Dynamic disorder in horseradish peroxidase observed with total internal reflection fluorescence correlation spectroscopy

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Publications associées (32)

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A fluorescence correlation contrast is a straightforward approach to super-resolution imaging. Combining a SPAD array with a novel detection scheme (ISM), we obtain images with up to x4 times resolution enhancement. ...

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Biophysical applications of correlative scanning probe and super-resolution microscopy

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Imaging live cells in their native environment is crucial for the understanding of complex biological phenomena. Modern optical microscopy methods such as fluorescence super-resolution microscopy are increasingly combined with complementary, label-free tec ...

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Correlative 3D microscopy of single cells using super-resolution and scanning ion-conductance microscopy

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High-resolution live-cell imaging is necessary to study complex biological phenomena. Modern fluorescence microscopy methods are increasingly combined with complementary, label-free techniques to put the fluorescence information into the cellular context. ...

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Diffraction Tomography from Single-Molecule Localization Microscopy: Numerical Feasibility

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Single-molecule localization microscopy (SMLM) is a fluorescence microscopy technique that achieves super-resolution imaging by sequentially activating and localizing random sparse subsets of fluorophores. Each activated fluorophore emits light that then s ...

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Systematic Tuning of Rhodamine Spirocyclization for Super-resolution Microscopy

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Rhodamines are the most important class of fluorophores for applications in live-cell fluorescence microscopy. This is mainly because rhodamines exist in a dynamic equilibrium between a fluorescent zwitterion and a nonfluorescent but cell-permeable spirocy ...

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Estimating Nonplanar Flow from 2D Motion-blurred Widefield Microscopy Images via Deep Learning

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Optical flow is a method aimed at predicting the movement velocity of any pixel in the image and is used in medicine and biology to estimate flow of particles in organs or organelles. However, a precise optical flow measurement requires images taken at hig ...

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Single-molecule localization microscopy (SMLM) describes a family of powerful imaging techniques that dramatically improve spatial resolution over standard, diffraction-limited microscopy techniques and can image biological structures at the molecular scal ...

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Defects in solid-state systems can be both detrimental, deteriorating the quality of materials, or desired, thanks to the novel functionality they bring. Optically active point defects, producing fluorescent light, are a great example of the latter. Natura ...

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Organisation and dynamics of mitochondria and their RNA

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The organisation of molecules into dynamic cells, and collaboration of many of those cells over a billion years led to the evolution of human life. During the last century, biologists then began to unravel the marvels of cellular organisation with ever inc ...

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Fundamental bounds on the precision of iSCAT, COBRI and dark-field microscopy for 3D localization and mass photometry

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Interferometric imaging is an emerging technique for particle tracking and mass photometry. Mass or position are estimated from weak signals, coherently scattered from nanoparticles or single molecules, and interfered with a co-propagating reference. In th ...

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Structural and analytical identifications of biomolecules by cold ion spectroscopy of non-covalent complexes

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Non-covalent interactions are ubiquitous in nature. The diversity and functionality of these interactions are employed by nature as a universal tool for building the molecular mechanism of life. Although weak, these interactions play a key role in both for ...

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Direct observation of water-mediated single-proton transport between hBN surface defects

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Super-resolution microscopy of defects in a two-dimensional material unveils the transport of single proton charges at solid/water interfaces. Aqueous proton transport at interfaces is ubiquitous and crucial for a number of fields, ranging from cellular tr ...

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