Proof-of-concept analytical instrument for label-free optical deconvolution of protein species in a mixture

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Publications associées (32)

QUAREP-LiMi: A community-driven initiative to establish guidelines for quality assessment and reproducibility for instruments and images in light microscopy

Andrea Bassi, Antje Keppler, Arne Seitz

A modern day light microscope has evolved from a tool devoted to making primarily empirical observations to what is now a sophisticated , quantitative device that is an integral part of both physical and life science research. Nowadays, microscopes are fou ...

WILEY2021

Biophysical applications of correlative scanning probe and super-resolution microscopy

Vytautas Navikas

Imaging live cells in their native environment is crucial for the understanding of complex biological phenomena. Modern optical microscopy methods such as fluorescence super-resolution microscopy are increasingly combined with complementary, label-free tec ...

EPFL2021

Correlative 3D microscopy of single cells using super-resolution and scanning ion-conductance microscopy

Aleksandra Radenovic, Adrien Charles-François Raymond Descloux, Georg Fantner, Samuel Mendes Leitão, Kristin Stefanie Grussmayer, Barney Frederick Drake, Vytautas Navikas

High-resolution live-cell imaging is necessary to study complex biological phenomena. Modern fluorescence microscopy methods are increasingly combined with complementary, label-free techniques to put the fluorescence information into the cellular context. ...

Nature Research2021

Weakly Supervised Deep Learning Methods for Biomicroscopy

Adrian Shajkofci

Optical microscopy, an invaluable tool in biology and medicine to observe and quantify cellular function, organ development, or disease mechanisms, requires constant trade-offs between spatial, temporal, and spectral resolution, invasiveness, acquisition t ...

EPFL2021

QUAREP-LiMi: a community endeavor to advance quality assessment and reproducibility in light microscopy

Arne Seitz

The community-driven initiative Quality Assessment and Reproducibility for Instruments & Images in Light Microscopy (QUAREP-LiMi) wants to improve reproducibility for light microscopy image data through quality control (QC) management of instruments and im ...

2021

Quantum dynamics in individual surface spins

Tobias Bilgeri

This thesis presents a combined experimental and theoretical study of the classical and quantum magnetization dynamics in single magnetic adatoms and molecules, and on the classical and quantum coherent control thereof. First, a detailed description of t ...

EPFL2021

Additive manufacturing of a precious bulk metallic glass

Jamasp Jhabvala, Güven Kurtuldu, Antonia Neels, Roland Logé, Navid Sohrabi, Annapaola Parrilli

For the first time, a high-density amorphous and crack-free bulk metallic glass (BMG) based on a precious metal (PdCuNiP) was produced via additive manufacturing (AM). Laser powder-bed fusion (LPBF) was used for the fabrication of the samples, and led to a ...

2021

Structured light generation by refractive-diffractive micro optical array illuminators

Maryam Yousefi

Structured light generation having broad applications in different optical fields, is the topic of this thesis. Our structured light generation strategy is based on applying periodic microoptical elements at the refraction-diffraction limit, under a focuse ...

EPFL2021

Hardware and Software Interfaces Design for Multi-Panel Electrochemical Sensors

Mandresy Ivan Ny Hanitra

The exponential growth of wearable healthcare devices market is fostered by the internetof- things (IoT) era. Connected smart biosensors enable a decentralized healthcare that does not constrain the user to be in a medical facility to get a real-time insig ...

EPFL2021

Investigating Tissue Mechanics in vitro Using Untethered Soft Robotic Microdevices

Mahmut Selman Sakar, Raquel Filipa Penacho Parreira, Ece Özelçi

This paper presents the design, fabrication, and operation of a soft robotic compression device that is remotely powered by laser illumination. We combined the rapid and wireless response of hybrid nanomaterials with state-of-the-art microengineering techn ...

2021

Large-Format Time-Gated SPAD Cameras for Real-Time Phasor-Based FLIM

Arin Can Ülkü

Fluorescence lifetime imaging microscopy (FLIM) is an imaging modality often used to monitor biochemical properties of a cell or a tissue. In addition to conventional fluorescence microscopy features, such as selective labeling and non-invasiveness, FLIM e ...

EPFL2021

Polarization-sensitive optics diffraction tomography

Demetri Psaltis, Ahmed Ayoub, Joowon Lim, Elizabeth Elena Antoine, Amirhossein Saba Shirvan

Polarization of light has been widely used as a contrast mechanism in two-dimensional (2D) microscopy and also in some three-dimensional (3D) imaging modalities. In this paper, we report the 3D tomographic reconstruction of the refractive index (RI) tensor ...

2021

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