Processing of fluorescence angiograms for the quantification of vascular effects induced by anti-angiogenic agents in the CAM model

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All organisms live within a given thermal range, but little is known about the mechanisms setting the limits of this range. We uncovered cellular features exhibiting signature changes at thermal limits in Caenorhabditis elegans embryos. These included chan ...

Angiogenesis is the process by which new blood vessels arise from existing ones by the budding out of endothelial cell capillaries from the luminal side of blood vessels. Blood vessel formation is essential for organ development during embryogenesis and is ...

Biomechanical forces, such as fluid shear stress, govern multiple aspects of endothelial cell biology. In blood vessels, disturbed flow is associated with vascular diseases, such as atherosclerosis, and promotes endothelial cell proliferation and apoptosis ...

Axis formation and allocation of pluripotent progenitor cells to the germ layers are governed by the TGF-β-related Nodal precursor and its secreted proprotein convertases (PCs) Furin and Pace4. However, when and where Furin and Pace4 first become active ha ...

Cell polarity underlies many aspects of metazoan development and homeostasis, and relies notably on a set of PAR proteins located at the cell cortex. How these proteins interact in space and time remains incompletely understood. We performed a quantitative ...

The early Caenorhabditis elegans embryo is an attractive model to investigate evolutionarily conserved cellular mechanisms. However, there is a paucity of automated methods to gather quantitative information with subcellular precision in this system. We de ...

The chicken chorioallantoic membrane (CAM) is a simple, highly vascularized extraembryonic membrane, which performs multiple functions during embryonic development, including but not restricted to gas exchange. Over the last two decades, interest in the CA ...

This paper reports on a new microfluidic device for temperature stimulation of cell in in-vitro culture. Micro- electrodes in a meander shape are embedded into the microfluidic channels to generate either a temperature gradient through the culture chamber ...

Synergy in the downstream signaling pathways of the vascular endothelial growth factor receptor-2 (VEGFR-2) and the integrin alpha v beta 3 is critical for blood vessel formation. Thus, agents that activate both receptors could possess efficient pro-angiog ...

Processing of fluorescence angiograms for the quantification of vascular effects induced by anti-angiogenic agents in the CAM model

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Cellular hallmarks reveal restricted aerobic metabolism at thermal limits

VE-statin/egfl7 expression in endothelial cells is regulated by a distal enhancer and a proximal promoter under the direct control of Erg and GATA-2

FOXC2 and fluid shear stress stabilize postnatal lymphatic vasculature

Imaging proprotein convertase activities and their regulation in the implanting mouse blastocyst

Quantitative Analysis and Modeling Probe Polarity Establishment in C. elegans Embryos

ASSET: A robust algorithm for the automated segmentation and standardization of early Caenorhabditis elegans embryos

The chicken chorioallantoic membrane model in biology, medicine and bioengineering

Temperature gradient stimulation for cell division in C. Elegans Embryos on chip

The promotion of endothelial cell attachment and spreading using FNIII10 fused to VEGF-A(165)