Publications de Gioele La Manno

Publications associées (23)

A cell fitness selection model for neuronal survival during development

Developmental cell death plays an important role in the construction of functional neural circuits. In vertebrates, the canonical view proposes a selection of the surviving neurons through stochastic competition for target-derived neurotrophic signals, imp ...

Nature Research2019

Brain matters: unveiling the distinct contributions of region, age, and sex to glia diversity and CNS function

Gioele La Manno, Zahra Moslehi

The myelinated white matter tracts of the central nervous system (CNS) are essential for fast transmission of electrical impulses and are often differentially affected in human neurodegenerative diseases across CNS region, age and sex. We hypothesize that ...

BMC2023

Self-organizing in vitro mouse neural tube organoids mimic embryonic development

Matthias Lütolf, Gioele La Manno, Irina Khven, Jisoo Park, Hao-An Hsiung

The embryonic neural tube is the origin of the entire adult nervous system, and disturbances in its development cause life-threatening birth defects. However, the study of mammalian neural tube development is limited by the lack of physiologically realisti ...

COMPANY BIOLOGISTS LTD2022

Molecular Diversity of Midbrain Development in Mouse, Human, and Stem Cells

Gioele La Manno, Jesper Ryge

Understanding human embryonic ventral midbrain is of major interest for Parkinson's disease. However, the cell types, their gene expression dynamics, and their relationship to commonly used rodent models remain to be defined. We performed single-cell RNA s ...

2016

Molecular architecture of the developing mouse brain

Gioele La Manno, Irina Khven, Alex Russell Lederer

The mammalian brain develops through a complex interplay of spatial cues generated by diffusible morphogens, cell-cell interactions and intrinsic genetic programs that result in probably more than a thousand distinct cell types. A complete understanding of ...

NATURE PORTFOLIO2021

Molecular profiling of stem cell-derived retinal pigment epithelial cell differentiation established for clinical translation

Gioele La Manno, Irina Khven, Alex Russell Lederer

Human embryonic stem cell-derived retinal pigment epithelial cells (hESC-RPE) are a promising cell source to treat age-related macular degeneration (AMD). Despite several ongoing clinical studies, a detailed mapping of transient cellular states during in v ...

CELL PRESS2022

Single-cell transcriptional logic of cell-fate specification and axon guidance in early-born retinal neurons

Gioele La Manno, Marion Leleu

Retinal ganglion cells (RGCs), cone photoreceptors (cones), horizontal cells and amacrine cells are the first classes of neurons produced in the retina. However, an important question is how this diversity of cell states is transcriptionally produced. Here ...

2019

Clinically compliant cryopreservation of differentiated retinal pigment epithelial cells

Cheng Zhao, Gioele La Manno, Alex Russell Lederer

Background aims: Age-related macular degeneration (AMD) is the most common cause of blindness in elderly patients within developed countries, affecting more than 190 million worldwide. In AMD, the retinal pigment epithelial (RPE) cell layer progressively d ...

Elsevier Sci Ltd2024

Applying single-cell and single-nucleus genomics to studies of cellular heterogeneity and cell fate transitions in the nervous system

Gioele La Manno

Single-cell and single-nucleus genomic approaches can provide unbiased and multimodal insights. Here, we discuss what constitutes a molecular cell atlas and how to leverage single-cell omics data to generate hypotheses and gain insights into cell transitio ...

NATURE PORTFOLIO2024

Spatial tissue profiling by imaging-free molecular tomography

Christian Gabriel Schneider, Gioele La Manno, Fabrice David, Halima Hannah Emilie Schede

Several techniques are currently being developed for spatially resolved omics profiling, but each new method requires the setup of specific detection strategies or specialized instrumentation. Here we describe an imaging-free framework to localize high-thr ...

2021

Sphingolipids control dermal fibroblast heterogeneity

Gioele La Manno, Olivier Burri, Romain Guiet, Giovanni D'Angelo, Galina Glousker, Irina Khven, Sylvia Ho, Laura Capolupo, Alex Russell Lederer

Human cells produce thousands of lipids that change during cell differentiation and can vary across individual cells of the same type. However, we are only starting to characterize the function of these cell-to-cell differences in lipid composition. Here, ...

