Correlative multicolor 3D SIM and STORM microscopy

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The search for an understanding of the causal elements that lead to neurodegenerative diseases has motivated researchers for decades. Today, Alzheimer's disease is the most prevalent form of dementia and affects approximately fifty million people worldwide ...

Women have made significant contributions to applied physics research and development, and their participation is vital to continued progress. Recognizing these contributions is important for encouraging increased involvement and creating an equitable envi ...

We study the presence within the worm Caenorhabditis elegans (C. elegans) of a fluorescent strain of the worm's bacterial food (Escherichia coli (E. coli) OP50) during early adulthood. Use of a microfluidic chip based on a thin glass coverslip substrate al ...

Pixelation is common in quantum imaging systems and limit the image spatial resolution. Here, the authors introduce a pixel super-resolution approach based on measuring the full spatially-resolved joint probability distribution of spatially-entangled photo ...

Fatigue damage in materials results in localized strain at the microstructural level. In many engineering components of the cooling circuits of nuclear power plants, where austenitic steels are used, the material experiences multiaxial cyclic loading, eith ...

Diamond magnetometry makes use of fluorescent defects in diamonds to convert magnetic resonance signals into fluorescence. Because optical photons can be detected much more sensitively, this technique currently holds several sensitivity world records for r ...

Innate immunity, the very first line of defence of our cells, relies on the detection of universal pathogen- or danger-associated signals to launch an inflammatory response. A crucial part of our innate immune system is based on the recognition of out-of-c ...

The accurate identification of the three-dimensional quantitative shape of a cell nucleus is now possible without fluorescent staining by applying computational segmentation to refractive index tomograms recorded in the flow cytometry mode. ...

Single-molecule localization microscopy (SMLM) describes a family of powerful imaging techniques that dramatically improve spatial resolution over standard, diffraction-limited microscopy techniques and can image biological structures at the molecular scal ...

X-ray micro-computed tomography (mu CT) was applied upon selected ophiolitic rock samples from various localities of the Vardar ophiolite outcrops in North Greece. Effectiveness of the mu CT application was evaluated through this case study by comparing re ...

Rhodamines are the most important class of fluorophores for applications in live-cell fluorescence microscopy. This is mainly because rhodamines exist in a dynamic equilibrium between a fluorescent zwitterion and a nonfluorescent but cell-permeable spirocy ...

We introduce a new generation of 3D imaging devices based on quantum plenoptic imaging. Position-momentum entanglement and photon number correlations are exploited to provide a scan-free 3D image after post-processing of the collected light intensity signa ...

Fluorescent D-amino acids (FDAAs) have previ-ously been developed to enable in situ highlighting of locations of bacterial cell wall growth. Most bacterial cells lie at the edge of the diffraction limit of visible light; thus, resolving the precise details ...

Super-resolution microscopy at angstrom precision could pave the way to optical structural biology in cells. ...

Spectral imaging is a fundamental diagnostic technique with widespread application. Conventional spectral imaging approaches have intrinsic limitations on spatial and spectral resolutions due to the physical components they rely on. To overcome these physi ...

Single-molecule localization microscopy (SMLM) is a fluorescence microscopy technique that achieves super-resolution imaging by sequentially activating and localizing random sparse subsets of fluorophores. Each activated fluorophore emits light that then s ...

Event-driven acquisition uses neural-network-based recognition of specific biological events to trigger switching between slow and fast super-resolution imaging, enriching the capture of interesting events with high spatiotemporal resolution. A common goal ...

Localization microscopy is a super-resolution imaging technique that relies on the spatial and temporal separation of blinking fluorescent emitters. These blinking events can be individually localized with a precision significantly smaller than the classic ...

In this thesis, we study the 3 challenges described above. First, we study different reconstruction techniques and assess the fidelity of each reconstruction results by means of structured illumination and phase conjugation. By reconstructing the 3D refrac ...

Correlative multicolor 3D SIM and STORM microscopy

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Multidisciplinary Investigation of the Gut-Brain Ecosystem in a Model of Alzheimer's Disease

The 2022 applied physics by pioneering women: a roadmap

High-resolution imaging and analysis of the intestinal bacterial load of Caenorhabditis elegans during early adulthood

Pixel super-resolution with spatially entangled photons

Microscopic Digital Image Correlation Analysis of Strain Distributions in Stainless Steel 316L Induced by Multiaxial Cyclic Loading

Diamond-Based Nanoscale Quantum Relaxometry for Sensing Free Radical Production in Cells

Tracking spatiotemporal localisation and interactions of the innate immune sensor STING

Stain-free identification of cell nuclei using tomographic phase microscopy in flow cytometry

Single-molecule localization microscopy

Effectiveness of X-ray micro-CT applications upon mafic and ultramafic ophiolitic rocks

Systematic Tuning of Rhodamine Spirocyclization for Super-resolution Microscopy

Towards quantum 3D imaging devices

Fluorescent D-Amino Acids for Super-resolution Microscopy of the Bacterial Cell Wall

Optical microscopy gets down to angstroms

High-Resolution Multi-Spectral Imaging With Diffractive Lenses and Learned Reconstruction

Diffraction Tomography from Single-Molecule Localization Microscopy: Numerical Feasibility

Event-driven acquisition for content-enriched microscopy

Parameter-free rendering of single-molecule localization microscopy data for parameter-free resolution estimation

Advanced Techniques in Optical Diffraction Tomography