Time-domain optical coherence tomography with digital holographic microscopy

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In unfocused ultrasound imaging, a delay-and-sum algorithm is commonly used to reconstruct one image per emission. When multiple emissions are performed, individual images can be combined by coherent compounding to improve image quality. Alternative method ...

Correlative light and electron microscopy (CLEM) combines light microscopy, for identifying a target via genetic labels, dyes, antibodies and morphological features, with electron microscopy, for analyzing high-resolution subcellular ultrastructures. Here, ...

Amyloid aggregation is associated with neurodegenerative disease and its modulation is a focus of drug development. We developed a chemical proteomics pipeline to probe the mechanism of action of anti-amyloidogenic compounds. Our approach identifies putati ...

The proliferation of microscopy methods for live-cell imaging offers many new possibilities for users but can also be challenging to navigate. The prevailing challenge in live-cell fluorescence microscopy is capturing intra-cellular dynamics while preservi ...

Phase imaging is widely used in biomedical imaging, sensing, and material characterization, among other fields. However, direct imaging of phase objects with subwavelength resolution remains a challenge. Here, we demonstrate subwavelength imaging of phase ...

Significance: Fluorescence guidance is used clinically by surgeons to visualize anatomical and/or physiological phenomena in the surgical field that are difficult or impossible to detect by the naked eye. Such phenomena include tissue perfusion or molecula ...

Objectives To evaluate the performance of automatic deep learning (DL) algorithm for size, mass, and volume measurements in predicting prognosis of lung adenocarcinoma (LUAD) and compared with manual measurements. ...

Polo-like kinase (Plk4) is a serine/threonine-proteinkinase thatis essential for biogenesis of the centriole organelle and is enrichedat centrioles. Herein, we introduce Cen-TCO, a chemical probe basedon the Plk4 inhibitor centrinone, to image Plk4 and cen ...

Imaging live cells in their native environment is crucial for the understanding of complex biological phenomena. Modern optical microscopy methods such as fluorescence super-resolution microscopy are increasingly combined with complementary, label-free tec ...

Optical microscopy is an essential tool for biologists, who are often faced with the need to overcome the spatial and temporal resolution limitations of their devices to capture finer details. As upgrading imaging hardware is expensive, computational metho ...

We introduce a new generation of 3D imaging devices based on quantum plenoptic imaging. Position-momentum entanglement and photon number correlations are exploited to provide a scan-free 3D image after post-processing of the collected light intensity signa ...

Designing architectural façades that allow sufficient daylight to create visually comfortable and pleasant environments is a challenging aspect of building design. It requires accounting for visual comfort and discomfort glare risks and understanding the f ...

Visualization of organelles and their interactions with other features in the native cell remains a challenge in modern biology. We have introduced cryo-scanning transmission electron tomography (CSTET), which can ac-cess 3D volumes on the scale of 1 micro ...

PURPOSE: To determine and compare the origin of the external surface reflections produced by commonly used intraocular lenses (IOLs). ...

Positron emission tomography is a nuclear imaging technique well known for its use in oncology for cancer diagnosis and staging. A PET scanner is a complex machine which comprises photodetectors placed in a ring configuration that detect gamma photons gen ...

Fluorescence lifetime imaging microscopy (FLIM) is an imaging modality often used to monitor biochemical properties of a cell or a tissue. In addition to conventional fluorescence microscopy features, such as selective labeling and non-invasiveness, FLIM e ...

Imaging methods to capture the beating and developing heart inside embryonic animal models such as the zebrafish are a key component for the study of fundamental biological processes such as cardiac birth defects or tissue regeneration. However, live heart ...

In this thesis, we study the 3 challenges described above. First, we study different reconstruction techniques and assess the fidelity of each reconstruction results by means of structured illumination and phase conjugation. By reconstructing the 3D refrac ...

Tim-domain near-infrared optical tomography (TD NIROT) techniques based on diffuse light were gaining performance over the last years. They are capable of imaging tissue at several centimeters depth and reveal clinically relevant information, such as tissu ...

Spectral imaging is a fundamental diagnostic technique with widespread application. Conventional spectral imaging approaches have intrinsic limitations on spatial and spectral resolutions due to the physical components they rely on. To overcome these physi ...

Time-domain optical coherence tomography with digital holographic microscopy

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Inverse Problems With Multiple Plane Waves: The Angular Simplification

Correlative light and electron microscopy for human brain and other biological models

An approach to characterize mechanisms of action of anti-amyloidogenic compounds in vitro and in situ

Live-cell imaging powered by computation

Subwavelength imaging using a solid-immersion diffractive optical processor

Subsurface fluorescence time-of-flight imaging using a large-format single-photon avalanche diode sensor for tumor depth assessment

Prognostic impact of deep learning-based quantification in clinical stage 0-I lung adenocarcinoma

Chemical Probe for Imaging of Polo-like Kinase 4 and Centrioles

Biophysical applications of correlative scanning probe and super-resolution microscopy

Practical computational imaging by use of spatiotemporal light modulation: from simulations to applications in biological microscopy

Towards quantum 3D imaging devices

Discomfort glare from daylight: Influence of transmitted color and the eye's macular pigment

Correlating cryo-super resolution radial fluctuations and dual-axis cryo-scanning transmission electron tomography to bridge the light-electron resolution gap

Intraocular Lens Refractive Index and Its Impact on External Surface Reflections

Integrated electronics for time-of-flight positron emission tomography photodetectors

Large-Format Time-Gated SPAD Cameras for Real-Time Phasor-Based FLIM

Computational methods for live heart imaging with speed-constrained microscopes

Advanced Techniques in Optical Diffraction Tomography

2.5 Hz sample rate time-domain near-infrared optical tomography based on SPAD-camera image tissue hemodynamics

High-Resolution Multi-Spectral Imaging With Diffractive Lenses and Learned Reconstruction