Super-resolution fight club: assessment of 2D and 3D single-molecule localization microscopy software

Chattez avec Graph Search

Posez n’importe quelle question sur les cours, conférences, exercices, recherches, actualités, etc. de l’EPFL ou essayez les exemples de questions ci-dessous.

AVERTISSEMENT : Le chatbot Graph n'est pas programmé pour fournir des réponses explicites ou catégoriques à vos questions. Il transforme plutôt vos questions en demandes API qui sont distribuées aux différents services informatiques officiellement administrés par l'EPFL. Son but est uniquement de collecter et de recommander des références pertinentes à des contenus que vous pouvez explorer pour vous aider à répondre à vos questions.

In unfocused ultrasound imaging, a delay-and-sum algorithm is commonly used to reconstruct one image per emission. When multiple emissions are performed, individual images can be combined by coherent compounding to improve image quality. Alternative method ...

Fluorescence confocal laser-scanning microscopy (LSM) is one of the most popular tools for life science research. This popularity is expected to grow thanks to single-photon array detectors tailored for LSM. These detectors offer unique single-photon spati ...

Fundamental properties of light unavoidably impose features on images collected using fluorescence microscopes. Accounting for these features is often critical in quantitatively interpreting microscopy images, especially those gathering information at scal ...

Significance: Fluorescence guidance is used clinically by surgeons to visualize anatomical and/or physiological phenomena in the surgical field that are difficult or impossible to detect by the naked eye. Such phenomena include tissue perfusion or molecula ...

Interferometric scattering (iSCAT) microscopy enables the label-free observation of biomolecules. Consequently, single-particle imaging and tracking with the iSCAT-based method known as mass photometry (MP) is a growing area of study. However, establishing ...

Cell segmentation is a critical step for quantitative single-cell analysis in microscopy images. Existing cell segmentation methods are often tailored to specific modalities or require manual interventions to specify hyper-parameters in different experimen ...

The proliferation of microscopy methods for live-cell imaging offers many new possibilities for users but can also be challenging to navigate. The prevailing challenge in live-cell fluorescence microscopy is capturing intra-cellular dynamics while preservi ...

After decades of technological advancements, high-speed atomic force microscopy (HS-AFM) has emerged as a powerful technique for visualizing dynamic processes. At the nanoscale, the AFM provides valuable insights into the sample by sensing minute interacti ...

The present knowledge of plunging hyperpycnal river plumes is mainly based on two-dimensional (confined) laboratory experiments. Several hypotheses on three-dimensional (unconfined) flow processes have been made, but not tested in situ. In this field study ...

Atomic force microscopy (AFM) is a widely used imaging tool for obtaining a variety of information for a range of samples. Although it was initially intended to serve as a method of observing very flat solid surfaces, its use expanded into several other fi ...

Software is going through a trust crisis. Privileged code is no longer trusted and processes insufficiently protect user code from unverified libraries. While usually treated separately, confidential computing and program compartmentalization are both symp ...

Collaborative learning algorithms, such as distributed SGD (or D-SGD), are prone to faulty machines that may deviate from their prescribed algorithm because of software or hardware bugs, poisoned data or malicious behaviors. While many solutions have been ...

Optical microscopy is an essential tool for biologists, who are often faced with the need to overcome the spatial and temporal resolution limitations of their devices to capture finer details. As upgrading imaging hardware is expensive, computational metho ...

Nanometric scale size oscillations seem to be a fundamental feature of all living organisms on Earth. Their detection usually requires complex and very sensitive devices. However, some recent studies demonstrated that very simple optical microscopes and de ...

Polo-like kinase (Plk4) is a serine/threonine-proteinkinase thatis essential for biogenesis of the centriole organelle and is enrichedat centrioles. Herein, we introduce Cen-TCO, a chemical probe basedon the Plk4 inhibitor centrinone, to image Plk4 and cen ...

We propose an image-based elastography method to measure the heterogeneous stiffness inside a cell and its nucleus. It uses a widely accessible setup consisting of plate compression imaged with fluorescence microscopy. Our framework recovers a spatial map ...

We discuss the properties of signal strength and integrated intensity in two-photon excitation confocal microscopy and image scanning microscopy. The resolution, optical sectioning and background rejection are all improved over nonconfocal two-photon micro ...

Tip-enhanced optical effects have enabled a wide palette of nanoscale investigation techniques and recently they were successfully used to modify and manipulate matter with nanoscale spatial resolution. We contribute to this latter area by reporting a viab ...

Recent advances in volume electron microscopy (EM) have been driving our thorough understanding of the brain architecture. Volume EM becomes increasingly powerful when cells and their subcellular structures that are imaged in light microscopy are correlate ...

Entanglement and correlation are at the basis of quantummechanicsand have been used in optics to create a framework for "ghostimaging". We propose that a similar scheme can be used in anelectron microscope to exploit the correlation of electrons with theco ...

Super-resolution fight club: assessment of 2D and 3D single-molecule localization microscopy software

Chattez avec Graph Search

Inverse Problems With Multiple Plane Waves: The Angular Simplification

Compact and effective photon-resolved image scanning microscope

Fluorescence microscopy: A statistics-optics perspective

Subsurface fluorescence time-of-flight imaging using a large-format single-photon avalanche diode sensor for tumor depth assessment

Surface passivation and functionalisation for mass photometry

The multimodality cell segmentation challenge: toward universal solutions

Live-cell imaging powered by computation

Advances in High-Speed, Multiparametric Atomic Force Microscopy

Hydro-sedimentary processes of a plunging hyperpycnal river plume revealed by synchronized remote imagery and gridded current measurements

Advancements in Nanomechanical Characterization and Biomolecular Imaging with Atomic force Microscopy

Creating Trust by Abolishing Hierarchies

Robust Collaborative Learning with Linear Gradient Overhead

Practical computational imaging by use of spatiotemporal light modulation: from simulations to applications in biological microscopy

Mitochondrial nanomotion measured by optical microscopy

Chemical Probe for Imaging of Polo-like Kinase 4 and Centrioles

PROBING INTRACELLULAR ELASTICITY WITH MINIMAL-HESSIAN REGISTRATION

Background Rejection in Two-Photon Fluorescence Image Scanning Microscopy

Nanoscale local modification of PMMA refractive index by tip-enhanced femtosecond pulsed laser irradiation

Correlative light and volume electron microscopy to study brain development

One-Dimensional "Ghost Imaging" in Electron Microscopy of Inelastically Scattered Electrons