Publications associées à Dissecting the DNA binding principles of transcription factor monomers and heterodimers

Publications associées (30)

Thermally Assisted Microfluidics to Produce Chemically Equivalent Microgels with Tunable Network Morphologies

Although micron-sized microgels have become important building blocks in regenerative materials, offering decisive interactions with living matter, their chemical composition mostly significantly varies when their network morphology is tuned. Since cell be ...

Wiley-VCH Verlag GmbH2024

Tailoring the Hybridization Density of DNA Biosensors through Tunable Surface Functionalization

Pauline Skigin, Sandrine Gerber, Mounir Driss Mensi, Perrine Agnes Edith Robin, Lucas Mayoraz

DNA biosensors are promising candidates for the development of point-of-care diagnosis methods. They can be inserted in microfluidic platforms, are often non-expensive, and can be produced for a variety of targeted analytes. However, their development face ...

Wiley-VHCA AG2023

Quantification of successful encapsulation into microfluidic compartments

Christoph Merten

The invention provides a method for quantifying the number of target entities that were successfully encapsulated into a microfluidic compartment such as a microfluidic droplet. The invention is predicated upon a continuous quantifiable detectable signal c ...

2022

Towards scalable and deterministic single cell gene expression profiling

Johannes Julius Bues

Characterizing tissue composition at the cellular level is essential for understanding the human body in health and disease, and has been one of the frontiers in biology for centuries. Recently developed single-cell RNA-sequencing (scRNA-seq) techniques no ...

EPFL2021

Microfluidic culture platforms for C. elegans bacterial interaction and digestion studies at single-organism resolution

Vittorio Viri

Increasing interest in understanding fundamental biological processes, among which aging and nutrition, has led to the study of simple and powerful model organisms, as the round-worm Caenorhabditis elegans (C. elegans). The nematode, selected by Sydney Bre ...

EPFL2021

Inkjet Printing of Complex Soft Machines with Densely Integrated Electrostatic Actuators

Herbert Shea, Samuel Rosset, Samuel Schlatter, Giulio Grasso

A multimaterial inkjet printing method for integrated soft multifunctional machines is reported, combining dense arrays of electrostatic actuators, multilayer electrical routing, and complex networks of microfluidic channels in one printing process. Most a ...

2020

An integrated nanoplasmonic biosensor for monitoring cytokine secretion from single cells

Hatice Altug, George Coukos, Filiz Yesilköy, Alexander Belushkin, Xiaokang Li, Maria Soler Aznar

An innovative nanoplasmonic biosensor was presented for real-time label-free monitoring of cytokine secretion at single-cell resolution. This biosensor is integrated with a uniquely designed microfluidic device that provides a microchamber for cell incubat ...

IEEE2019

Cellular advective-diffusion drives the emergence of bacterial surface colonization patterns and heterogeneity

Alexandre Louis André Persat, Tamara Rossy

Microorganisms navigate and divide on surfaces to form multicellular structures called biofilms, the most widespread survival strategy found in the bacterial world. One common assumption is that cellular components guide the spatial architecture and arrang ...

Nature Research2019

Microfluidic device performing on flow study of serial cell–cell interactions of two cell populations

Clarisse Jeanne Antonia Benoit, Philippe Renaud, George Coukos, Alexandre Harari, Thamani Dahoun, Margaux Catherine Marie Duchamp

In this study we present a novel microfluidic hydrodynamic trapping device to probe the cell–cell interaction between all cell samples of two distinct populations. We have exploited an hydrodynamic trapping method using microfluidics to immobilize a batch ...

2019

Lab-on-a-chip technologies for manipulation and imaging of C. elegans worms and embryos

Li Dong

The nematode Caenorhabditis elegans is an attractive model organism, owing notably to its short life cycle, genetic tractability, and optical transparency facilitating microscopic observation. This thesis deals with the realization of technological tools f ...

