Publications associées à Stiffness tomography exploration of living and fixed macrophages

Publications associées (36)

Morphodynamics of human early brain organoid development

Brain organoids enable the mechanistic study of human brain development and provide opportunities to explore self-organization in unconstrained developmental systems1, 2–3. Here we establish long-term, live light-sheet microscopy on unguided brain organoid ...

Springer Science and Business Media LLC2025

Investigating the effects of plasma-activated water on E. coli with the novel application of single-cell impedance flow cytometry

Ivo Furno, Fabio Avino, Rita Agus, Brayden Graham Myers

Single-cell impedance flow cytometry (IFC) is a rapid, label-free diagnostic capable of resolving the dielectric properties of individual cells. Here, single-cell IFC is employed to investigate the effects of plasma-activated water (PAW) on E. coli. This d ...

Springer Science and Business Media LLC2025

Advancements in Nanomechanical Characterization and Biomolecular Imaging with Atomic force Microscopy

Veronika Cencen

Atomic force microscopy (AFM) is a widely used imaging tool for obtaining a variety of information for a range of samples. Although it was initially intended to serve as a method of observing very flat solid surfaces, its use expanded into several other fi ...

EPFL2024

Computational drug development for membrane protein targets

Pierre Vogel, Henning Paul-Julius Stahlberg, Shuguang Yuan, Dongchun Ni, Babatunde Edukpe Ekundayo

The application of computational biology in drug development for membrane protein targets has experienced a boost from recent developments in deep learning-driven structure prediction, increased speed and resolution of structure elucidation, machine learni ...

2024

From Surfactants to 3D Printing: How Ion-Chelator Pair Interactions Affect the Mechanical Properties of Bioinspired Materials

Gaia De Angelis

The remarkable adaptability observed in marine ecosystems has often inspired researchers when developing new soft materials. The research undertaken in this thesis explores how ion chelator pair interactions influence the mechanical properties of bioinspir ...

EPFL2024

Coherently amplified ultrafast imaging using a free-electron interferometer

Fabrizio Carbone

Accessing the low-energy non-equilibrium dynamics of materials and their polaritons with simultaneous high spatial and temporal resolution has been a bold frontier of electron microscopy in recent years. One of the main challenges lies in the ability to re ...

Nature Publishing Group2024

Structure of a membrane-bound menaquinol:organohalide oxidoreductase

Christof Holliger, Henning Paul-Julius Stahlberg, Julien Maillard, Dongchun Ni, Lorenzo Cimmino, Babatunde Edukpe Ekundayo

Organohalide-respiring bacteria are key organisms for the bioremediation of soils and aquifers contaminated with halogenated organic compounds. The major players in this process are respiratory reductive dehalogenases, corrinoid enzymes that use organohali ...

Nature Research2023

Micro- or nanostructured optical element

Niels Quack, Dorian Giraud Herle

A micro- or nanostructured optical element (1) is proposed comprising: a membrane (3) with an array of holes (5), the membrane (3) comprising a membrane light wave facing surface; an array of pillars (7) sized and shaped such that a respective pillar (7) i ...

2023

Large Libraries of Structurally Diverse Macrocycles Suitable for Membrane Permeation

Christian Heinis, Alessandro Angelini, Alexander Lund Nielsen, Mischa Schüttel, Manuel Leonardo Merz, Xinjian Ji, Anne Sofie Luise Zarda, Ganesh Kumar Mothukuri, Zsolt Bognár, Edward Jeffrey Will

Macrocycles offer an attractive format for drug development due to their good binding properties and potential to cross cell membranes. To efficiently identify macrocyclic ligands for new targets, methods for the synthesis and screening of large combinator ...

Wiley-VCH Verlag GmbH2024

Homogenization theory captures macroscopic flow discontinuities across Janus membranes

Kevin Wittkowski, François Gallaire, Pier Giuseppe Ledda, Giuseppe Antonio Zampogna

Janus membranes, thin permeable structures with chemical and geometrical asymmetric properties, show great potential in industrial separation processes. Yet the link between the micro- and macro-scale behaviours of these membranes needs to be established r ...

