Stefano Davide Vianello

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We present a low-cost, do-it-yourself system for complexmammalian cell culture under dynamically changing medium formulations by integrating conventional multi-well tissue culture plates with simple microfluidic control and system automation. We demonstrat ...

In humans, mice, and other mammals key internal organs such as the gut, the lungs, the pancreas, and the liver all derive from the same embryonic tissue: the endoderm. The development of all of these structures thus depends on a same set of early cells, an ...

Gastruloids are 3D structures generated from pluripotent stem cells recapitulating fundamental principles of embryonic pattern formation. Using single-cell genomic analysis, we provide a resource mapping cell states and types during gastruloid development ...

Research in developmental biology has been recently enriched by a multitude of in vitro models recapitulating key milestones of mammalian embryogenesis. These models obviate the challenge posed by the inaccessibility of implanted embryos, multiply experime ...

Stefano Davide Vianello

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An automated do-it-yourself system for dynamic stem cell and organoid culture in standard multi-well plates

In vitro endoderm emergence and self-organisation in the absence of extraembryonic tissues and embryonic architecture

Multimodal characterization of murine gastruloid development

Understanding the Mechanobiology of Early Mammalian Development through Bioengineered Models