Johannes Julius Bues

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Characterizing tissue composition at the cellular level is essential for understanding the human body in health and disease, and has been one of the frontiers in biology for centuries. Recently developed single-cell RNA-sequencing (scRNA-seq) techniques no ...

Single-cell RNA-sequencing (scRNA-seq) has revolutionized biomedical research by enabling the in-depth analysis of cell-to-cell heterogeneity of tissues with unprecedented resolution. One of the catalyzing technologies is single cell droplet microfluidics, ...

The present invention concerns a microfluidic device or chip including at least one inlet for introducing at least one object into the device; an oil inlet for introducing an oil that supports droplet formation into the device or a droplet forming substanc ...

The adoption of microfluidics was fundamental to the development of cost-effective, high-throughput DNA sequencing. As the field progresses towards multi-omics, we reflect on the key concepts underlying microfluidics and how resulting engineering advances ...

DisCo is a deterministic droplet microfluidics tool for single-cell analysis on low cell input samples, which is demonstrated to profile individual intestinal organoids and in vivo-derived small tissues. ...

Johannes Julius Bues

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Towards scalable and deterministic single cell gene expression profiling

Simplified Drop-seq workflow with minimized bead loss using a bead capture and processing microfluidic chip

Device for high throughput single-cell studies

The evolution of DNA sequencing with microfluidics

Deterministic scRNA-seq captures variation in intestinal crypt and organoid composition

Engineering next-generation microfluidic technologies for single-cell phenomics