Super-resolution imaging of multiple cells by optimized flat-field epi-illumination

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Background: Fetal brain MRI is increasingly used to complement ultrasound imaging. Images are processed using complex super-resolution reconstruction pipelines, which may bias biometric and volumetric measurements. Objective: To assess the consistency of 2 ...

The interplay of synthetic DNA nanostructures and lipid membranes is an exciting frontier in bioengineering, with the potential to transform synthetic biology and nanomedicine. However, the fundamental principles underlying their interactions-particularly ...

Magnetic resonance spectroscopic imaging (MRSI) enables the simultaneous noninvasive acquisition of MR spectra from multiple spatial locations inside the brain. Although 1H-MRSI is increasingly used in the human brain, it is not yet widely applied in the p ...

Neurodegenerative and neuroinflammatory disorders often involve complex pathophysiological mechanisms that are â to this date â only partially understood. A more comprehensive understanding of those microstructural processes and their characterization ...

The World Health Organization highlights the urgent need to address the global threat posed by antibiotic-resistant bacteria. Efficient and rapid detection of bacterial response to antibiotics and their virulence state is crucial for the effective treatmen ...

Time-domain solutions of Maxwell’s equations in homogeneous and isotropic media are paramount to studying transient or broadband phenomena. However, analytical solutions are generally unavailable for practical applications, while numerical solutions are co ...

Ribosomes translate the genetic code into proteins. Recent technical advances have facilitated in situ structural analyses of ribosome functional states inside eukaryotic cells and the minimal bacterium Mycoplasma. However, such analyses of Gram-negative b ...

Optical microscopy is an essential tool for biologists, who are often faced with the need to overcome the spatial and temporal resolution limitations of their devices to capture finer details. As upgrading imaging hardware is expensive, computational metho ...

Fluorescence confocal laser-scanning microscopy (LSM) is one of the most popular tools for life science research. This popularity is expected to grow thanks to single-photon array detectors tailored for LSM. These detectors offer unique single-photon spati ...

Antibiotic resistance is nowadays a major public health issue. Rapid antimicrobial susceptibility tests (AST) are one of the options to fight this deadly threat. Performing AST with single-cell sensitivity that is rapid, cheap, and widely accessible, is ch ...

The proliferation of microscopy methods for live-cell imaging offers many new possibilities for users but can also be challenging to navigate. The prevailing challenge in live-cell fluorescence microscopy is capturing intra-cellular dynamics while preservi ...

The evolution of the 3D morphology is at the center of many relevant biological processes ranging from cellular differentiation to cancer invasion and metastasis. Microscopy techniques, such as electron microscopy (EM), super-resolution (SR) optical micros ...

In this thesis, we explored on-chip high-resolution imaging of the fate of intestinal bacteria and bacterial products in Caenorhabditis elegans (C. elegans). In the first part, we carried out high-resolution z-stack fluorescent imaging of Red Fluorescent P ...

Quantum ghost imaging can be an important tool in making optical measurements. One of the most useful aspects of ghost imaging is the unique ability to correlate two sets of independently collected information. We aim to use the principles of ghost imaging ...

Noninvasive tracking of biochemical processes in the body is paramount in diagnostic medicine. Among the leading techniques is spectroscopic magnetic resonance imaging (MRI), which tracks metabolites with an amplified (hyperpolarized) magnetization signal ...

We study the presence within the worm Caenorhabditis elegans (C. elegans) of a fluorescent strain of the worm's bacterial food (Escherichia coli (E. coli) OP50) during early adulthood. Use of a microfluidic chip based on a thin glass coverslip substrate al ...

Bacteria often engage in social interactions with neighbouring bacteria. Ecosystems which are subjected to social interactions have been widely studied in well mixed settings such as test tubes, allowing us to identify the cellular components contributing ...

Cell segmentation is a critical step for quantitative single-cell analysis in microscopy images. Existing cell segmentation methods are often tailored to specific modalities or require manual interventions to specify hyper-parameters in different experimen ...

Polo-like kinase (Plk4) is a serine/threonine-proteinkinase thatis essential for biogenesis of the centriole organelle and is enrichedat centrioles. Herein, we introduce Cen-TCO, a chemical probe basedon the Plk4 inhibitor centrinone, to image Plk4 and cen ...

Atomic force microscopy (AFM) is a widely used imaging tool for obtaining a variety of information for a range of samples. Although it was initially intended to serve as a method of observing very flat solid surfaces, its use expanded into several other fi ...

Super-resolution imaging of multiple cells by optimized flat-field epi-illumination

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Biometry and volumetry in multi-centric fetal brain magnetic resonance imaging: assessing the bias of super-resolution reconstruction

Electrostatic and Hydrophobic Mediation in DNA/Lipid Interactions: Toward the Design of Tailored DNA/Membrane Interfaces

Fast High-Resolution Metabolite Mapping in the rat Brain Using 1H-FID-MRSI at 14.1 T

Development and clinical validation of computational imaging biomarkers for neurodegenerative diseases

Machine learning method for the classification of the state of living organisms' oscillations

The time-domain Cartesian multipole expansion of electromagnetic fields

Bactericidal effect of tetracycline in E. coli strain ED1a may be associated with ribosome dysfunction

Practical computational imaging by use of spatiotemporal light modulation: from simulations to applications in biological microscopy

Compact and effective photon-resolved image scanning microscope

Simple optical nanomotion method for single-bacterium viability and antibiotic response testing

Live-cell imaging powered by computation

Time-Resolved Scanning Ion Conductance Microscopy and Single-Molecule Spectroscopy

Microfluidic devices for automated analysis of ingested bacteria in the Caenorhabditis elegans intestine

Ghost imaging using two SPAD array detectors: a parameter study towards the realization of a 3D quantum microscope

Long-lived enhanced magnetization-A practical metabolic MRI contrast material

High-resolution imaging and analysis of the intestinal bacterial load of Caenorhabditis elegans during early adulthood

The Effects of Spatial Organization and Fluid Flow on the Social Interactions in Microbial Communities

The multimodality cell segmentation challenge: toward universal solutions

Chemical Probe for Imaging of Polo-like Kinase 4 and Centrioles

Advancements in Nanomechanical Characterization and Biomolecular Imaging with Atomic force Microscopy