Michelle Susanne Frei

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This presentation describes an experimental approach for the detection of cavitation in hydraulic machines by use of ultrasonic signal analysis. Instead of using the high frequency pulses (typically 1MHz) only for transit time measurement different other s ...

The specific and covalent labeling of the protein HaloTag with fluorescent probes in living cells makes it a powerful tool for bioimaging. However, the irreversible attachment of the probe to HaloTag precludes imaging applications that require transient bi ...

Super-resolution fluorescence microscopy is a powerful tool to visualize biomolecules and cellular structures at the nanometer scale. Employing these techniques in living cells has opened up the possibility to study dynamic processes with unprecedented spa ...

Fluorescence microscopy is the method of choice to monitor dynamic processes in living cells due to its non-invasive nature. A variety of different fluorophores and labeling systems are currently used to selectively visualise structures or biomolecules of ...

Photoactivatable fluorophores are important for single-particle tracking and super-resolution microscopy. Here we present a photoactivatable fluorophore that forms a bright silicon rhodamine derivative through a light-dependent protonation. In contrast to ...

HaloTag variants offer distinct brightness and fluorescence lifetimes compared with HaloTag7 when labeled with rhodamines. These variants were used for multiplexed imaging with a single fluorophore and to create lifetime-based cell cycle indicators. ...

Michelle Susanne Frei

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The detection of cavitation in hydraulic machines by use of ultrasonic signal analysis

Exchangeable HaloTag Ligands for Super-Resolution Fluorescence Microscopy

Small-Molecule Fluorescent Probes for Live-Cell Super-Resolution Microscopy

Photoactivatable Silicon Rhodamines for Super-Resolution Microscopy

Photoactivation of silicon rhodamines via a light-induced protonation

Engineered HaloTag variants for fluorescence lifetime multiplexing