Beyond the lateral resolution limit by phase imaging

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Quantitative Phase Imaging techniques such as DHM have emerged recently in life sciences and can be aimed at monitoring and quantifying non-invasively dynamic cellular processes modifying cell morphology and/or content. Concretely, the DHM phase signal depends on two cell parameters: cell thickness and integral refractive index. Consequently, due to its dual origin, the interpretation of the phase signal variations remain difficult. Since a net water flux across the cell membrane causes a variation of both

Combinatorial high-vacuum chemical vapor deposition (HV-CVD) was used to identify the conditions required to obtain hafnium-doped lithium niobate thin films on sapphire {001} substrates. Niobium tetraethoxydimethylaminoethoxide (Nb(OEt)(4)(dmae)), lithium tert-butoxide (Li(OBut)), and hafnium tert-butoxide (Hf(OBut)(4)) were used as precursors. X-ray diffraction (XRD) and transmission electron microscopy (TEM) indicated that a single phase of textured {001} Hf-doped lithium niobate film was obtained under c

Digital Holographic Microscopy (DHM) has been used to investigate the spontaneous cell membrane fluctuations (CMF) of the Red Blood Cell. DHM as an interferometric technique is able to accurately provide the wavefront deformation induced by a transparent specimen, including living cells in a transmission configuration. From a numerical reconstruction of a single hologram, quantitative phase contrast images are obtained. The local phase shift is proportional to the specimen thickness with accuracy of 5-10 nm

A dual mode microscope is developed to study morphological evolution of mouse myoblast cells under simulated microgravity in real time. Microscope operates in Digital Holographic Microscopy (DHM) and widefield epifluorescence microscopy modes in a time sequential basis. DHM provides information on real time cellular morphology. EGFP transfected actin filaments in mouse myoblast cells function as the reporter for the fluorescence microscopy mode. Experimental setup is fixed in the RPM to observe microgravity

(001)-epitaxial SrTiO3 films were grown on (La, Sr)(Al, Ta)O-3 substrates for investigating the impact of biaxial strain on the antiferrodistortive (AFD) structural phase transition in SrTiO3. The films were fully constrained by the substrates, which resulted in the large in-plane compressive strain of -0.9%. The AFD transition temperature estimated using temperature controlled x-ray diffraction and transmission electron microscope was over 150K higher than the theoretical prediction, but rather supported t

A novel imaging method employing the different kinetics of the energetic states of fluorescent markers is presented, where the contrast depends on the triplet kinetics. An attractive feature of triplet states is their long lifetime, rendering them highly sensitive to the immediate environment of their fluorescent host molecules. They typically have a lifetime of the order of ~10us to 10ms, which is 3-6 orders of magnitude longer than the lifetimes of fluorescent singlet states. The concept exploits characte

Digital holographic microscopy (DHM) is a technique that allows obtaining, from a single recorded hologram, quantitative phase image of living cell with interferometric accuracy. Specifically the optical phase shift induced by the specimen on the transmitted wave front can be regarded as a powerful endogenous contrast agent, depending on both the thickness and the refractive index of the sample. We have recently proposed a new and efficient decoupling procedure allowing to directly obtain separate measureme

Digital Holographic Microscopy (DHM) is a powerful imaging technique allowing, from a single amplitude image (amplitude and phase), reflected acquisition (hologram), the reconstruction of the entire complex wave front by or transmitted through an object. Because holography is an interferometric technique, the reconstructed phase leads to a sub-wavelength axial accuracy (below lambda/100). Nevertheless, this accuracy is difficult to obtain from a single hologram. Indeed, the reconstruction process consisting

Stencil lithography is a surface patterning technique that relies on the local deposition of material through miniaturized shadow mask membranes. This method has been used since many years in various implementations for the formation of patterns, mainly structured thin metal films, in situations when lithography equipment is not available or when the surfaces don’t allow the harsh process steps typically involved in photolithography such as spin coating of photoresist, high temperature baking, development,

A versatile method for parallel nanoscale structures fabrication and patterning, using a combination of stencil-mask and pulsed-laser deposition (PLD) techniques is presented. A miniature shadow-mask with nano-apertures in a very thin microfabricated membrane (nanostencil) was separately manufactured and then, mechanically attached to the substrate of choice. In this way, complex oxides such as BaTiO3, BiFeO3 and PZT were deposited directly through the stencil’s holes onto various substrates (Si, Pt coated

An intelligent pinhole for confocal microscopy consisting of four position controlled blades forming a rectangular aperture from 3x3μm to 500 x 500 μm was built. This aperture can be positioned without drift under computer control in a positioning range with a sub-micrometer precision. Each blade is suspended by an elastic hinges and moved by a linear magnetic actuator. An integrated position transducer with a reproducibility of better than 40 nm allows a settling time of less than 75 ms. The whole pinhole

Beyond the lateral resolution limit by phase imaging

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Dual-wavelength Digital Holography for quantification of cell volume and integral refractive index (RI)

Combinatorial High-Vacuum Chemical Vapor Deposition of Textured Hafnium-Doped Lithium Niobate Thin Films on Sapphire

Exploring red blood cell membrane dynamics with digital holographic microscopy

Dual-mode digital holographic and fluorescence microscopy for the study of morphological changes in cells under simulated microgravity

Antiferrodistortive Structural Phase Transition in Compressively-Strained Epitaxial SrTiO3 Film Grown on (La, Sr)(Al, Ta)O-3 Substrate

Triplet imaging contrast by modulated excitation

Erythrocytes volume and refractive index measurement with a digital holographic microscope

Automatic procedure for aberrations compensation in digital holographic microscopy

Nanostencil Lithography - Quick & Clean: Towards a reliable scalable nanopatterning method

Nanostenciling: a Versatile Approach for Surfaces Patterning

Intelligent Pinhole with Sub-Micrometer Resolution