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Melanie Blokesch

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Publications associées (61)

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Comparison of chitin‐induced natural transformation in pandemic Vibrio cholerae O1 El Tor strains

Melanie Blokesch, Sandrine Stutzmann

The human pathogen Vibrio cholerae serves as a model organism for many important processes ranging from pathogenesis to natural transformation, which has been extensively studied in this bacterium. Previous work has deciphered important regulatory circuits ...

2020

Sporadic type VI secretion in seventh pandemic Vibrio cholerae

Melanie Blokesch, Sandrine Stutzmann, Natalia Carolina Drebes Dorr, Nicolas René Chiaruttini, Loriane Bader, Sandrine Natacha Isaac, Lisa Christina Metzger, Alexis Joseph Proutière

Vibrio cholerae is a pathogen that causes disease in millions of people every year by colonizing the small intestine and then secreting the potent cholera toxin. How the pathogen overcomes the colonization barrier created by the host’s natural microbiota i ...

2023

TransFLP - A Method to Genetically Modify Vibrio cholerae Based on Natural Transformation and FLP-recombination

Melanie Blokesch

Several methods are available to manipulate bacterial chromosomes(1-3). Most of these protocols rely on the insertion of conditionally replicative plasmids (e.g. harboring pir-dependent or temperature-sensitive replicons(1,2)). These plasmids are integrate ...

2012

Neighbor predation linked to natural competence fosters the transfer of large genomic regions in Vibrio cholerae

Melanie Blokesch, Nicolas Jean Philippe Guex, Christian Iseli, Sandrine Stutzmann, Noémie Matthey, Candice Stoudmann

Natural competence for transformation is a primary mode of horizontal gene transfer. Competent bacteria are able to absorb free DNA from their surroundings and exchange this DNA against pieces of their own genome when sufficiently homologous. However, the ...

2019

Molecular insights into Vibrio cholerae’s intra-amoebal host-pathogen interactions

Audrey Sophie Vanhove, Graham Knott, Melanie Blokesch, Sandrine Stutzmann, Catherine Maclachlan, Natalia Carolina Drebes Dorr, Candice Stoudmann, Stéphanie Clerc, Charles Ulric V Van der Henst

Vibrio cholerae, which causes the diarrheal disease cholera, is a species of bacteria commonlyfound in aquatic habitats. Within such environments, the bacterium must defend itselfagainst predatory protozoan grazers. Amoebae are prominent grazers, withAcant ...

Nature Research2018

Natural competence for transformation

Melanie Blokesch

While most molecular biologists are familiar with the artificial transformation of bacteria in the context of laboratory cloning experiments, natural competence for transformation refers to a specific physiological state in which prokaryotes are able to ta ...

2016

West African–South American pandemic Vibrio cholerae encodes multiple distinct phage defence systems

Melanie Blokesch, Sandrine Stutzmann, David William Adams, Alexandre Lemopoulos, Milena Jadwiga Jaskolska, Loriane Bader, Laurie Righi

Our understanding of the factors underlying the evolutionary success of different lineages of pandemic Vibrio cholerae remains incomplete. The West African–South American (WASA) lineage of V. cholerae, responsible for the 1991–2001 Latin American cholera e ...

Springer Science and Business Media LLC2025

Two defence systems eliminate plasmids from seventh pandemic Vibrio cholerae

Melanie Blokesch, David William Adams, Milena Jadwiga Jaskolska

Horizontal gene transfer can trigger rapid shifts in bacterial evolution. Driven by a variety of mobile genetic elements—in particular bacteriophages and plasmids—the ability to share genes within and across species underpins the exceptional adaptability o ...

2022

Long-Read-Based Genome Sequences of Pandemic and Environmental Vibrio cholerae Strains

Melanie Blokesch, Natalia Carolina Drebes Dorr, Noémie Matthey

The bacterium Vibrio cholerae exhibits two distinct lifestyles, one as an aquatic bacterium and the other as the etiological agent of the pandemic human disease cholera. Here, we report closed genome sequences of two seventh pandemic V. cholerae O1 El Tor ...

