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The core of this thesis is the application of different modalities of atomic force microscopy to study living systems. After a brief introduction, quantitative imaging state of the art is presented and the applications for mammalian, plant, bacterial, and ...

Objectives: The management of bloodstream infection, a life-threatening disease, largely relies on early detection of infecting microorganisms and accurate determination of their antibiotic susceptibility to reduce both mortality and morbidity. Recently we ...

Background and Objectives: Optimization of chemotherapy is crucial for cancer patients. Timely and costly efficient treatments are emerging due to the increasing incidence of cancer worldwide. Here, we present a methodology of nano-motion analysis that cou ...

The existence of life in extreme conditions, in particular in extraterrestrial environments, is certainly one of the most intriguing scientific questions of our time. In this report, we demonstrate the use of an innovative nanoscale motion sensor in life-s ...

Our team recently demonstrated that cantilever based devices can detect signature of life in a chemistry independent manner. In this technique, the organism of interest is attached onto a classical AFM cantilever. If alive, it induces nanometre scale oscil ...

Bacterial adhesion is the first and a significant step in establishing infection. This adhesion normally occurs in the presence of flow of fluids. Therefore, bacterial adhesins must be able to provide high strength interactions with their target surface in ...

The insurgence of newly arising, rapidly developing health threats, such as drug-resistant bacteria and cancers, is one of the most urgent public-health issues of modern times. This menace calls for the development of sensitive and reliable diagnostic tool ...

Scanning electrochemical microscopy (SECM) has been widely used for the electrochemical imaging of dynamic topographical and metabolic changes in alive adherent mammalian cells. However, extracting intracellular information by SECM is challenging, since it ...

Atomic force microscopy is nowadays a well-established technique that permits the investigation of numerous parameters of living matter. In particular, it allows the exploration of the mechanical properties of living organisms in almost physiological condi ...

Petar Stupar

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Atomic Force Microscopy of Biological Systems: Quantitative Imaging and Nanomotion Detection

Nanomechanical sensor applied to blood culture pellets: a fast approach to determine the antibiotic susceptibility against agents of bloodstream infections

Nano-Motion Analysis for Rapid and Label Free Assessing of Cancer Cell Sensitivity to Chemotherapeutics

Detecting nanoscale vibrations as signature of life

Mitochondrial activity detected by cantilever based sensor

Localization of adhesins on the surface of a pathogenic bacterial envelope through atomic force microscopy

A perspective view on the nanomotion detection of living organisms and its features

Fixation and Permeabilization Approaches for Scanning Electrochemical Microscopy of Living Cells

AFM contribution to unveil pro- and eukaryotic cell mechanical properties

Detect the life through atomic force microscopy