Publications de Jean-Marc Busnel

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The present work reports on the quantification of total IgE in human serum using a microanalytical device whose fluidics is driven by gravity and capillary forces only. Thanks to the eight parallel microchannels in each microchip, calibration and sample an ...

In-source photocatalytic redox reactions based on a photosensitive target plate have been developed to realize peptide fragmentation during laser desorption ionization. Sample peptides and glucose are simply deposited on a spot of sintered TiO2 nanoparticl ...

Proteolysis with proteases preloaded within nanopores of porous material is a very fast process, where proteins can be digested in minutes compared to the conventional bulk enzyme reactions taking place over hours. To model this surprising phenomenon, a mo ...

A fountain pen probe has been developed to extend the scope of scanning electrochemical microscopy (SECM) experiments to dry surfaces. The fountain pen is fabricated by UV-photoablation of a polyethylene terephthalate (PET) film and consists on one side of ...

Finite element numerical simulations were carried out in 2D geometries to map the magnetic field and force distribution produced by rectangular permanent magnets as a function of their size and position with respect to a microchannel. A single magnet, two ...

An automated fraction collection interface has been developed for coupling capillary electrophoresis (CE) with matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). This fraction collection approach is based on electromigration and diff ...

Single-use aluminum foil-based laser desorption/ionization (LDI) target plates have been developed for mass spectrometry (MS) analysis and provide detection results comparable to those of commercial stainless steel plates while offering a convenient way to ...

Protein A-coated magnetic beads (0.3 μm) have been trapped in a small portion of a neutrally coated capillary (50 μm id). Anti-β-lactoglobulin (β-LG) antibodies have then been immobilized on the beads through strong affinity with protein A to subsequently ...

A soft stylus microelectrode probe has been developed to carry out scanning electrochemical microscopy (SECM) of rough, tilted, and large substrates in contact mode. It is fabricated by first ablating a microchannel in a polyethylene terephthalate thin fil ...

Carrier ampholyte-based capillary electrophoresis (CABCE) has recently been introduced as an alternative to CE (CZE) in the classical buffers. In this study, isoelectric BGEs were obtained by fractionation of Servalyt pH 4-9 carrier ampholytes to cuts of t ...

Capillary zone electrophoresis (CZE) and matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) are two techniques highly suitable for the separation and detection of intact proteins. Herein, based on the use of a recently introduced iont ...

A photosensitive plate based on sintered TiO2 nanoparticles has been developed to carry out in-source photo-induced reductions for cleavage of disulfide bridges using glucose as a hole scavenger during laser desorption ionization. ...

Capillary zone electrophoresis and carrier ampholytes based capillary electrophoresis have been used as a second separation step to Off-Gel isoelectric focusing for the analysis of complex peptide mixtures. A tryptic digest of four proteins (bovine serum a ...

Electrokinetic supercharging has been integrated in CZE for the development of a highly sensitive methodology for protein tryptic digest analysis. A careful choice of the experimental conditions led to sensitivity enhancement factors between 1000 and 10 00 ...

A specific multi-phosphopeptide enrichment method is presented to complement current phosphopeptide isolation strategies that normally bias mass spectrometry analysis towards the detection of mono-phosphopeptides. An adsorption model is developed that show ...

A magnetic beads based immunoaffinity capillary electrophoresis method for total Immunoglobulin E quantification in serum has been developed. The method combines speed, automation ability, and minimal sample consumption. Only 1 μL of serum is required whil ...

A compatible buffer system for the coupling of capillary electrophoresis (CE) with matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) was developed. The employed interface consists of a robot to drive a sliver-covered separation capil ...

A scanning electrochemical microscopy (SECM) protein detection methodology has been developed based on the tagging of free cysteines and other nucleophiles in proteins and peptides by benzoquinone. The tagged proteins are detected by the mediated reduction ...

Jean-Marc Busnel

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Total serum IgE quantification by microfluidic ELISA using magnetic beads

Photocatalytic Redox Reactions for In-Source Peptide Fragmentation

Kinetics of Proteolytic Reactions in Nanoporous Materials

Fountain pen for scanning electrochemical microscopy

Magnetic forces produced by rectangular permanent magnets in static microsystems

Iontophoretic Fraction Collection for Coupling Capillary Zone Electrophoresis with Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

TiO2 Printed Aluminum Foil: Single-Use Film for a Laser Desorption/Ionization Target Plate

Capillary electrophoresis immunoassay using magnetic beads

Soft Stylus Probes for Scanning Electrochemical Microscopy

Evaluation of carrier ampholyte-based capillary electrophoresis for separation of

High-sensitive protein analysis by FESI-CEMALDI-MS

In-source photocatalytic reduction of disulfide bonds during laser desorption ionization

Capillary Electrophoresis as a Second Dimension to Isoelectric Focusing for Peptide Separation

Electrokinetic supercharging for highly efficient peptide preconcentration in capillary zone electrophoresis

Controlling the specific enrichment of multi-phosphorylated peptides on oxide materials: aluminium foil as a target plate for laser desorption ionization mass spectrometry

Human fingerprint imaging by scanning electro-chemical microscopy

Magnetic Beads Based Immunoaffinity Capillary Electrophoresis of Total Serum IgE with Laser-Induced Fluorescence Detection

Compatible Buffer for Capillary Electrophoresis and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

Allergy Diagnostics Using Magnetic Beads in a GRAVI-Cell Microfluidic Device

Adsorbed protein detection by scanning electrochemical microscopy