Publications associées à Conformal Coating of Islets of Langerhans: a Microfluidic Approach for Transplantation Without Immunosuppression

Publications associées (28)

Harnessing deep learning to detect bronchiolitis obliterans syndrome from chest CT

Pascal Fua, Michele De Palma, Nahal Mansouri, Jakub Jan Gwizdala

BackgroundBronchiolitis Obliterans Syndrome (BOS), a fibrotic airway disease that may develop after lung transplantation, conventionally relies on pulmonary function tests (PFTs) for diagnosis due to limitations of CT imaging. Deep neural networks (DNNs) h ...

SPRINGERNATURE2025

Bioengineered Tubular Biliary Organoids

Matthias Lütolf, Saba Rezakhani, Mikhail Nikolaev, Bilge Sen Elçi

Liver organoids have emerged as promising in vitro models for toxicology, drug discovery, and disease modeling. However, conventional 3D epithelial organoid culture systems suffer from significant drawbacks, including limited culture duration, a nonphysiol ...

Wiley2024

Thermally Assisted Microfluidics to Produce Chemically Equivalent Microgels with Tunable Network Morphologies

Nikita Norkin

Although micron-sized microgels have become important building blocks in regenerative materials, offering decisive interactions with living matter, their chemical composition mostly significantly varies when their network morphology is tuned. Since cell be ...

Wiley-VCH Verlag GmbH2024

Tailoring the Hybridization Density of DNA Biosensors through Tunable Surface Functionalization

Pauline Skigin, Sandrine Gerber, Mounir Driss Mensi, Perrine Agnes Edith Robin, Lucas Mayoraz

DNA biosensors are promising candidates for the development of point-of-care diagnosis methods. They can be inserted in microfluidic platforms, are often non-expensive, and can be produced for a variety of targeted analytes. However, their development face ...

Wiley-VHCA AG2023

Quantification of successful encapsulation into microfluidic compartments

Christoph Merten

The invention provides a method for quantifying the number of target entities that were successfully encapsulated into a microfluidic compartment such as a microfluidic droplet. The invention is predicated upon a continuous quantifiable detectable signal c ...

2022

Microfluidic culture platforms for C. elegans bacterial interaction and digestion studies at single-organism resolution

Vittorio Viri

Increasing interest in understanding fundamental biological processes, among which aging and nutrition, has led to the study of simple and powerful model organisms, as the round-worm Caenorhabditis elegans (C. elegans). The nematode, selected by Sydney Bre ...

EPFL2021

Towards scalable and deterministic single cell gene expression profiling

Johannes Julius Bues

Characterizing tissue composition at the cellular level is essential for understanding the human body in health and disease, and has been one of the frontiers in biology for centuries. Recently developed single-cell RNA-sequencing (scRNA-seq) techniques no ...

EPFL2021

Inkjet Printing of Complex Soft Machines with Densely Integrated Electrostatic Actuators

Herbert Shea, Samuel Rosset, Samuel Schlatter, Giulio Grasso

A multimaterial inkjet printing method for integrated soft multifunctional machines is reported, combining dense arrays of electrostatic actuators, multilayer electrical routing, and complex networks of microfluidic channels in one printing process. Most a ...

2020

Hydrogel-based milliwell arrays for standardized and scalable retinal organoid cultures

Matthias Lütolf, Nathalie Brandenberg

The development of improved methods to culture retinal organoids is relevant for the investigation of mechanisms of retinal development under pathophysiological conditions, for screening of neuroprotective compounds, and for providing a cellular source for ...

Nature Research2020

An integrated nanoplasmonic biosensor for monitoring cytokine secretion from single cells

Hatice Altug, George Coukos, Filiz Yesilköy, Alexander Belushkin, Xiaokang Li, Maria Soler Aznar

An innovative nanoplasmonic biosensor was presented for real-time label-free monitoring of cytokine secretion at single-cell resolution. This biosensor is integrated with a uniquely designed microfluidic device that provides a microchamber for cell incubat ...

