Correlative multicolor 3D SIM and STORM microscopy

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Publications associées (31)

Advanced Techniques in Optical Diffraction Tomography

Ahmed Bassam Sayed Ayoub Mohamed Emam

In this thesis, we study the 3 challenges described above. First, we study different reconstruction techniques and assess the fidelity of each reconstruction results by means of structured illumination and phase conjugation. By reconstructing the 3D refrac ...

EPFL2022

Multidisciplinary Investigation of the Gut-Brain Ecosystem in a Model of Alzheimer's Disease

Arielle Louise Planchette

The search for an understanding of the causal elements that lead to neurodegenerative diseases has motivated researchers for decades. Today, Alzheimer's disease is the most prevalent form of dementia and affects approximately fifty million people worldwide ...

EPFL2022

Pixel super-resolution with spatially entangled photons

Edoardo Charbon, Jiuxuan Zhao

Pixelation is common in quantum imaging systems and limit the image spatial resolution. Here, the authors introduce a pixel super-resolution approach based on measuring the full spatially-resolved joint probability distribution of spatially-entangled photo ...

Nature Portfolio2022

Diamond-Based Nanoscale Quantum Relaxometry for Sensing Free Radical Production in Cells

Hoda Shirzad, Mayeul Sylvain Chipaux

Diamond magnetometry makes use of fluorescent defects in diamonds to convert magnetic resonance signals into fluorescence. Because optical photons can be detected much more sensitively, this technique currently holds several sensitivity world records for r ...

WILEY-V C H VERLAG GMBH2022

Stain-free identification of cell nuclei using tomographic phase microscopy in flow cytometry

Demetri Psaltis, Joowon Lim, Daniele Pirone

The accurate identification of the three-dimensional quantitative shape of a cell nucleus is now possible without fluorescent staining by applying computational segmentation to refractive index tomograms recorded in the flow cytometry mode. ...

Nature Publishing Group2022

Single-molecule localization microscopy

Suliana Manley, Juliette Griffie

Single-molecule localization microscopy (SMLM) describes a family of powerful imaging techniques that dramatically improve spatial resolution over standard, diffraction-limited microscopy techniques and can image biological structures at the molecular scal ...

SPRINGERNATURE2021

Systematic Tuning of Rhodamine Spirocyclization for Super-resolution Microscopy

Kai Johnsson, Lu Wang

Rhodamines are the most important class of fluorophores for applications in live-cell fluorescence microscopy. This is mainly because rhodamines exist in a dynamic equilibrium between a fluorescent zwitterion and a nonfluorescent but cell-permeable spirocy ...

AMER CHEMICAL SOC2021

Towards quantum 3D imaging devices

Claudio Bruschini, Edoardo Charbon, Samuel Burri, Arin Can Ülkü, Paul Mos, Michael Alan Wayne

We introduce a new generation of 3D imaging devices based on quantum plenoptic imaging. Position-momentum entanglement and photon number correlations are exploited to provide a scan-free 3D image after post-processing of the collected light intensity signa ...

2021

High-Resolution Multi-Spectral Imaging With Diffractive Lenses and Learned Reconstruction

Oguzhan Fatih Kar, Can Deniz Bezek

Spectral imaging is a fundamental diagnostic technique with widespread application. Conventional spectral imaging approaches have intrinsic limitations on spatial and spectral resolutions due to the physical components they rely on. To overcome these physi ...

IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC2021

Diffraction Tomography from Single-Molecule Localization Microscopy: Numerical Feasibility

Michaël Unser, Thanh-An Michel Pham, Ferréol Arnaud Marie Soulez, Emmanuel Emilien Louis Soubies

Single-molecule localization microscopy (SMLM) is a fluorescence microscopy technique that achieves super-resolution imaging by sequentially activating and localizing random sparse subsets of fluorophores. Each activated fluorophore emits light that then s ...

IEEE2021

Parameter-free rendering of single-molecule localization microscopy data for parameter-free resolution estimation

Aleksandra Radenovic, Adrien Charles-François Raymond Descloux, Kristin Stefanie Grussmayer

Localization microscopy is a super-resolution imaging technique that relies on the spatial and temporal separation of blinking fluorescent emitters. These blinking events can be individually localized with a precision significantly smaller than the classic ...

2021

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