American Association for the Advancement of Science2022

Spotiflow: accurate and efficient spot detection for fluorescence microscopy with deep stereographic flow regression

Emine Berna Durmus, Suliana Manley, Joachim Lingner, Gioele La Manno, Martin Weigert, Irina Khven, Can Aztekin, Eftychia Kyriacou, Albert Dominguez Mantes, Anjalie Olivia Schläppi, Georgios Tsissios, Evangelia Skoufa

Identification of spot-like structures in large, noisy microscopy images is a crucial step for many life-science applications. Imaging-based spatial transcriptomics (iST), in particular, relies on the precise detection of millions of transcripts in low sig ...

Nature Publishing Group2025

Heterogeneity and developmental dynamics of mammalian neocortical progenitors

Leila Haj Abdullah Alieh, Gioele La Manno, Antonio Herrera Camacho

The central nervous system develops from a pool of neural progenitors which, depending on their location and time of division, generate cells committed to differentiate into specific kinds of neurons or glia. In the last decades, the developmental neurobio ...

Elsevier2023

Cell type prioritization in single-cell data

Grégoire Courtine, Gioele La Manno, Quentin Barraud, Jordan Squair, Claudia Kathe, Matthieu Pierre Gautier, Mark Andrew Anderson, Thomas Haynes Hutson, Michael Alexander Skinnider, Marco Milano

The cell types affected by biological perturbations in complex tissues are uncovered by single-cell analysis. ...

NATURE PUBLISHING GROUP2021

Clonal relations in the mouse brain revealed by single-cell and spatial transcriptomics

Gioele La Manno

Ratz et al. present an easy-to-use method to barcode progenitor cells, enabling profiling of cell phenotypes and clonal relations using single-cell and spatial transcriptomics, providing an integrated approach for understanding brain architecture. ...

NATURE PORTFOLIO2022

Confronting false discoveries in single-cell differential expression

Grégoire Courtine, Gioele La Manno, Quentin Barraud, Jordan Squair, Matthieu Pierre Gautier, Mark Andrew Anderson, Nicholas David James, Thomas Haynes Hutson, Claudia Kathe, Michael Alexander Skinnider, Rémi Bernard René Hudelle

Differential expression analysis of single-cell transcriptomics allows scientists to dissect cell-type-specific responses to biological perturbations. Here, the authors show that many commonly used methods are biased and can produce false discoveries. ...

Nature Portfolio2021

The lipotype hypothesis

Gioele La Manno, Giovanni D'Angelo

Giovanni D'Angelo and Gioele La Manno present the concept of lipotypes, and suggest that lipid composition is an important regulator of cell fate. ...

NATURE PORTFOLIO2023

Statistical inference with a manifold-constrained RNA velocity model uncovers cell cycle speed modulations

Maxine Leonardi, Alessandro Valente, Felix Naef, Gioele La Manno, Irina Khven, Alex Russell Lederer, Colas Noé Droin, Lorenzo Talamanca, Albert Dominguez Mantes, Antonio Herrera Camacho

Across biological systems, cells undergo coordinated changes in gene expression, resulting in transcriptome dynamics that unfold within a low-dimensional manifold. While low-dimensional dynamics can be extracted using RNA velocity, these algorithms can be ...

Nature Publishing Group2024

The emergence and promise of single-cell temporal-omics approaches

Gioele La Manno, Alex Russell Lederer

Single-cell transcriptomics enables the measurement of gene expression in complex biological systems at the resolution of individual cells. Multivariate analysis of single-cell data helps describe the variation in expression accompanying cellular processes ...

CURRENT BIOLOGY LTD2020

The Key Players of CNS function: Exploring the Effects of Region, Age, and Sex on Human Glia Diversity

Gioele La Manno, Zahra Moslehi

Wiley2023