IEEE2019

Cellular advective-diffusion drives the emergence of bacterial surface colonization patterns and heterogeneity

Alexandre Louis André Persat, Tamara Rossy

Microorganisms navigate and divide on surfaces to form multicellular structures called biofilms, the most widespread survival strategy found in the bacterial world. One common assumption is that cellular components guide the spatial architecture and arrang ...

Nature Research2019

Microfluidic device performing on flow study of serial cell–cell interactions of two cell populations

Clarisse Jeanne Antonia Benoit, Philippe Renaud, George Coukos, Alexandre Harari, Thamani Dahoun, Margaux Catherine Marie Duchamp

In this study we present a novel microfluidic hydrodynamic trapping device to probe the cell–cell interaction between all cell samples of two distinct populations. We have exploited an hydrodynamic trapping method using microfluidics to immobilize a batch ...

2019

The Detection of Early Epigenetic Inheritance of Mitochondrial Stress in C. Elegans with a Microfluidic Phenotyping Platform

Martinus Adela Maria Gijs, Johan Auwerx, Matteo Cornaglia, Hüseyin Baris Atakan, Kevin Sebastiaan Hof

Fluctuations and deterioration in environmental conditions potentially have a phenotypic impact that extends over generations. Transgenerational epigenetics is the defined term for such intergenerational transient inheritance without an alteration in the D ...

Nature Research2019

Lab-on-a-chip technologies for manipulation and imaging of C. elegans worms and embryos

Li Dong

The nematode Caenorhabditis elegans is an attractive model organism, owing notably to its short life cycle, genetic tractability, and optical transparency facilitating microscopic observation. This thesis deals with the realization of technological tools f ...

EPFL2018

Interface-fitted moving mesh method for axisymmetric two-phase flow in microchannels

John Richard Thome, Gustavo Rabello Dos Anjos, Erik Gros

A boundary-fitted moving mesh scheme is presented for the simulation of two-phase flow in two-dimensional and axisymmetric geometries. The incompressible Navier-Stokes equations are solved using the finite element method, and the mini element is used to sa ...

Wiley2018

Microfluidic platforms for high-throughput mammalian cell printing, transfection, and dosage-dependent studies

Kristina Pan Woodruff

With the advent of high-throughput and genome-wide screening initiatives, there is a need for improved methods for cell-based assays. Current approaches require expensive equipment, rely on large-scale culturing formats not suited for small or rare sample ...

EPFL2017

Memristive Biosensors Integration With Microfluidic Platform

Giovanni De Micheli, Sandro Carrara, Ioulia Tzouvadaki, Francesca Puppo

The integration of nanoscale sensors with microfluidic platforms is a powerful tool for the design of robust biosensing devices that present high reliability and the advantage of a quick data acquisition. In addition, microfluidic based lab-on-a-chip senso ...

Ieee-Inst Electrical Electronics Engineers Inc2016

Metal-Coated SU-8 Structures for High-Density 3-D Microelectrode Arrays

Carlotta Guiducci, Samuel Kilchenmann, Pietro Maoddi, Enrica Rollo

Electric fields can be effectively used to sense, manipulate, and move particles in lab-on-a-chip devices. Nevertheless, the throughput of such devices is a critical issue, which can be effectively improved by increasing the height of the microchannels. Fo ...

Institute of Electrical and Electronics Engineers2016

System for optical detection and imaging of sub-diffraction-limited nano-objects

Martinus Adela Maria Gijs, Matteo Cornaglia, Hui Yang

Nano-objects (8) with feature size smaller than the classical Abbe's diffraction limit can be optically detected via a microlens (4) array configured in a miniaturized microfluidic device. A hydrophilic microwell (3) array in a hydrophobic layer (2) is fir ...

2016

Continuous quantification of HER2 expression by microfluidic precision immunofluorescence estimates HER2 gene amplification in breast cancer

Martinus Adela Maria Gijs, Ata Tuna Ciftlik, Diego Gabriel Dupouy

Chromogenic immunohistochemistry (IHC) is omnipresent in cancer diagnosis, but has also been criticized for its technical limit in quantifying the level of protein expression on tissue sections, thus potentially masking clinically relevant data. Shifting f ...

Nature Research2016