2018

Quorum Sensing Contributes to Natural Transformation of Vibrio cholerae in a Species-Specific Manner

Melanie Blokesch, Patrick Martin Seitz

Although it is a human pathogen, Vibrio cholerae is a regular member of aquatic habitats, such as coastal regions and estuaries. Within these environments, V. cholerae often takes advantage of the abundance of zooplankton and their chitinous molts as a nut ...

American Society for Microbiology2011

Capsular polysaccharide restrains type VI secretion in Acinetobacter baumannii

Melanie Blokesch, Mary-Claude Croisier-Coeytaux, Nicolas Olmo Flaugnatti, Loriane Bader

The type VI secretion system (T6SS) is a sophisticated, contact-dependent nanomachine involved in interbacterial competition. To function effectively, the T6SS must penetrate the membranes of both attacker and target bacteria. Structures associated with th ...

2025

Bacterial type VI secretion system facilitates niche domination

Melanie Blokesch, Natalia Carolina Drebes Dorr

Over the last few decades humanity has experienced a series of tremendous technological advances, especially in the realm of basic science. A plethora of new approaches has allowed us to appreciate life in previously unimaginable detail, prompting the real ...

2018

Interbacterial predation as a strategy for DNA acquisition in naturally competent bacteria

Melanie Blokesch

Natural competence enables bacteria to take up exogenous DNA. The evolutionary function of natural competence remains controversial, as imported DNA can act as a source of substrates or can be integrated into the genome. Exogenous homologous DNA can also b ...

Nature Publishing Group2017

QstR-dependent regulation of natural competence and type VI secretion in Vibrio cholerae

Melanie Blokesch, Sandrine Stutzmann, Candice Stoudmann, Milena Jadwiga Jaskolska

During growth on chitinous surfaces in its natural aquatic environment Vibrio cholerae develops natural competence for transformation and kills neighboring non-immune bacteria using a type VI secretion system (T6SS). Activation of these two phenotypes requ ...

Oxford University Press2018

Composition of the DNA-uptake complex of Vibrio cholerae

Melanie Blokesch, Lisa Christina Metzger

Natural competence for transformation is a developmental program that allows certain bacteria to take up free extracellular DNA from the environment and integrate this DNA into their genome. Thereby, natural transformation acts as mode of horizontal gene t ...

2014

Growing away from monocultures – interdependent growth conditions for studying antibacterial and antiphage systems

Melanie Blokesch

2021

A Vibriophage Takes Antirepression to the Next Level

Melanie Blokesch, Leonardo Filipe Lemos Rocha

Since its isolation by Esther Lederberg, phage lambda and its repressor protein CI have contributed substantially to the advancement of molecular biology. In this issue of Cell Host & Microbe, Silpe et al. (2020) characterize the antirepressor Qtip of Vibr ...

Elsevier2020

Molecular mechanism of plasmid elimination by the DdmDE defense system

Melanie Blokesch, Sandrine Stutzmann, David William Adams, Laurie Righi

Seventh pandemic Vibrio cholerae strains contain two pathogenicity islands that encode the DNA defense modules DdmABC and DdmDE. Here we use cryogenic electron microscopy to reveal the mechanistic basis for plasmid defense by DdmDE. A structure of the DdmD ...

American Association for the Advancement of Science2024

A transmission model of the 2010 cholera epidemic in haiti

Andrea Rinaldo, Enrico Bertuzzo, Melanie Blokesch, Lorenzo Mari, Lorenzo Righetto

2011

Genome sequences of Vibrio cholerae strains isolated in the DRC between 2009 and 2012

Melanie Blokesch, Sandrine Stutzmann, Natalia Carolina Drebes Dorr, Alexandre Lemopoulos

Vibrio cholerae has caused seven cholera pandemics in the past two centuries. The seventh and ongoing pandemic has been particularly severe on the African continent. Here, we report long read-based genome sequences of six V. cholerae strains isolated in th ...

2024