Tailoring the Hybridization Density of DNA Biosensors through Tunable Surface Functionalization

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The adaptation of organisms to their environment depends on the innovative potential inherent to genetic variation. In complex organisms such as mammals, processes like development and immunity require tight gene regulation. Complex forms emerge more often ...

Gene regulation is mediated by site-specific DNA-binding proteins or transcription factors (TFs), which form protein complexes at regulatory loci either to activate or repress the expression of a target gene. The study of the dynamic properties of these re ...

Recombinant proteins are gaining in importance for therapeutic applications. The proteins are expressed in stable cell lines, within which recombinant DNA has incorporated into the host cells genome. Identification and isolation of extremely high producers ...

The COVID-19 pandemic has highlighted the necessity to develop fast, highly sensitive and selective virus detection methods. Surface-based DNA-biosensors are interesting candidates for this purpose. Functionalization of solid substrates with DNA must be pr ...

The invention provides a method for quantifying the number of target entities that were successfully encapsulated into a microfluidic compartment such as a microfluidic droplet. The invention is predicated upon a continuous quantifiable detectable signal c ...

Increasing interest in understanding fundamental biological processes, among which aging and nutrition, has led to the study of simple and powerful model organisms, as the round-worm Caenorhabditis elegans (C. elegans). The nematode, selected by Sydney Bre ...

Fluctuations and deterioration in environmental conditions potentially have a phenotypic impact that extends over generations. Transgenerational epigenetics is the defined term for such intergenerational transient inheritance without an alteration in the D ...

In this study we present a novel microfluidic hydrodynamic trapping device to probe the cell–cell interaction between all cell samples of two distinct populations. We have exploited an hydrodynamic trapping method using microfluidics to immobilize a batch ...

Shewanella oneidensis produces an extensive electron transfer network that results in metabolic flexibility. A large number of c-type cytochromes are expressed by S. oneidensis and these function as the fundamental electron transport chain proteins. Althou ...

A boundary-fitted moving mesh scheme is presented for the simulation of two-phase flow in two-dimensional and axisymmetric geometries. The incompressible Navier-Stokes equations are solved using the finite element method, and the mini element is used to sa ...

The nematode Caenorhabditis elegans is an attractive model organism, owing notably to its short life cycle, genetic tractability, and optical transparency facilitating microscopic observation. This thesis deals with the realization of technological tools f ...

DNA encrypts the composition of the cellular material that is synthesized through transcription and translation. Never-theless, gene regulation mechanisms determine the final amount of transcribed and translated material. Transcription factors (TF) are a c ...

With the advent of high-throughput and genome-wide screening initiatives, there is a need for improved methods for cell-based assays. Current approaches require expensive equipment, rely on large-scale culturing formats not suited for small or rare sample ...

The integration of nanoscale sensors with microfluidic platforms is a powerful tool for the design of robust biosensing devices that present high reliability and the advantage of a quick data acquisition. In addition, microfluidic based lab-on-a-chip senso ...

Chromogenic immunohistochemistry (IHC) is omnipresent in cancer diagnosis, but has also been criticized for its technical limit in quantifying the level of protein expression on tissue sections, thus potentially masking clinically relevant data. Shifting f ...

Electric fields can be effectively used to sense, manipulate, and move particles in lab-on-a-chip devices. Nevertheless, the throughput of such devices is a critical issue, which can be effectively improved by increasing the height of the microchannels. Fo ...

Bicyclic peptides are an attractive molecule format for the development of therapeutics and research tools. Our laboratory is specialized on the development of bicyclic peptide ligands by phage display. In brief, libraries of randompeptides each containing ...

Fluorescence in situ hybridization (FISH) is a powerful technique for evaluating the HER2 gene status in breast cancer specimen (1). However, most of FISH assays currently used in clinical laboratories are expensive and require long experimental time, up t ...

Optical imaging emerges as a vital component of the various techniques needed to meet the stringent requirements of modern bioanalysis and bioimaging. Lanthanide luminescent bioprobes (LLBs) have greatly contributed to this field during the past 35 years b ...

Polydimethylsiloxane (PDMS) and SU-8 are currently two very commonly used polymeric materials in the microfluidics field for biological applications. However; there is a pressing need to find a simple, reliable, irreversible bonding method between these tw ...

Tailoring the Hybridization Density of DNA Biosensors through Tunable Surface Functionalization

Chattez avec Graph Search

Meeting our Makers: Uncovering the cis-regulatory activity of transposable elements using statistical learning

DNA-centered approaches to characterize regulatory protein–DNA interaction complexes

Establishment of recombinant mammalian cell lines : new approaches

Achieving high hybridization density at DNA biosensor surfaces using branched spacer and click chemistry

Quantification of successful encapsulation into microfluidic compartments

Microfluidic culture platforms for C. elegans bacterial interaction and digestion studies at single-organism resolution

The Detection of Early Epigenetic Inheritance of Mitochondrial Stress in C. Elegans with a Microfluidic Phenotyping Platform

Microfluidic device performing on flow study of serial cell–cell interactions of two cell populations

The small RNA RyhB is a regulator of cytochrome expression in Shewanella oneidensis

Interface-fitted moving mesh method for axisymmetric two-phase flow in microchannels

Lab-on-a-chip technologies for manipulation and imaging of C. elegans worms and embryos

Modeling and analysis of parts and devices of genetic regulation

Microfluidic platforms for high-throughput mammalian cell printing, transfection, and dosage-dependent studies

Memristive Biosensors Integration With Microfluidic Platform

Continuous quantification of HER2 expression by microfluidic precision immunofluorescence estimates HER2 gene amplification in breast cancer

Metal-Coated SU-8 Structures for High-Density 3-D Microelectrode Arrays

Screening of structurally diverse bicyclic peptide libraries by phage display

Extra short incubation microfluidic assisted – fluorescence in situ hybridization (ESIMA-FISH)

Lanthanide light for biology and medical diagnosis

A Simple and Reliable PDMS and SU-8 Irreversible Bonding Method and Its Application on a Microfluidic-MEA Device for Neuroscience Research