Publications associées à The road ahead in materials and technologies for volumetric 3D printing

Publications associées (17)

Bulk measurement of membrane permeability for random cyclic peptides in living cells to guide drug development

Nathan Moïse De Sadeleer, Christian Heinis, Alexander Lund Nielsen, Anne Sofie Luise Zarda, Rebecca Manuela Neeser

Cyclic peptides are attractive for drug discovery due to their excellent binding properties and the potential to cross cell membranes. However, by far not all cyclic peptides are cell permeable, and measuring or predicting their membrane permeability is no ...

Wiley2025

Significant reduction of cell invasiveness in nanoneedle insertion into a living cell with an electron-beam-deposited probe: impacts of probe geometry, speed and vibration

Marcos Penedo Garcia

Intracellular probing of living cells using atomic force microscopy (AFM) has advanced significantly, but it requires specially designed nanoprobes to achieve precision and minimize damage. The development of focused ion beam (FIB)-milled nanoprobes enable ...

2025

Automated Dual-Mode Cell Monitoring To Simultaneously Explore Calcium Dynamics and Contraction-Relaxation Kinetics within Drug-Treated Stem Cell-Derived Cardiomyocytes

Pierre Marquet, Gerardo Turcatti, Benjamin Rappaz, Bokyoung Kim

This manuscript proposes a new dual-mode cell imaging system for studying the relationships between calcium dynamics and the contractility process of cardiomyocytes derived from human-induced pluripotent stem cells. Practically, this dual-mode cell imaging ...

AMER CHEMICAL SOC2023

Dissecting centriole fate in C. elegans development

Nils Kalbfuss

The presence of centrioles is critical for fundamental cell and developmental processes, including polarity, migration and division. Although centrioles are present in most proliferating cells, they are thought to vanish after cell cycle exit in some diffe ...

EPFL2022

Cryogenic electron tomography to determine thermodynamic quantities for nanoparticle dispersions

Francesco Stellacci, Quy Ong Khac, Paulo Henrique Jacob Silva, Davide Demurtas, Xufeng Xu, Francesca Olgiati, Anna Murello, Ting Mao

Here we present a method to extract thermodynamic quantities for nanoparticle dispersions in solvents. The method is based on the study of tomograms obtained from cryogenic electron tomography (cryoET). The approach is demonstrated for gold nanoparticles ( ...

Royal Society of Chemistry2022

Computational Design of Auxetic Shells

Mina Konakovic Lukovic

Recent advances in material science and digital fabrication provide promising opportunities for product design, mechanical and biomedical engineering, robotics, architecture, art, and science. Engineered materials and personalized fabrication are revolutio ...

EPFL2019

Small-Molecule Fluorescent Probes for Live-Cell Super-Resolution Microscopy

Kai Johnsson, Lu Wang, Michelle Susanne Frei, Aleksandar Salim

Super-resolution fluorescence microscopy is a powerful tool to visualize biomolecules and cellular structures at the nanometer scale. Employing these techniques in living cells has opened up the possibility to study dynamic processes with unprecedented spa ...

AMER CHEMICAL SOC2019

Planar Optical Nanoantennas Resolve Cholesterol-Dependent Nanoscale Heterogeneities in the Plasma Membrane of Living Cells

Jürgen Brugger, Valentin Flauraud, Pamina Winkler

Optical nanoantennas can efficiently confine light into nanoscopic hotspots, enabling single-molecule detection sensitivity at biological relevant conditions. This innovative approach to breach the diffraction limit offers a versatile platform to investiga ...

2017

Transient Nanoscopic Phase Separation in Biological Lipid Membranes Resolved by Planar Plasmonic Antennas

Jürgen Brugger, Valentin Flauraud, Pamina Winkler

Nanoscale membrane assemblies of sphingolipids, cholesterol, and certain proteins, also known as lipid rafts, play a crucial role in facilitating a broad range of important cell functions. Whereas on living cell membranes lipid rafts have been postulated t ...

American Chemical Society2017

Complementarity of PALM and SOFI for super-resolution live-cell imaging of focal adhesions

Theo Lasser, Aleksandra Radenovic, Marcel Leutenegger, Daniel Pawel Szlag, Tomas Lukes, Hendrik Georges Deschout, Lely Feletti, Azat Sharipov

Live-cell imaging of focal adhesions requires a sufficiently high temporal resolution, which remains a challenge for super-resolution microscopy. Here we address this important issue by combining photoactivated localization microscopy (PALM) with super-res ...

Nature Publishing Group2016

Photosynthetic reaction centre/carbon nanotube bundle composites

László Forró, Arnaud Magrez, Endre Horvath, Dóra Fejes-Kajtár

Since their discovery, carbon nanotubes (CNTs) have attracted intense attention to broad range of potential applications. In contrast to the 1D isolated single-walled carbon nanotubes (SWCNT), 2D films or bundles made of thousands of tubes have been introd ...

Wiley-V C H Verlag Gmbh2014

Tracking NK1 receptor diffusion in the membrane of living cells. Effect of receptor activation

Horst Arthur Vogel, Joachim Piguet, Luc Veya

Springer2013

Fast three-dimensional imaging of gold nanoparticles in living cells with photothermal optical lock-in Optical Coherence Microscopy

Theo Lasser, Christian Santschi, Martin Villiger, Noelia Laura Bocchio, Arno Pino Bouwens, Corinne Berclaz, Joan Goulley, Christophe Pache

We introduce photothermal optical lock-in Optical Coherence Microscopy (poli-OCM), a volumetric imaging technique, which combines the depth sectioning of OCM with the high sensitivity of photothermal microscopy while maintaining the fast acquisition speed ...

Optical Society of America2012

Phase separation in binary eye lens protein mixtures

Giuseppe Foffi, Nicolas Dorsaz

Liquid–liquid phase separation occurs in young mammalian eye lenses and in concentrated solutions of isolated eye lens proteins, and has been linked to some forms of cataract. Here we study theoretically the protein compositions and cloud temperatures of t ...

2011

Covalent cell surface functionalization of human fetal osteoblasts for tissue engineering

Sandrine Gerber, Françoise Borcard, Corinne Scaletta, Aurélien Godinat, Davide Städler

The chemical functionalization of cell-surface proteins of human primary fetal bone cells with hydrophilic bioorthogonal intermediates was investigated. Toward this goal, chemical pathways were developed for click reaction-mediated coupling of alkyne deriv ...

2011

Live-Cell dSTORM of Cellular DNA Based on Direct DNA Labeling

Suliana Manley, Aleksandr Benke

We have implemented the super-resolution method of direct stochastic optical reconstruction microscopy (dSTORM) to image nuclear and mitochondrial DNA in living cells. We also demonstrate time-lapse imaging, all using a dye that associates directly with ce ...

Wiley-Blackwell2011

Characterization of the cytotoxicity and imaging properties of secondharmonic nanoparticles

Demetri Psaltis, Ye Pu, Rachel Grange, Chia-Lung Hsieh, Ye Pu

We develop second-harmonic nanoparticles as the contrast agents for cell imaging. Second-harmonic nanoparticles show promise as cell imaging probes due to their non-bleaching, non-blinking, and coherent signal. Nanoparticles of noncentrosymmetric crystal s ...

Spie-Int Soc Optical Engineering, Po Box 10, Bellingham, Wa 98227-0010 Usa2010