Milena Maria Schuhmacher

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Studying the role of molecularly distinct lipid species in cell signaling remains challenging due to a scarcity of methods for performing quantitative lipid biochemistry in living cells. We have recently used lipid uncaging to quantify lipid -pro-tein affi ...

Peroxisomes are eukaryotic organelles that are essential for multiple metabolic pathways, including fatty acid oxidation, degradation of amino acids, and biosynthesis of ether lipids. Consequently, peroxisome dysfunction leads to pediatric-onset neurodegen ...

Lipid nanoparticles (LNPs) are among the most promising drug delivery carriers in research and development, with one major clinical application being messenger RNA (mRNA) vaccine. Current LNP production methods have the limit of generating low polydispersi ...

To test the predominant role of the non-vesicular route for retrograde transport in U-2 OS cells, we inhibited vesicular trafficking using Brefeldin A, which blocks COPI coat formation at Golgi membranes and Wortmannin, which broadly affects endosome forma ...

Milena Maria Schuhmacher

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Quantifying single-cell diacylglycerol signaling kinetics after uncaging

Fluorescent fatty acid conjugates for live cell imaging of peroxisomes

Facile lipid nanoparticle size engineering approach via controllable fusion induced by depletion forces

Quantitative imaging of species-specific lipid transport in mammalian cells - endocytosis inhibitors

MinD-like ATPase FlhG effects location and number of bacterial flagella during C-ring assembly