EPFL2018

Memristive Biosensors Integration With Microfluidic Platform

Giovanni De Micheli, Sandro Carrara, Ioulia Tzouvadaki, Francesca Puppo

The integration of nanoscale sensors with microfluidic platforms is a powerful tool for the design of robust biosensing devices that present high reliability and the advantage of a quick data acquisition. In addition, microfluidic based lab-on-a-chip senso ...

Ieee-Inst Electrical Electronics Engineers Inc2016

System for optical detection and imaging of sub-diffraction-limited nano-objects

Martinus Adela Maria Gijs, Matteo Cornaglia, Hui Yang

Nano-objects (8) with feature size smaller than the classical Abbe's diffraction limit can be optically detected via a microlens (4) array configured in a miniaturized microfluidic device. A hydrophilic microwell (3) array in a hydrophobic layer (2) is fir ...

2016

Metal-Coated SU-8 Structures for High-Density 3-D Microelectrode Arrays

Carlotta Guiducci, Samuel Kilchenmann, Pietro Maoddi, Enrica Rollo

Electric fields can be effectively used to sense, manipulate, and move particles in lab-on-a-chip devices. Nevertheless, the throughput of such devices is a critical issue, which can be effectively improved by increasing the height of the microchannels. Fo ...

Institute of Electrical and Electronics Engineers2016

Continuous quantification of HER2 expression by microfluidic precision immunofluorescence estimates HER2 gene amplification in breast cancer

Martinus Adela Maria Gijs, Ata Tuna Ciftlik, Diego Gabriel Dupouy

Chromogenic immunohistochemistry (IHC) is omnipresent in cancer diagnosis, but has also been criticized for its technical limit in quantifying the level of protein expression on tissue sections, thus potentially masking clinically relevant data. Shifting f ...

Nature Research2016

A Simple and Reliable PDMS and SU-8 Irreversible Bonding Method and Its Application on a Microfluidic-MEA Device for Neuroscience Research

Philippe Renaud, Arnaud Bertsch, Patrick Fraering, Marc Olivier Heuschkel, Sébastien Mosser, Yufei Ren

Polydimethylsiloxane (PDMS) and SU-8 are currently two very commonly used polymeric materials in the microfluidics field for biological applications. However; there is a pressing need to find a simple, reliable, irreversible bonding method between these tw ...

MDPI2015

Quantitative single-cell analysis of S. cerevisiae using a microfluidic live-cell imaging platform

Johannes Becker

Genome-wide manipulations and measurements have made huge progress over the last decades. In Saccharomyces cerevisiae, a well-studied eukaryotic model organism, homologous recombination allows for systematic deletion or alteration of a majority of its gene ...

EPFL2015

Conformal Coating of Islets of Langerhans: a Microfluidic Approach for Transplantation Without Immunosuppression

Jeffrey Alan Hubbell, Alice Anna Tomei

Mary Ann Liebert, Inc2014

Scintillation detectors based on silicon microfluidic channels

Philippe Renaud, Alessandro Mapelli, Pietro Maoddi

Microfluidic channels obtained by SU-8 photolithography and filled with liquid scintillators were recently demonstrated to be an interesting technology for the implementation of novel particle detectors. The main advantages of this approach are the intrins ...

Institute of Physics2014

On-Chip Fabrication of Paclitaxel-Loaded Chitosan Nanoparticles for Cancer Therapeutics

Philippe Renaud, Arnaud Bertsch, Mohammad Mahdi Hasani-Sadrabadi, Jules John Vandersarl

The use of solvent-free microfluidics to fine-tune the physical and chemical properties of chitosan nanoparticles for drug delivery is demonstrated. Nanoparticle self-assembly is driven by pH changes in a water environment, which increases biocompatibility ...

Wiley-Blackwell2014

Delayed bactericidal response of Mycobacterium tuberculosis to bedaquiline involves remodelling of bacterial metabolism

John McKinney, Neeraj Dhar, Muhittin Emre Özdemir

Bedaquiline (BDQ), an ATP synthase inhibitor, is the first drug to be approved for treatment of multidrug-resistant tuberculosis in decades. Though BDQ has shown excellent efficacy in clinical trials, its early bactericidal activity during the first week o ...

Nature Publishing Group2014