a common dye used in material sciences, is investigated using tandem ion mobility spectrometry (IMS) coupled with laser spectroscopy. Styryl 9M has two alkene linkages, potentially allowing for four geometric i ...

AMER CHEMICAL SOC2021

Spatially-distributed cyclotron oscillators approach to FT-ICR MS at the true cyclotron frequency: Computational evaluation of sensitivity

Yury Tsybin, Anton Kozhinov, Konstantin Nagornov

Routine Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) with ion detection at the unreduced (true) cyclotron frequency, instead of the reduced cyclotron frequency, has recently been demonstrated experimentally and supported computat ...

ELSEVIER2021

Proteomics of Human Milk: Definition of a Discovery Workflow for Clinical Research Studies

Loïc Dayon

Milk is a complex biological fluid composed mainly of water, carbohydrates, lipids, proteins, and diverse bioactive factors. Human milk represents a unique tailored source of nutrients that adapts during lactation to the specific needs of the developing in ...

2021

Observation of new excited Bs0 states

Olivier Schneider, Aurelio Bay, Guido Haefeli, Tatsuya Nakada, Frédéric Blanc, Lesya Shchutska, Elena Graverini, Michel De Cian, Vladimir Macko, Federico Leo Redi, Sebastian Schulte, Tara Nanut, Minh Tâm Tran, Violaine Bellée, Guillaume Max Pietrzyk, Pavol Stefko, Pietro Marino, Maria Vieites Diaz, Marie Theres Christin Bachmayer, Lino Ferreira Lopes, Matthieu Philippe Luther Marinangeli, Serhii Cholak, Veronica Sølund Kirsebom, Maria Elena Stramaglia, Surapat Ek-In, Ana Bárbara Rodrigues Cavalcante, Sara Celani, Plamen Hristov Hopchev, Carina Trippl, Sonia Amina Bouchiba, Thi Dung Nguyen, Alison Maria Tully, Mâu Chung Nguyên

A structure is observed in the B +/- K -/+ mass spectrum in a sample of proton-proton collisions at centre-of-mass energies of 7, 8, and 13 TeV, collected with the LHCb detector and corresponding to a total integrated luminosity of 9

SPRINGER2021

CO2 Hydrogenation over Unsupported Fe-Co Nanoalloy Catalysts

Andreas Züttel, Kun Zhao, Robin Tobias Andreas Mutschler, Marco Calizzi

The thermo-catalytic synthesis of hydrocarbons from CO2 and H2 is of great interest for the conversion of CO2 into valuable chemicals and fuels. In this work, we aim to contribute to the fundamental understanding of the effect of alloying on the reaction y ...

2020

Separation and Identification of Glycan Anomers Using Ultrahigh-Resolution Ion Mobility Spectrometry and Cryogenic Ion Spectroscopy

Thomas Rizzo, Ahmed Ben Faleh, Stephan Warnke, Valeriu Scutelnic

The analysis of carbohydrates, or glycans, is challenging for established structure-sensitive gas-phase methods. The multitude of possible stereo-, regio- and structural isomers make them substantially more complex to ana-lyze than DNA or proteins, and no ...

2019

Method for Identification of Threonine Isoforms in Peptides by Ultraviolet Photofragmentation of Cold Ions

Oleg Boyarkine, Vladimir Kopysov, Aleksandr Pereverzev

Identification of isomeric amino acid residues in peptides and proteins is challenging but often highly desired in proteomics. One of the practically important cases that require isomeric assignments is that associated with single-nucleotide polymorphism s ...

AMER CHEMICAL SOC2019

Investigation of deuterium trapping and release in the JET ITER-like wall divertor using TDS and TMAP

Olivier Sauter, Richard Pitts, Ambrogio Fasoli, Stefano Coda, Basil Duval, Henri Weisen, Yves Martin, Yann Camenen, Jan Horacek, Javier García Hernández, Duccio Testa, Marco Wischmeier, Nicola Vianello, Mikhail Maslov, Patrick Blanchard, Alessandro Pau, David Pfefferlé, Davide Galassi, Antonio José Pereira de Figueiredo, Liang Yao, Dalziel Joseph Wilson, Cristian Sommariva, Jonathan Graves, Federico Nespoli, Hamish William Patten, Samuel Lanthaler, Jonathan Marc Philippe Faustin

Selected set of samples from JET ITER-Like Wall (JET-ILW) divertor tiles exposed both in 2013-2014 and 2011-2014 has been analysed using Thermal Desorption Spectrometry (TDS). The deuterium (D) amounts obtained with TDS were compared with Ion Beam Analysis ...

Elsevier2019

On-chip mesoporous functionalized magnetic microspheres for protein sequencing by extended bottom-up mass spectrometry

Hubert Girault, Yury Tsybin, Liang Qiao, Natalia Gasilova, Kristina Srzentic

Limited amount and extreme concentration variability of proteomic-related samples require efficient analyte preconcentration and purification prior to the mass spectrometry (MS)-based analysis. Preferably, these steps should be coupled on-line with chosen ...

American Chemical Society2016