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Novel Concepts for Functional High Resolution Microscopy

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Low-level light detection with high spatial and timing accuracy is a growing area of interest by virtue of applications such as light detection and ranging (LiDAR), biomedical imaging, time-resolved Raman spectroscopy, and quantum applications. Single-phot ...

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Exploring Novel Modalities for Optical Diffraction Tomography

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In the past decade, optical diffraction tomography has gained a lot of attention for its ability to create label-free three-dimensional (3D) images of the refractive index distribution of biological samples using scattered fields measured through holograph ...

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Functional neuroimaging options for tinnitus

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Progress and prospects in nonlinear extreme-ultraviolet and X-ray optics and spectroscopy

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Free-electron lasers and high-harmonic-generation table-top systems are new sources of extreme-ultraviolet to hard X-ray photons, providing ultrashort pulses that are intense, coherent and tunable. They are enabling a broad range of nonlinear optical and s ...

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Time-Resolved Scanning Ion Conductance Microscopy and Single-Molecule Spectroscopy

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The evolution of the 3D morphology is at the center of many relevant biological processes ranging from cellular differentiation to cancer invasion and metastasis. Microscopy techniques, such as electron microscopy (EM), super-resolution (SR) optical micros ...

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A chip-scale second-harmonic source via self-injection-locked all-optical poling

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Second-harmonic generation allows for coherently bridging distant regions of the optical spectrum, with applications ranging from laser technology to self-referencing of frequency combs. However, accessing the nonlinear response of a medium typically requi ...

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Structural diversity of photoswitchable sphingolipids for optodynamic control of lipid microdomains

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Sphingolipids are a structurally diverse class of lipids predominantly found in the plasma membrane of eukaryotic cells. These lipids can laterally segregate with other rigid lipids and cholesterol into liquid-ordered domains that act as organizing centers ...

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Hybrid lithium niobate-on-silicon nitride platform for mid-IR supercontinuum generation

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Integrated optics has shown itself very convenient for exploiting nonlinear processes as it results in high confinement factor, freedom of dispersion engineering and compactness. However, the choice of materials is crucial for the development of nonlinear ...

SPIE-INT SOC OPTICAL ENGINEERING2023

In situ monitoring of femtosecond laser-induced modifications in dielectrics

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Over the last decades, the progress made in the generation of laser pulses shorter than a picosecond (10^-12 s) has allowed us to reach extreme optical power intensities exceeding 10^15 W cm^-2. This tremendous power has triggered an abundance of original ...

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We investigate and characterize the emergence of finite-component dissipative phase transitions (DPTs) in nonlinear photon resonators subject to n-photon driving and dissipation. Exploiting a semiclassical approach, we derive general results on the occurre ...

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We propose an image-based elastography method to measure the heterogeneous stiffness inside a cell and its nucleus. It uses a widely accessible setup consisting of plate compression imaged with fluorescence microscopy. Our framework recovers a spatial map ...

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Nonlinear optical frequency conversion is of fundamental importance in photonics and underpins countless of its applications: Sum- and difference-frequency generation in media with quadratic nonlinearity permits reaching otherwise inaccessible wavelength r ...

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Optical microscopy is an essential tool for biologists, who are often faced with the need to overcome the spatial and temporal resolution limitations of their devices to capture finer details. As upgrading imaging hardware is expensive, computational metho ...

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High throughput wide field second harmonic imaging of giant unilamellar vesicles

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Cell-sized giant unilamellar vesicles (GUVs) are an ideal tool for understanding lipid membrane structure and properties. Label-free spatiotemporal images of their membrane potential and structure would greatly aid the quantitative understanding of membran ...

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Background Innate immune cells play a crucial role in responding to microbial infections, but their improper activation can also drive inflammatory disease. For this reason, their activation state is governed by a multitude of factors, including the metabo ...

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Magnetic resonance spectroscopy is a powerful, non-invasive, quantitative imaging technique that allows for the measurement of brain metabolites that has demonstrated utility in diagnosing and characterizing a broad range of neurological diseases. Its impa ...

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