Cambridge University Press2023

Rescue of secretion of rare-disease-associated misfolded mutant glycoproteins in UGGT1 knock-out mammalian cells

Maurizio Molinari

Endoplasmic reticulum (ER) retention of misfolded glycoproteins is mediated by the ER-localized eukaryotic glycoprotein secretion checkpoint, UDP-glucose glycoprotein glucosyl-transferase (UGGT). The enzyme recognizes a misfolded glycoprotein and flags it ...

Wiley2024

Membrane electrode assembly simulation of anion exchange membrane water electrolysis

Zoé Mury, Jan Van Herle, Suhas Nuggehalli Sampathkumar, Khaled Lawand

Anion exchange membrane water electrolysis (AEMWE) offers a green hydrogen production method that eliminates the need for platinum group metals (PGM) as electrocatalysts. This study employs a COMSOL (R) 6.0 model to simulate a 1x1 cm(2) Ni fibre - Raney (R ...

Elsevier2024

Peptide-based virucidal drugs

Francesca Olgiati

Before the COVID-19 pandemic, viruses were not a major priority for the scientific community. Today, many events have changed the world and the importance of studying viruses, vaccines, and antiviral drugs is fully appreciated. The development of effective ...

EPFL2024

PROBING INTRACELLULAR ELASTICITY WITH MINIMAL-HESSIAN REGISTRATION

Michaël Unser

We propose an image-based elastography method to measure the heterogeneous stiffness inside a cell and its nucleus. It uses a widely accessible setup consisting of plate compression imaged with fluorescence microscopy. Our framework recovers a spatial map ...

IEEE2023

S-acylation and lipid exchange at the ER-Golgi membrane contact sites regulate pathogen entry in human cells

Muhammad Umair Anwar

Cells have evolved endocytic pathways to internalize different molecules, to regulate intra-cellular communication and their interaction with external environment. Pathogens have co-evolved with cells to exploit these processes for infections. In particula ...

EPFL2024

Benzene with Alkyl Chains Is a Universal Scaffold for Multivalent Virucidal Antivirals

Jay-Ar Bautista Dela Cruz, Francesco Stellacci, Paulo Henrique Jacob Silva, Matteo Gasbarri, Lixia Wei, Yong Zhu, Heyun Wang, Sujeet Pawar

Most viruses start their invasion by binding to glycoproteins' moieties on the cell surface (heparan sulfate proteoglycans [HSPG] or sialic acid [SA]). Antivirals mimicking these moieties multivalently are known as broad-spectrum multivalent entry inhibito ...

Amer Chemical Soc2024

Engineered substrates incapable of induction of chondrogenic differentiation compared to the chondrocyte imprinted substrates

Philippe Renaud, Leila Montazeri

It is well established that surface topography can affect cell functions. However, finding a reproducible and reliable method for regulating stem cell behavior is still under investigation. It has been shown that cell imprinted substrates contain micro- an ...

IOP Publishing Ltd2023

Live-cell imaging powered by computation

Suliana Manley

The proliferation of microscopy methods for live-cell imaging offers many new possibilities for users but can also be challenging to navigate. The prevailing challenge in live-cell fluorescence microscopy is capturing intra-cellular dynamics while preservi ...

Nature Portfolio2024

Structural diversity of photoswitchable sphingolipids for optodynamic control of lipid microdomains

Samuel Mendes Leitão

Sphingolipids are a structurally diverse class of lipids predominantly found in the plasma membrane of eukaryotic cells. These lipids can laterally segregate with other rigid lipids and cholesterol into liquid-ordered domains that act as organizing centers ...

2023

Multiplicity-free Representations of Algebraic Groups

Donna Testerman

Let K be an algebraically closed field of characteristic zero, and let G be a connected reductive algebraic group over K. We address the problem of classifying triples (G, H, V ), where H is a proper connected subgroup of G, and V is a finitedimensional ir ...

Amer Mathematical Soc2024