Publications de Thomas Pengo | EPFL Graph Search

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The HIV structural protein Gag assembles to form spherical particles of radius ∼70 nm. During the assembly process, the number of Gag proteins increases over several orders of magnitude from a few at nucleation to thousands at completion. The challenge in ...

Super-resolution localization microscopy methods such as PALM and STORM have been shown to provide imaging with resolutions up to a few tens of nanometers while using relatively simple setups. Biplane PALM has extended the PALM technique to three-dimension ...

We created a high-throughput modality of photoactivated localization microscopy (PALM) that enables automated 3D PALM imaging of hundreds of synchronized bacteria during all stages of the cell cycle. We used high-throughput PALM to investigate the nanoscal ...

With the widespread uptake of two-dimensional (2D) and three-dimensional (3D) single-molecule localization microscopy (SMLM), a large set of different data analysis packages have been developed to generate super-resolution images. In a large community effo ...

During the past decade, localization microscopy (LM) has transformed into an accessible, commercially available technique for life sciences. However, data processing can be challenging to the non-specialist and care is still needed to produce meaningful re ...

Thomas Pengo

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Quantitative super-resolution imaging reveals protein stoichiometry and nanoscale morphology of assembling HIV-Gag virions

A Psf-Based Approach To Biplane Calibration In 3D Super-Resolution Microscopy

High throughput 3D super-resolution microscopy reveals Caulobacter crescentus in vivo Z-ring organization

Super-resolution fight club: assessment of 2D and 3D single-molecule localization microscopy software

PALMsiever: a tool to turn raw data into results for single-molecule localization microscopy

High throughput 3D Palm Imaging Elucidates Mechanisms of Bacterial Cell Division